Objective: To evaluate the effect of flotation therapy on the attention of undergraduates. Method: Subjects were 53 undergraduates (26 male, 27 female). They were randomly divided into 5 groups. Group 1,2,3 and group 4 were flotation groups and group 5 was control group. Each subject finished four therapies. Visual attention attribution and diversion (T5) and bilateral two- choice reaction time (T8) were used as the indexes. Result: As a whole, the T5 and T8 times of the subjects in the flotation groups were shortened in the course of the therapy. The promotion of attention in the male subjects was better than that in the female subjects. There was no significant difference between the changes of bilateral two-choice reaction time. The promotion in the flotation subjects was significantly better than that in the control subjects and it could not be explained totally by practice effect. Conclusion: The attention of the subjects can be promoted by flotation therapy. This study could not support the assumption that flotation therapy could promote the function of right brain as some prior studies declared.

Objective To retrospective analysis of the clinical effect and complications of the semi-laminectomy approach on the treatment of intra-spinal tumor.To summarize the endoscopic treatment experience of intra-spinal tumor.Methods To retrospective analysis of the clinical data of 24 cases with intra-spinal tumor in Shanxi Provincial People's Hospital from January 2008 to September 2015.The diagnosis of the intra-spinal tumor was confirmed by preoperative MRI examination.Intraoperative application of endoscopic surgery in the resection of tumor.Longterm followed up of patient was implemented and observe the efficacy and complications after surgery.Results Postoperative symptoms of all patients have different degrees of improvement,the degrees of sensory disorder was reduce,the limb muscle strength was increased and alleviate the damages of nerve electrophysiology.No serious complications were found after operation.Of the 24 patients,17 cases were total resection of the tumor and 7 cases were subtoal resection of the tumor.Tethered cord release was operated in patients with tethered spinal cord.Conclusions Endoscopic resection assisted semi-laminectomy approach of intra-spinal tumor has slight damage to the surround structures and the impact to the anatomy of the spine was small.Endoscopic assisted semi-laminectomy approach may have siginficant clinical effect on intra-spinal tumor,and have good security.

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, 20th Century ; History, 21st Century ; History, Ancient ; Humans ; Medicine in Literature

ADAM Proteins ; blood ; ADAMTS13 Protein ; Adult ; Case-Control Studies ; Female ; Fluorescence Resonance Energy Transfer ; Humans ; Male ; Middle Aged ; Purpura, Thrombotic Thrombocytopenic ; blood ; diagnosis ; Young Adult

Active constituents in Danshen and Compouud Danshen injections were determined by UV spectrometry and TLC scanning. Results showed that the total amount of catechol like constitucnts in injections obtaiued from thc market varies differeutly. It was proved that all the injectious containcd a large amount of tannius can lead to degeneration of red blood cells and agglutination of plasma protein and cause clinical toxic actions.

The change in pancreatic A- and B-cells in Chinese hamsters with spontaneous hereditary diabetes mellitus were investigated with specific immunohistochemical and image analyzing methods. A variety of pathological changes of the islets and change in number and distribution of the pancreatic A- and B-cells were found in diabetic Chinese hamsters. Microscopic examination revealed that the intensity of staining reaction of pancreatic B-cells varied greatly and the number of A-cells increased in this model. The results of image analysis showed that the range of the average optical density of B-cell staining was 54.4% wider than that in the control and the percentage of the glucagon positive immunoreactive area in the islet was significantly higher in diabetic Chinese hamsters(31.6?13.2%)than in the controls(21. 8?7.5%). These findings suggest that the islet cytology of this diabetic model changes significantly.

Objective To identify the immunogenicity and the potential of using Schistosoma japonicum mitochondria related protein(rSj338) as a candidate vaccine antigen for Schistosomiasis japonica . Methods The expressed recombinant fusion protein(rSj338/26GST) was purified and recognized by S.japonicum heavily infected rabbit sera and rSj338/26GST immunized rabbit sera by Western blotting. The inclusion bodies,the revivified protein and the purified protein from S 12 gel filtration were injected twice into rabbits to produce anti rSj338/26GST antibody which titer was then measured by dot ELISA. Balb/c mice were immunized with the inclusion bodies and the purified protein from S 12 gel filtration and challenged with S. japonicum cercariae to identify the protective immunity produced by rSj338/26GST. Results The inclusion bodies, the revivified protein and the purified protein from S 12 gel filtration could stimulate the rabbits to produce high level of anti rSj338/26GST antibodies and could be recognized by S. japonicum heavily infected rabbit sera and rSj338/26GST immunized rabbit sera. In Balb/c mice, the inclusion bodies could induce 27.8%( P

OBJECTIVE:To establish a HPLC method for determination of Vitamin B12 in compound vitamin capsules. METHODS:Compound vitamin capsules were determined by HPLC on a ODS column at room temperature.The mobile phase consisted of methyl alcohol and 0.05mol/L KH2PO4 buffer(22∶78,pH=6.4). The flow rate was 1.0ml/min,and the detection wave length was set at 360nm.The sample size was 20?l.RESULTS:The sample size of vitamin B12 presented a good linear relationship with peak area in the range of 10.20?g～204.00?g(r=0.9 999).The average recovery was 99.7%(RSD=1.18%).CONCLUSIONS:The present method is simple,convenient,and accurate for determination of vitamin B12 in compou_nd vitamin capsules,and it also has the advantage of preventing the interference from vitamin B6 and folic acid.

Nowadays partial splenic embolization (PSE) is an important therapeutic means to treat secondary hypersplenism caused by portal hypertension. By reducing the splenic blood flow and increasing hepatic blood supply, the peripheral blood picture and the liver function indexes as well as the portal hypertension can be effectively improved. This article aims mainly to make a comprehensive review on the therapeutic effect and mechanism of PSE, its clinical efficacy, as well as the factors affecting the clinical results, and to discuss the postoperative complications, the advantages and disadvantages of different embolic materials.

Objective To explore the effects of fibroblast transdifferentiation for myofibroblast (MFB) in the pathogenesis of systemic sclerosis (SSc) and to explore the antifibrotic mechanism of interferon γ (IFN-γ) in SSc.Methods The fibroblasts derived from the skin lesions of SSc patients and healthy adult controls were cultured in vitro and the MFB proportion in fibroblasts was examined by qualitative and quantitative α-smooth muscle actin (α-SMA) detection.By adding IFN-γ to the culture system with several doses,the influence on fibroblast proliferation and transdifferentiation for MFB in SSc was observed with MTT and enzyme linked immunosorbent assay (ELISA) respectively.Differences in the means of two independent samples were tested by Student' t-test.The means among multiple independent samples were com-pared by ANOVA.Results The means of positive α-SMA in SSc fibroblasts were higher than those in the controls (P＜0.01).With extended culture time,α-SMA levels of the two groups all increased gradually (P＜ 0.01 all),but there were higher α-SMA levels in SSc fibroblasts (24 h:130±19,48 h:183±21,72 h:249± 22) than those in controls (24 h:98±21,48 h:143±16,72 h:174±19) (P＜0.05 all).Although fibroblast proliferation and α-SMA levels were not influenced after adding of IFN-γ 10 U/ml (P＞0.05 all),but IFN-γ at concentration of 100 U/ml and 1000 U/ml could obviously repress fibroblast proliferation and α-SMA levels (P＜0.05 all),and 1000 U/ml had the strongest inhibiting effect at 24,48,72 h.Conclusion The fibroblasts in the skin of SSc patients have a strong potency to transdifferentiate to MFB.Early appropviate dose of IFN-γ could repress fibroblast proliferation and transdifferentiation in SSc.