ObjectiveTo evaluate the clinical efficiency of 0.1％ tacrolimus ointment combined with paroxetine in patients with neurodermatitis associated with insomnia.MethodsTotally,74 patients suffering from neurodermatitis and insomnia were consecutively enrolled in this study,and randomly assigned into the combination group and control group according to their number(odd or even).Both groups were given topical 0.1％ tacrolimus ointment twice a day,and the combination group was additionally given oral paroxetine 10 mg once a day,for 8 weeks.The outcome parameters included main symptoms of neurodermatitis and Pittsburgh sleep quality index(PSQI) score.ResultsNo significant difference was observed in the cure rate (54.3％(19/35) vs.53.1％ (17/32),P＞ 0.05) or response rate (82.9％ (29/35) vs.81.3％ (26/32),P＞ 0.05),between the combination group and control group.PSQI score was significantly lower in the combination group than in the control group after 8 weeks of treatment(P ＜ 0.05).ConclusionsThe 0.1％ tacrolimus ointment can be used to treat neurodermatitis safely.Antianxiety treatment can reduce the recurrence of neurodermatitis associated with insomnia,and improve the quality of sleep in patients.

Adult ; Female ; Humans ; Male ; Medical Staff, Hospital ; statistics & numerical data ; Middle Aged ; Nursing Staff, Hospital ; statistics & numerical data ; Sports ; statistics & numerical data

? AIM: To compare the effects on the retina inner segment and retinal pigment epithelium ( RPE ) of intravitreally injecting bevacizumab, ranibizumab and aflibercept into monkey eyes.? METHODS: Fourteen healthy cynomolgus monkeys (Macaca fascicularis, aged 3-8y,10 males,4 females) were raised at the Covance Laboratories under standard conditions. The 14 monkeys were grouped into 4 groups. Three of the groups with 4 monkeys each were injected intravitreally with one of the drugs, either bevacizumab, ranibizumab or aflibercept, while the 4th group with 2 monkeys served as a negative control. On 1d and 7d of injection, 2 monkeys from each drug treatment group were sacrificed under general anaesthesia and the 4 eyes were enucleated. All the enucleated eyes were fixed in formalin, embedded in paraffin wax, cut into 4. 0 μm sections and deparaffinized according to standard procedures. Image-Pro Plus was used for all the photos to measure the content of vascular endothelial growth factor ( VEGF) in the inner segment and RPE. The ANOVA test from JMP10. 0 statistical program was used to evaluate the results.?RESULTS:Retinal sections were checked for their anti-VEGF immune reactivity. The untreated control samples had the highest level of VEGF in the RPE and inner segment. All of these three drugs can reduce the level of VEGF in the RPE and inner segment, but Avastin seems to be more effective than Eylea in this regard. Lucentis treatment at 1d seems to be more effective than Eylea at VEGF 1d. But at 7d, both Lucentis and Eylea have the same effect on reducing VEGF expression level in the RPE and inner segment.?CONCLUSION: All of these three drugs can reduce the level of VEGF in the RPE and inner segment.

Objective To explore the effect of Linggui Zhugan decoction on the level of serum inflammatory factors , blood lipid and oxidation with coronary heart disease (CHD).Methods 123 cases of patients diagnosed as CHD and met criterions were collected from May 2013 to February 2015 in cardiovascular department of our hospital.They were divided into two groups according to different treatment modalities, observation group and control group.General information of the two groups were comparable, The patients of two groups reveived conventional treatments such as taking aspirin enteric-coated tablets, rosuvastatin, nitrate.The patients of observation group received Linggui Zhugan Decoction on the basis of conventional treatments.The serum levels of TC, TG, LDL-C, HDL-C, TNF-α, IL-6, IL-1β, SOD, MDA were recorded before and after treatment.Results The serum levels of TC, TG, LDL-C of observation groups were lower than those of control group after treatment (P<0.05).And the serum levels of HDL-C of observation groups were higher than those of control group after treatment (P<0.05).There was significant improvement in the serum levels of TNF-α, IL-6, IL-1β, MDA and SOD of two groups after treatment than those before treatment (P<0.05).The serum levels of TNF-α, IL-6, IL-1β, MDA of observation group were lower than those of control group after treatment ( P<0.05 ) , while the SOD serum levels of observation group were higher than those of control group after treatment (P<0.05).Conclusion Linggui Zhugan decoction could significantly improve the levels of blood lipids and decrease the levels of inflammation and oxidative stress in patients of CHD with significant effect.

Objective To depict the characteristics of the flow shape and distribution of superficial lymphadenophathy and to clarify the effiency of color Doppler flow imaging and power Doppler sonography in the differential diagnosis.Methods Prospectively,81 cases with 95 lesions were observed and clarified as four vascular patterns by sonography and were correlated with the histopathological or clinical diagnosis.The four vascular patterns were as follows: type Ⅰ shows central hilar vascularity with radial symmetric branches; type Ⅱ shows eccentric hilar vascularity with deformed radial branches; type Ⅲ shows subcapsular vessels(Ⅲa),mixed vessels(Ⅲb),or multifocal hilar vasculature(Ⅲc); type Ⅳ shows spotted or peripheral flow signals.Results The majority ( 85.0% ) of the benign reactive nodes revealed a vascular pattern of type Ⅰ,while a few ( 10.0% ) showed that of type Ⅱ.The most( 97.8% ) of lymphoma showed the patterns of type Ⅱ( 33.3% ) and type Ⅲ( 64.5% , and Ⅲa 28.9% ,Ⅲb 22.2% ,Ⅲb 13.4% ,respectively).The vascular patterns of metastatic lymphadenopathies were of type Ⅳ( 66.7% ) and type Ⅲb(26.7%).The lymphoma which revealed a vascular pattern of type Ⅱ were mostly of high grade differentiation in pathological results.The differrent vascular types of metastatic nodes has no correlation with its histologic source.Conclusions Color Doppler sonography is a valuable method for aid in the differential diagnosis of superficial lymphadenophathy by using findings of intranodal vascularity.

In order to arouse the working enthusiasm of the entire staff and give expression to the value of labor and skills, our hospital has implemented reform in the distribution system. Bonus given to clinical departments is linked both to the amount and quality of work and to economic profits; bonus to medico technical departments is given by means of deducting a percentage from the income and expenditure surplus in a way of progressive decrease; bonus given to functional departments is equivalent to 90% of the average bonus given to medical service departments; and bonus to logistical service departments is given, if possible, by the piece and mark. As a result of the reform, the distribution system has become more rational, the working enthusiasm and efficiency of the staff have been enhanced, operational income and income structure have improved, and social benefits bave been raised, with the satisfaction rate reaching over 90%. Of course, there still exist some difficulties in the reform.

Objective To investigate the association between microalbuminuria (MA) and high-sensitive C-reactive protein in essential hypertensive subjects. Methods Urinary albumin excretion rate (UAER)and the concentrations of high-sensitive C-reactive protein(hs-CRP) were measured. One hundred and eight patients with hypertension were divided into 2 groups: normoalbuminuria and microalbuminuria group. concentrations of hs-CRP was compared between the two groups , and the association between MA and hs-CRP was evaluated.Results Patients with microalbuminuria were significantly characterized by higher systolic blood pressure (SBP) ,pulse pressure(PP) ,circulating fibrinogen and hs-CRP. Evaluation of bivariate correlation indicated that UAER was positively correlated with SBP , PP , body mass index (BMI) ,Serum creatinine (SCr), fibrinogen and hs-CRP. Multivariate regression analysis demonstrated PP, SCr and hs-CRP were independent risks associated with UAER.Conclusions Microalbuminuria is a sensitive marker in essential hypertensive subjects with early renal injury .hs-CRP is an independent risk associated with UAER ,which suggests that inflammation correlate with early renal injury in essential hypertension.

Objective To study the effects of dexamethasone(Dex) on expression of aquaporin-4(AQP4) in rabbit non-pigmented ciliary epithelium(NPCE).Methods To construct rabbit corticosteroid-induced glaucoma model and then compare the differences of AQP4 expression in NPCE with normal controls by immunohistochemistry and immunofluorescence methods,as well as Western blot method.Results The immunohistochemistry results indicated that the AQP4 expression in NPCE of DEX group(OD=0.119?0.072) was suppressed(0.242?0.056)(P

Selenium, a metalloid mineral micronutrient,is an essential component for the adequate and healthy life of human, animals, and some other microorganisms.Selenium has antioxidative and oxidative effects on living organisms.Selenium deficiency has been associated with impaired function of the immune system.Selenium deficiency appears to enhance the virulence or progression of some viral infections.Selenium deficiency results in decreased activity of glutathione peroxidase, increasing oxidative damage and the likelihood of mutations in the viral genome.The safe range of selenium intake is relatively small and excess selenium intake can readily result in toxicity.This review summarizes the updates of benefitial and adverse effects of selenium on health.

Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity.Methods GM-CSF gene eukaryotic expressing plasmid was constructed by subclone and recombinant was transfected into FBL-3 cells by electroporation. After screening by G418 and cloning by limiting dilution,we obtained positive cell clones(FBL-3-GM-CSF). PCR and RT-PCR were used to identify the integration and stable expression of GM-SF gene in FBL-3-GM-CSF cells. The biological activity was confirmed by the hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. Results Mouse GM-CSF cDNA was amplified from the prokaryotic expressing plasmid PET-30a(+)-GM-CSF by PCR firstly and BamH Ⅰ and EcoRⅠrestriction sites were introduced. The inserted fragment was cut by BamH Ⅰ and EcoR Ⅰ digestion and ligated into pcDNA3 vector. The pcDNA3-GM-CSF eukaryotic expressing plasmid was constructed. The recombinant was cleared with appropriate endoneucleases and sequenced. The findings showed that the orientation of the insert was correct, while no rearrangement or mutation was found. PCR and RT-PCR assay showed that GM-CSF gene had integrated into FBL-3-GM-CSF cells and stably expressed. The hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay demonstrated that the cultured supernatant of FBL-3-GM-CSF cells of expressing GM-CSF should obviously stimulate proliferation of murine marrow mononuclear cells, and could stimulate hematopoietic progenitor cell colony formation. The number of colony formation was 54.67?4.83. The rate of colony formation was 0.547 %.Conclusions GM-CSF gene eukaryotic expressing plasmid is constructed successfully. A cell clone, which can express stably GM-CSF gene and possess biological activity,is obtained. Our studies have founded the base for the preparation of GM-CSF gene-modified vaccine of tumor cell and the study of feasibility of immune therapy of leukemia.