Evidence-based medicine,personalization,standardization are the three common sense of cancer care in the 21st century.The model of cancer treatment has changed from experience-guided treatment into gene guided personalized treatment.Recently,personalized treatment of cancer develops fast,particularly in molecular profile for prognosis,molecular marker for efficiency of target drug and so on.

?AlM: To discuss the artificial tears on the tear film of diabetic patients with dry eye preoperatively, and the influence on the tear film’s fuctional after phacoemulsification.
?METHODS: Fifty-four diabetic patients with dry eye (60 eyes) were followed up before phacoemulsification. Preoperatively, group A ( 30 eyes in 28 cases ) was treated with Hydroxyl lndican eye drops and group B (30 eyes in 26 cases) was not treated. Postoperatively, both group A and B were treated with Tobramycin Dexamethasone eye drops, Oprah Winfrey lbuprofen eye drops and Hydroxyl lndican eye drops. Dry eye symptoms, break up time (BUT), SchirmerItest (SIt), fluorescein stain test ( Fl ) were measured at 3d preoperatively, and 1, 7, 30, 90d postoperatively.
? RESULTS: At 3d preoperatively, there was no statistical differences between the two groups for dry eye symptoms, BUT, S I t, Fl ( P>0. 05 ). At 1 and 7d postoperatively, there were significant statistical differents between the two groups for dry eye symptoms (P<0. 05). And dry eye symptoms scores of group A were less than that of group B. At 1, 7, 30d postoperatively, BUT was longer, FL was less, Slt was increased in group A, with significant statistical differences (all P<0. 05). At 90d postoperatively, two groups of dry eye symptoms, BUT, S I t, Fl had no statistical significance (P>0. 05).
? CONCLUSlON: Using artificial tears before phacoemulsification can improve symptoms of the diabetic patients with dry eye and accelerate the recovery of the tear film.

Physical activity is limited in maintenance hemodialysis (MHD) patients, and higher level of physical activity may result in less risk of death, milder depression or ahypnosis, less fatigue and better quality of life. Physical activity may relate with many factors. MHD patients may be benefited from exercise to improve the physical activity, mainly with aerobic exercise and resistance training during hemodialysis. Nurses can do more in health education and direction of exercise for MHD patients.

Objective To investigate the nalaxone treating first acute right heart failure.Methods52 patients with FARHF were divided into the naloxone group(30cases)and control group(20cases)at random.Patients in control group were given general comprehensively treating.Patients in naloxone group were given naloxone as well as comprehensively treating,were given intravenously 0.8 mg of first dose,if it was necessary,repeated 0.8 mg of dose,and continuously dropped for 1.2 mg was added into liquid 500 mL.After treatment of 24 hours,were compared the changes of HR、SBP、SV and heart function.ResultsIn the naloxone group,the total effective rates were 84.4%,the mortality rates were 6.6%;in control group.The total effective rates were 60%,the mortality rates were 18.3%.The difference of the effective rate of the naloxone group be treatment was statistically significant(P

Objective:To explore the relationship between glycosylated hemoglobin (HbA1c)and carotid artery atheroscle-rosis in aged patients with normal glucose tolerance.Methods:A total of 100 aged patients with normal glucose tolerance were selected.Their bilateral carotid arteries were measured by color Doppler ultrasonography.They were grouped accord-ing to carotid intima-media thickness (C-IMT,normal,thicken and atherosclerosis)and plaque form (no plaque,hard plaque and soft plaque);blood pressure,blood lipids and HbA1c etc.were compared among groups.Results: (1)Along with CIMT rose,there was significant increase in HbA1c level [(4.98 ± 0.55)% vs.(5.51 ± 0.42)% vs.(5.92 ± 0.39)%],and there was significant difference between any two groups (P<0.01 all);(2)HbA1c level was (5.36±0.51)%,(5.89±0.44)% and (5.97±0.2)% in no plaque group,hard plaque group and soft plaque group respectively,and there was significant difference among hard plaque group,soft plaque group and no plaque group (P <0.01 all).Conclu-sion:Glycosylated hemoglobin may become an important index assessing carotid atherosclerosis in aged people.

Forty-eight patients with essential hypertension or coronary disease in cardiac function NYHA class I underwent a routine electrocardiography and a cotour Dopper echocardiography were studied,and by multiple stepwise regression analysis irwas found that significant partial correlations exist between PTFVl and left atrial dimensionas wee as E peak,Macruz index and A peak.Macruz index≥1.6 gave a sensitivity of 71%VS accuracy 64% for A peak increase(>70 cm/s),of 61% vs 58% for A/E>1 respectively,.These results suggest that electrocardiographlic indexes of abnormality of left atriun combined with Colour Doppler echocardiograpby may be more helpful to predict wkether the latent left ventricutar diastolic dysfunction or decrease of diastolic reserve was present or absent in patients with essential hypertension or coronary artery disease in normal clinical cardiac function.

Aberrant promoter methylation in special genes such as tumor suppressor genes is early eventin carcinogenesis of lung cancer. Aberrant methylation, resulting in corresponding mRNA silence or overexpression , closely relates to carcinogenesis, development, treatment response and prognosis of lung cancer and it may become a potential biomarker. Methylation of special genes may provide evidences for individual treatment of lung cancers.

Objective To observe the effects of puerarin on the expression of glycogen synthase kinase- 3 (GSK- 3) in the skeletal muscle of rats with insulin resistance induced by high fat and sucrose diets. Methods 30 Wistar rats were randomly divided into three groups: normal group, model group and puerarin group, 10 rats in each group. The model group and puerarin group were fed with the high fat and sucrose diets for 4 weeks. Then puerarin group was treated with puerarin by abdominal injection (100 mg/kg) for 6 weeks. At the end of the experiment, GSK- 3 content was detected by Western blot analysis. Body weight, serum triglyceride and cholesterol levels, fasting plasma glucose and serum insulin concentrations were measured regularly and insulin sensitivity index was also computed as well. Results The outcome of Western blot analysis showed that the expression of GSK- 3 in the skeletal muscle of the model group increased by 70.20 % (P

Objective To find the key proteins associated with metastasis of ovarian cancer, and find potential diagnostic markers and therapeutic targets of this malignancy. Methods A comparative proteomic strategy, in a combination of two-dimensional electrophoresis separation and mass spectrometry identification, was adopted to search for proteome alternations in an ovarian cancer mother cell line HO-8910 and its highly metastatic cell subline HO-8910PM. Results Twenty-one significantly different spots (two-fold increase or decrease) were detected between the two cell lines, of which 17 candidate proteins were successfully identified and characterized. Compared with those in HO-8910 mother cell line, 16 proteins were significantly up-regulated, while 5 proteins down-regulated in the highly metastatic cell subline HO-8910PM. The seventeen identified proteins could be functionally classified into 7 groups as following: zinc finger protein, calcium-binding protein, DNA repair and synthesis protein, cell regulatory protein, metabolism-related protein, cell surface antigen, cell signals and transducing protein. Conclusions The results suggest that an obviously differential proteomic expression exists between the human ovarian cancer mother cell line HO-8910 and highly metastatic cell subline HO-8910PM. It provides a clue for further identification of metastasis-related proteins, novel diagnostic markers as well as therapeutic targets of this malignancy.

A PCR assay used for detection and quantification of Neotyphodium lolii in tissues of the grass Lolium perenne L. was developed. Compared with the Microscopic analysis of stained tissues, this method was more accurate. For quantitative applications, real time PCR was used to quantify Neotyphodium lolii in Lolium perenne L.. The results showed that there were some variations between different individuals, but no variance existed between different tillers from the same plant. Thus it can be concluded that fungal content in the plant is not only associated with species/variety specific, but also with the genotype of the host plant as well.