Postpartum stress incidence rate is quite high (26% - 85%). Several studies reveal that more than 50% of women experience stress and depression after giving birth and almost 80% of new mothers experience terrible sadness feelings after giving birth, often called postpartum stress. This study aims to determine the effect of postpartum coping skill classes (PCSC) on stress level, cortisol level, maternal self-efficacy (MSE), and baby's growth and development. This study employed an experimental design method with a randomized study pre-test post-test control group design. The study was conducted on 18 postpartum women who were given PCSC treatment which was integrated into postpartum classes and 17 women were given postpartum classes (PC) only. The study was conducted in 4 weeks. The data were analyzed statistically using a t-test method. Ethical clearance for the study was obtained. The results disclose that PCSC significantly decrease the PSS score (-2.66 ± 4.74, p = 0,029). Similarly, the cortisol level drops significantly (-38.53 ± 74.60, p = 0.031). In other respect, the MSE score between groups have significant mean difference (p = 0.013), but the weight gain and development of the baby between the two groups have no significant mean difference (p >0.05). The provision of coping skill classes through maternal classes for postpartum women was effective in decreasing stress levels, cortisol levels, and increasing MSE scores although it did not affect the baby's growth and development.

Introduction: High fat diet (HFD) can cause lipid accumulation and contribute to various metabolic disorders. Single clove garlic oil (SCGO) has advantages over regular garlic due to its higher amounts of organosulfide compounds in particular. This study aimed to determine the ability of SCGO extract to ameliorate hepatic steatosis and improve oxidative status by modulating expression of tumour necrosis factor α and superoxide dismutase in mice fed a HFD. Methods: Twenty-four adult male Balb/C mice were divided into six groups: i) normal diet; ii) positive control diet; iii) negative control diet; and iv) HFD with SCGO at 12.5 mg/kg body weight (mg/kg BW); v) HFD with SCGO at 25 mg/kg BW, vi) HFD with SCGO at 50 mg/kg BW. Liver weight and morphology, spleen weight, serum levels of superoxide dismutase (SOD) and tumour necrosis factor α (TNF-α), TNF-α expression in the aorta and lipid profiles were assessed at the end of the experimental period. Results: SCGO treatment was associated with significant decreases in liver and spleen weight as well as amelioration of hepatic steatosis. SCGO treatment also decreased TNF-α levels and expression. Serum levels of SOD in the SCGO groups were significantly increased compared with the negative control group. Lipid profiles were improved in the SCGO treatment groups compared with the negative control group. Conclusion: SCGO as an herbal medicine could be an effective treatment for degenerative disorders caused by HFD.

Aims: Biofilm is an assemblage of microorganisms enclosed in a matrix of extracellular materials, such as, extracellular polysaccharide (EPS), and relates to bacterial virulence, pathogenesis, and environmental survival. Bacteria inside biofilm are more resistant to conventional antibiotics and the host immune system. Non-biocidal antibiofilm compounds have been developed to address this problem. Specifically, actinomycetes have known to produce many metabolite compounds that have useful application in medicine and biotechnology. The study aimed to characterize bioactive compounds from actinomycetes crude extract that have capability as a multispecies antibiofilm agent. Methodology and results: In this study, none of the isolates had shown any antimicrobial activity. Based on the antibiofilm assay, most of the isolates have the capability to inhibit and to destroy biofilm formation of pathogenic bacteria at a 5% and 10% dosage. The crude extracts showing the highest activity for antibiofilm inhibition were extracted from Streptomyces sp. The characterization of the bioactive compounds showed that different components of a particular isolate responsible for its antibiofilm activity against pathogenic bacteria. The SW19 isolate had a nucleic acid, KP12 isolate had a combination of the three component (polysaccharide, protein, and nucleic acid), and CW17 isolate had a combination of polysaccharide and nucleic acid as the active compound for antibiofilm activity. Conclusion, significance and impact study: Thus, the bioactive crude extracts from actinomycetes has high potential to be used in treating biofilm-related infection and further research is needed to purify the bioactive compound from the crude extract which has antibiofilm activity against Gram-positive and Gram-negative pathogens.
Biofilms

Objective: Exercise is a risk factor for infertility in women. However, research on the effects of different intensities of exercise on folliculogenesis has not yielded clear results. This study was conducted to analyze the effects of differences in the intensity of exercise on folliculogenesis in mice. Methods: Nineteen female BALB/c mice (age, 3–4 months; weight, 13–25 g) were randomly divided into four groups: control, mild exercise, moderate exercise, and high-intensity exercise. The mice in the exercise groups engaged in swimming, with additional loads of 3%, 6%, or 9% of body weight, respectively. There were five swimming sessions per week for 4 weeks, with a gradually increasing duration every week. At the end of the treatment, ovarian extraction was carried out and hematoxylin and eosin staining was performed to identify folliculogenesis. Results: There were significant differences in the number of total follicles between the control and moderate-exercise groups (p=0.036) and between the mild- and moderate-exercise groups (p=0.005). The mean number of primary follicles was higher in the moderate-exercise group than in the mild-exercise group (p=0.006). The mean number of secondary, tertiary, and Graafian follicles did not differ significantly among groups (p≥0.05). However, the number of total follicles and follicles in each phase tended to increase after exercise, especially moderate-intensity exercise. Conclusion Exercise of different intensities affected the total number of follicles and primary follicles. The number of follicles of each phase tended to increase after exercise. Moderate-intensity exercise had better effects than other intensities of exercise.

Objective: To monitor the physiological characteristics and genes expression of obesity rat model after rambutan peel extract (RPE) treatment. Methods: Twenty-four 12-week-old male rats were divided into 4 groups: normal, obesity, obesity treated with ellagic acid (O-EA) and obesity treated with RPE30 (O-RPE30). Physiological characteristics were monitored by measuring body weight, calorie intake, size of adipocyte and level of triglyceride. Peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding proteins α and fatty acid binding protein 4 (FABP4) expression were observed using immunohistochemistry, Western blotting and quantitative RT-PCR methods. Results: Body weight gain of O-EA and O-RPE30 rats were lower than obesity group and size of adipocyte cells were smaller than obesity group ( P < 0.05), but when we compared to normal group, those groups had higher body weight gain and larger adipocyte cells. The level of triglycerides, protein expression of PPARγ and mRNA level of FABP4 genes were significantly downregulated on O-EA and O-RPE30 compared to obesity group ( P < 0.05). Our results indicated that RPE had potential substance as inhibitor of body weight gain, declining of size of adipocyte, level of triglycerides, PPARγ expression and mRNA level of FABP4 gene on obesity rat model. Conclusions: RPE have anti-obesity activity by inhibiting body weight gain, declining size of adipocyte, decreasing triglyceride, PPARγ expression and mRNA level of FABP4 gene on obesity rat model.

Aims: To identify mold contaminant on salted fish, from two different market locations (Kenjeran market, Surabaya and Beringharjo market, Yogyakarta). Furthermore, levels of AFB1 (aflatoxin B1) in salted fish samples were assayed. Methodology and results: The samples were cultivated on DRBC (Dichloran Rose Bengal Chloramphenicol Agar) and DG-18 (Dichloran (18%) Glycerol Agar) medium for enumeration, then transferred on MEA (Malt Extract Agar) medium for isolation and identification, followed by ELISA test to measure the AFB1 level. Meanwhile aflatoxin biosynthesis correlated genes (i.e. aflR, nor-1 and omtB genes) were identified using Polymerase Chain Reaction (PCR) method. The results showed that Aspergillus tamarii and A. flavus being contaminant on salted fish along with A. sydowii, A. niger, A. versicolor, Penicillium citrinum, and P. chrysogenum. Rhizopus sp. contamination was also found. AFB 1 was positively detected in all of samples with the highest concentration measured was 75.81 μg/kg which belong to Lidah salted fish and the lowest concentration measured was 4.33 μg/kg which belong to Rese salted fish. The suspected A. flavus and A. tamarii isolated from salted fish was positively detected in the presence of aflR, nor-1 and omtB genes. Conclusion, significance and impact of study Mold contamination was detected in salted fish from two different markets and all of those samples were contaminated by AFB1. These can be important information related to food safety aspect for salted fish.

Aims: This study was conducted to observe the stability of Spondias pinnata leaf (SPL) extract antibacterial at different pH, salt concentration and temperature, to examine its antibacterial effectivity on minced fish, and to determine its fraction’s antibacterial mechanism. Methodology and results: The tested SPL ethanolic extract, n-hexane, chloroform, ethyl acetate and water fractions’ antibacterial activity against Bacillus cereus and Vibrio parahaemolitycus. Its stability against pH, salt, and thermal variation was studied, as well as the mechanism and application in fish. Ethyl acetate fraction and water fraction showed the highest activity against B. cereus (MIC 0.62 mg/mL). Protein profile analysis using gel electrophoresis showed that B. cereus cells exposed with SPL ethyl acetate fraction and water fraction showed thinner protein bands as compared to control. Severe damage of the cells treated with 3 MIC was also observed under SEM. Antibacterial activity of SPL ethanolic against Bacillus cereus and Vibrio parahaemolitycus were stable against heat treatment (80–121 °C for 15 min) and NaCl treatment (0–10% w/v), whereas the inhibition zone respectively at pH 4 (10.31±0.25 and 8.09±0.97 mm) was higher than pH 7 (8.45±0.52 and 6.66±1.84 mm). Application of SPL ethanolic extract in fish broth showed higher antibacterial activity than in fish flesh, which gave bactericidal effect at 3 MIC. Conclusion, significance and impact of study Ethanol extract can be developed as a natural preservative in fish

Objectives: This study was conducted to document the development and usability testing of SIKRIBO, a tuberculosis screening application. Methods: The SIKRIBO application was developed using design science research methodology, which has six steps: problem identification and motivation, definition of objectives for a solution, product design and development, demonstration, evaluation, and communication. A system usability scale (SUS) questionnaire was used to assess application usability. A total of 20 health cadres (trained community members) and health workers participated in the usability tests. Results: Two versions of the application were developed: Android-based for users and web-based for administrators. The Android-based version has four main menus: Find Tuberculosis, Tuberculosis Education, Latest Info, and Profile. The web version is accessible to health workers, as well as the research team and application developers who monitor and manage the user-conducted screenings. The average SUS score was 76 (standard deviation, 8.00). Conclusions This application was developed to help detect active tuberculosis cases in the community. The SUS results indicate that the application is highly usable. Thus, SIKRIBO is expected to be broadly implemented to increase tuberculosis case detection through active community participation.

Introduction: Tobacco production is increasing in certain regions of Indonesia due to its high economic value. Tobacco and its products can have harmful effects on the health of consumers, producers and processing workers. Analysis of blood samples has shown that tobacco farmers are at risk of developing occupational diseases related to pesticide exposure and nicotine absorption through the epidermis of wet tobacco leaves. The aim of this study was to compare blood cholinesterase levels in tobacco plantation workers in relation to their working hours and use of personal protective equipment (PPE). Methods: This cross-sectional observational study was conducted in ten subdistricts in Jember district, Indonesia, and included 50 participants selected using proportional sampling. Spearman’s test was used to analyse the relationship between variables. Results: The significance value of the relationship based on Spearman’s test between working hours and blood cholinesterase levels of tobacco farmers in Jember was 0.058 > α (0.05). Meanwhile, the significance value of the relationship between working hours and complete blood count, specifically mean corpuscular haemoglobin concentration (MCHC), was 0.00 < α (0.05), and between PPE use and blood cholinesterase levels was 0.035 < α (0.05). Conclusion: Working hours were not associated with blood cholinesterase levels, but were associated with blood MCHC levels. Meanwhile, the use of PPE was found to be associated with cholinesterase levels in tobacco farmers.