Introduction an Ulcer is defined as disruption of the mucosal integrity of the stomach and/or duodenum and is associated with increased risk factor for stomach and pancreatic cancer. Gastric ulcer incidence is increasing among young adults worldwide. Disability resulting from gastric ulcer is not declined. Due to the traditional Mongolian disease classification, gastric ulcer is equivalent to gastric ‘bor’. Stone-based medicines are widely used in Mongolian traditional medicine. Mineralbased medicines are safe to store and transport and there are relatively big sources of minerals comparing to animal and plant- originated raw materials. Calcite is the most widely used mineral among other stone-based medicines. Chronic diseases such as gastric ‘bor’ are treated by calcite containing medicines in Traditional Mongolian Medicine. Objectives In this study we examined the effect of calcite on animal model of gastric ulcer using biochemical analysis. Materials and methods All experiments were accomplished in the laboratory of the Scientific and Technological Corporation of Traditional Medicine according to the permission of Biomedical Ethics Review Committee of Ministry of Health. We used male calcite found in the cave named ‘Tsagaan agui’ which is located in the territory of ‘Bayanlig’ soum of ‘Bayanhongor’ province. In Traditional Mongolian Medicine, two different methods of drug subduing (detoxification and improving its delivery and effectiveness) are used; rich and strong. In this study, we subdued calcite by using both methods and the effect of rich subdued calcite was examined on acetic acid-induced gastric ulcer in experimental animal models. Strong subdued calcite was used for only determining its mineral compositions. Thirty healthy mice (body weight 23-40 g) and 30 Wistar rats (body weight 200-300 g) were used for the study. Average lethal dose of calcite was examined by the V. P. Prosorovsky’s express method (1978) and evaluated according to the classification of K. K. Sydorov (1987). Mineralogical study on calcite was performed by X-Ray Fluorescence Element Analyzer MESA-500W-HORIBA (MNS JIS K 0119:2003). Gastric ulcer was developed by acetic acid by the method of S. Okabe and J. Koth (1971). Healing process of the gastric ulcer was monitored by histochemical analysis. Results Calcite had turned bright yellow with bitter, strong and sour taste after the rich subduing. Moreover it became softer and changed its shape to small crystals without sharp ends and obtained dissolving property in water. According to the classification of Sydorov, average lethal dose of rich subdued calcite was 14.6 g/ kg suggesting that calcite exerts minimal toxic effects. Mineral examination results showed that some toxic elements such as Si, P, F, Mn, Al, and S were reduced in both calcites. Compositions of Na, Mg, and Ê were increased in rich subdued calcite. Compositions of Na, Mg, K, and Fe were decreased and Ca composition was increased in strong subdued calcite. Rich subdued calcite at concentration of 1 g/kg had anti-inflammatory effects reducing vascular permeability and improving blood circulation. Histological analysis showed that acetic acid-induced gastric ulcer begun to heal at 7th days after the calcite administration. Proliferation was determined at 14th days and the ulcer was completely disappeared at 21st days after the calcite administration. Conclusions 1. Calcite became softer and water soluble after rich subduing. 2. Calcite has minimal toxic effects according to Sydorov’s classification. 3. Mineral examination results showed that compositions of essential elements were increased and compositions of possible toxic elements were reduced in both subdued calcites. 4. Rich subdued calcite had anti-inflammatory effects reducing vascular permeability and improving blood circulation. 5. Acetic acid-induced gastric ulcer was treated by calcite within 21 days suggesting that calcite speeds up gastric ulcer healing.

Abstract The scientists established that in the present time, consumption of medication containing chemical agents in its compound is increased in 2.1 – 2.8% and consumption of medication made with natural raw material in 3.5 – 4.8%. However, reserve of medicines of natural origin herbs became very rare and issues on their protection and cultivation of them are discussed a lot. In contrary, raw materials of mineral origin medicaments that are widely used in drug treatment of traditional medicine have such advantages as having sufficient resources, require no high technology for their storage and transportation and they are rarely polluted and spoiled. On the basis of above background, we put goal for our research work to tame the spar and explore the mineralization composition contained in it. Male spar selected for our research work is crystal of age of 750 years with the sediment of Medium Paleozoic limestone from the cave “Tsagaan agui”, Bayanlig soum, Bayankhongor aimag. According to the traditional medicine spar is called as precious stone and bone elixir and it is function is to stop diarrhea, eliminate the cancer of stomach and fever, and joint the bones [1]. We tamed the selected spar in milk and alcohol according to the traditional method [2], and carried out the trial using the roentgenfluorescence tools HOBIRA- 500W. The present tools determines the quality and quantities of the chemical elements in the solid powder sample by the roentgenfluorescence method (MNS JIS K 0119:2003) according to the standard requirements creating x-ray in the tube with high voltage and burning and then determine the mineralization measuring the created fluorescence ray. The main component of untimed spar is CaCO3 and some mixtures of such elements as Si, FE, MN, Al, S, Na, Mg and they exist in the form of oxide or carbon oxide salt. After taming the spar by traditional methods it became small crystal no razor edge soft and its colour turns into withish yellow, taste became sour and spicy, odour was sour and with milk odour, and it became soluble in water. It is established that elements such as Si, P, F, Mn which may be harmful for human organism were reduced in two kinds of tamed spar and besides that content of Ca, a useful element was increased in the tamed and extracted spar and but useful elements such as Na, Mg and K were reduced in the strong tamed spar.

Introduction Medicinal plants and herbal drug Introduction Medicinal plants and herbal drugs have made and a tremendous contribution to national health and development from the very beginning. Fora very long time, people have used locally available medicinal plants for medicaltreatment and have eaten certain vegetables and spices for health protection as well as sustenance. Even though in traditional medicine, we use many different medicinal plants and herbal preparations for treatment of various diseases, most of them are not completely studied yet. One of these herbal preparations is Jonsh-6. The research group reported already that the preparation Jonsh-6 has pharmacological effects such as to treat diseases such as gastritis, stomach disorder (B.Uyanga, S.Seesregdorj, 2011) and at this time there aren’t any other studies made in respect to the preparation Jonsh-6. The aim of our research is to complete qualitical and quantitative analyses of biological active substance in the preparation Jonsh-6. Material and methods The contents of the preparation Jonsh-6 are Calcitum-0.3g, Piper longim L- 0.08g, Aucklanda lappa Decne-0,15g, Inula helenium L-0.15g, Carthamus tinctorius L-0.24g, Amomum Kravanh Pierreex Gagnep-0.08g.Analyses of the preparation were studied by using HPLC on Waters Delta 600 Pump, Waters 600 Controller, Waters 2487 DualχAbsorbance Detector, Pinnacle II C18 5μm, 250x4.6 ммand selected following conditions: mobile phase: Acetonitrile:water/50:50,Acetonitrile:0.7% phosphoric acid /25:75,wavelength 210, 338, 403 nm, Detector: UVvis, column: Pinnacle IIC18 5μm (250x4.6 мм), flow rate 1.0 ml/min. Key words: HPLC, Jonsh-6, Safflomin-A, Piperine, Alantolaktone, and Costunolide. Conclusion In order to succeed the above mentioned aim, we have achieved the following results: 3. The chromatographical method and methodology forthe qualitical and quantitative analyses of main compounds in the preparation Jonsh-6 were developed using HPLC. 4. The quantity of main components in the preparation Jonsh-6 were determined with HPLC and Safflomin-A 1.06 mg, piperine 0.195 mg, alantolaktone 1.12 mg and costunolide 0.925 mg are contained in 1.003 gm of the preparation.