1.Prevention of acute renal failure in obstructive jaundice patients in perioperative period
Dong DAI ; Xiaoyu TAN ; Xiancheng ZENG ; Songtao DU
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo investigate the protection of renal function and the prevention of acute renal failue(ARF) in perioperative period in obstructive jaundice(OJ) patients. Methods In experimantal group, short time parenteral nutrition(PN) was given preoperatirely.Small dosage of dopamine was intravenously dripped.and rapid infusion of 20% mannitol periodically was done.In control group routine treatment was done. Postoperative blood BUN and Cr dynamic changes were examined and occurrence rate of ARF were recorded. Results No ARF occurred in the experimental group;but ARF occurred in 5 cases(29.4%)and 2 cases(17.6%) died (P
2.Down-regulation of ObR by lentivirus-mediated RNA interference inhibits growth of MCF-7 cells xenograft in a nude mouse model
Rongquan XUE ; Junchao GU ; Songtao DU ; Wei YU ; Xianghou XIA ; Zhigang BAI ; Xuemei MA
International Journal of Surgery 2012;39(4):236-239
ObjectiveTo investigate the inhibitory effect of lentivirusly-mediated ObR-siRNA on transplanted MCF-7 human breast cancer cells by intratumoral injection.MethodsA model of subcutaneous implanted tumor was generated through injecting MCF-7 human breast cancer cells into the nude mice.Thirty established mice with MCF-7 breast cancer cells xenograft were divided into 3 groups randomly,and mice in the experimental group were intratumorally injected with ObR-siRNA lentivirus,while the negative control group and blank control group mice were injected with the same dose of negative lentivirus and normal saline.All mice were subcutaneously injected with recombinant human leptin around the tumor site once a day.Tumor size was blindly measured every other day and the mRNA expression and protein expression levels of ObR in each group were determined.ResultsKnockdown of ObR-treated xenografted nude mice with a high leptin microenvironment was successfully established.Local injection of ObR-siRNA lentivirus significantly suppressed the established tumor growth in nude mice(P < 0.01,P <0.01 ).Real time-PCR and Western blotting showed that the mRNA and protein expression of ObR was decreased in the ObR-siRNA lentivirus group( P < 0.01,P < 0.01 ).ConclusionsIntratumoral injection of recomhinant ObR-siRNA lentivirus inhibits the growth of MCF-7 cells xenografts in the nude mice,suggesting that ObR might represent a therapeutic target in the genotherapies of human breast cancer.
3.Behavioral study of internet gaming addiction in adolescents
Xiaodong LI ; Yongxin YANG ; Yunting ZHANG ; Quan ZHANG ; Linxiang LIU ; Guijin DU ; Songtao ZHAO ; Baotao LU
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(6):535-537
Objective To explore the facilitative mechanism of the behavioral improvement resulting from gaming experience in internet gaming addicts. Methods Seventeen gaming addicts and twenty normal subjects were involved in the study, and their behavioral performance were evaluated by using the revised paradigm of event-related design. Results 1. Mean reaction times (RTs) under valid and invalid cue conditions were ( (614.27 ± 85.25)ms,(645.4±80.24)ms)and ((710.24 ± 131.42)ms,(765.53 ± 142. 53)ms) respectively. There were statistically significant differences of mean RTs within each group (P < 0. 05 ) and between the two groups (P < 0.01 ). 2. There were statistically significant differences of mean RTs on three cue levels between addiction group (online game picture(643.65 ±90.82) ms,neutral face picture(616. 73 ±82. 19)ms,neutral picture(629. 21 ± 78.20)ms) and control group(game picture (744. 54 ± 140. 42) ms, neutral face picture ( 727. 98 ± 142.26)ms, neutral picture(741. 13 ±138. 21)ms, P<0.05).3. There were statistically significant differences on trends of mean RTs on three cue levels of addiction group between valid cue condition ( online game picture (619. 02 ± 91.00) ms,neutral face picture (604.90 ±90. 71 )ms,neutral picture (618.87 ±77.85)ms) and invalid cue con-dition( online game picture ( 668. 28 ± 86. 27 ) ms, neutral face picture ( 628. 56 ± 73. 53 ) ms, neutral picture (639.54 ±79. 54) ms, P<0. 05). Conclusion Both exogenous and endogenous attention orientation are improved in internet gaming addicts. Enhancement of endogenous attention function resulting from gaming experience might be greater than that of exogenous attention.
4.Effect of insulin pump in treatment of type two diabetic patients with pulmonary infections
Weimin ZHAO ; Xiulan ZOU ; Yingnan WANG ; Jianming YANG ; Yanqun LIU ; Ping YU ; Songtao DU
Chinese Journal of Postgraduates of Medicine 2006;0(27):-
Objective To study the effect of continuous subcutaneous insulin injection(CSII) in treatment of type two diabetic patients with pulmonary infections. Methods From January 2001 to December 2005,83 type two diabetic patients with pulmonary infections were randomized to group CSII (42 cases) and group MSII(41 cases). The period of normalization of the blood glucose and cure of the pulmonary infections, cost and morbidity of low blood glucose were observed. Results Blood glucose of all patients in two groups reduced significantly. Significant difference was observed in the period of cure of the pulmonary infections, usage amount of insulin and morbidity of low blood glucose. In group CSII HbA1c, CRP and CHOL reduced significantly. Fasting CRP and high density lipoprotein-C (HDL-C) were remarkably increased. Conclusions Consecutive subcutaneous insulin pump injection is able to control the blood glucose better, to correct metabolic disorder, reduce the cost and hospital day and decrease the morbidity of low blood glucose. CSII is a better of method.
5.Clinical effect of recombinant adenovirus containing thymidine kinase suicide gene in preventing postoperative recurrence of hepatocellular carcinoma
Jian MENG ; Songtao DU ; Jinguang ZHANG
Journal of Clinical Hepatology 2017;33(10):1944-1948
Objective To investigate the clinical effect of surgical operation combined with gene therapy in preventing postoperative recurrence of hepatocellular carcinoma (HCC).Methods A total of 102 patients with single HCC (TNM stage 1-2,tumor diameter < 10 cm) who were admitted to Beijing YouAn Hospital,Capital Medical University,from July 2006 to February 2013 were enrolled,and among these patients,60 underwent the gene therapy with recombinant adenovirus containing thymidine kinase suicide gene (ADV-TK) before and after surgery (gene group) and 42 underwent surgical resection alone (surgery group).The patients were followed up regularly after surgery to observe postoperative recurrence.The t-test was used for comparison of continuous data between groups,and the chi-square test was used for comparison of categorical data between groups.The log-rank test was used for the analysis of cumulative recurrencc rate,and univariate and multivariate Cox regression analyses were used to identify influencing factors for recurrence rate.Results The 1-,3-,and 5-year recurrence rates of tumor were 13.8%,33.7%,and 47.7% in the gene group and 18.5%,53.2%,and 69.2% in the surgery group,and there was a significant difference between the two groups (x2 =2.643,P =0.041).The gene group had a significantly higher proportion of patients with pyrexia after surgery than the surgery group,and there were no significant differences in the incidence rates of other complications and length of hospital stay between the two groups.The multivariate analysis showed that gene therapy was an independent influencing factor for cumulative rumulative recurrence rate (odds ratio =2.752,95 confidence interval:1.1 64-4.251,P =0.038).Conclusion Gene therapy conbined with surgical resection can effectively reduce postoperative recurrence of tumor,and therefore,it holds promise for clinical application.
6.Clinical analysis of thyroid carcinoma in 110 children and adolescents
Liyuan DAI ; Wei DU ; Shanting LIU ; Songtao ZHANG
Chinese Journal of Applied Clinical Pediatrics 2018;33(23):1792-1795
Objective To study the clinical and pathologic factors for prognosis of children and adolescents with thyroid carcinoma.Methods Clinicopathological data of 110 children and adolescents with thyroid carcinoma at the Affiliated Tumor Hospital of Zhengzhou University from January 1984 to March 2008 were collected and reviewed in this study.Univariate analysis was performed by using Log-rank test to determine predictors of survival,and multiva-riable analysis was performed by a Cox regression model.Results The overall 5- year and 10 -year survival rates were 95.5%,88.2%,respectively.Gender and histopathologic typing were not related to prognosis.The 5 -year and 10-year survival rates in group ≤12 years old were 91.7% and 81.3%,and those in group >12 years old were 98. 4% and 93.5%,respectively.The 5-year and 10-year survival rates in stage T1 and T2 were 100.0%,in stage T3 were 100.0% and 66.7%,respectively,and those in stage T4 were 58.3% and 0.The 5-year and 10-year sur-vival rates in N0 group and N1a group were 100.0%,and those in N1b group were 93.4% and 82.9%,respectively. For patients without the distant metastasis group,the 5-year and and 10-year survival rates were all 96.9%,which were significantly higher than those in the distant metastasis group(84.6%,23.1%).The 5-year and 10-year sur-vival rates in groupⅠwere all 97.8%,those in groupⅡwere 84.6% and 23.1%,respectively,and those in groupⅣwere all 75. 0%.The 5-year and 10-year survival rates in the solitary lesions group were 98.7%,in the multiple le-sions group were 88.2% and 64.7%,respectively.For differentiated thyroid carcinoma,the 5-year and 10-year sur-vival rates in low,and medium risk groups were all 100.0%and those in high risk group were 83.3% and 50.0%,re-spectively.There was a significant relationship between survival and age group,T stage,N stage,M stage,TNM stage, the number of lesions,and risk stratification(all P<0.05).The Cox regression analysis showed that T stage and M stage were the independent prognostic factors.Conclusions The primary tumor and distant metastatic are important factors affecting the prognosis of children and adolescents.Earlier diagnosis for primary tumor and aggressive manage-ments for metastatic lesions are critical to the survival rate of children and adolescents with thyroid carcinoma.
7.Characterization methods of flowability of traditional Chinese medicine powders.
Yan DU ; Lijie ZHAO ; Yi FENG ; Desheng XU ; Xiaohai LI ; Songtao WANG
China Journal of Chinese Materia Medica 2012;37(5):589-593
OBJECTIVETo characterize the flowability of traditional Chinese medicine (TCM) powders by using appropriate methods.
METHODWith highly flowable direct compression excipients and Chinese traditional medicine powder as raw materials, the flowability of material powders is determined by the Carr method and the Jenike method.
RESULTThe Carr method and the Jenike method for the determination of the flowability of Chinese herb extract powder and direct-pressing excipients have no obvious difference. But the flowability of Chinese herb extract powder is not as good as direct compression excipients.
CONCLUSIONFrom the characterization results of both methods, we can find that the Carr method better reflects the actual flowability of TCM extracts, while the Jenike method can be used for guiding the design of the hopper device.
Medicine, Chinese Traditional ; Particle Size ; Powders ; chemistry
8.Digital assisted chimeric deep circumflex iliac artery perforator flap in the reconstruction of mandibular composite defects
Zhaojun ZHAN ; Shengkai LIAO ; Yongfeng CHEN ; Tingyi GAO ; Xiaoying DU ; Liang LIU ; Dongkun YANG ; Songtao YU
Chinese Journal of Microsurgery 2019;42(5):429-433
To evaluate the clinical effect of digital assisted chimeric deep circumflex iliac artery perforator flap (DCIAPF) in the reconstruction of mandibular composite defects. Methods From January, 2018 to January, 2019, 6 cases of mandibular tumor patients with postoperative defect within side were treated. Preoperative CTA was used to evaluate the deep branches of spin iliac artery.Digital simulation software and 3D printing technolo-gy was taken, vascularized iliac flap of the design guide of bone was made, and the rebuilding effect was simulated. DCIAPF was used to repair the defect of lower jawbone. The donor sites were sutured directly. The patients were fol-lowed-up in outpatient department for 3-6 months to evaluate the recovery of the patient′s shape, jaw height and oc-clusal function, as well as the complications in the donor area. Results Postoperation pathological examination re-sults: ameloblastoma in 2 cases, 4 cases of gingival cancer. The length of cut out ilium was 6.0-13.0 cm, carrying the flap area of 3.0 cm×1.0 cm-6.0 cm×5.0 cm.Six cases of DCIAPF and iliac bone flap survived.The shape, mandibular height and occlusal function were satisfactory.And no obvious complications were found in the donor area. Conclu-sion The blood supply of DCIAPF is rich with enough bone mass and height. The position of terminal skin perfora-tors is invariant. The complications of donor sites is less. With the help of digital technology, the accuracy of mandibular defect repair and the 3-dimensional wound repair can be realized, and provides an advantage condition for subsequent dental implant.It is one of the ideal method of reconstruction of mandibular defect.
9.Impact factors analysis on child neglect of children aged 3-6 year-old in rural areas of China.
Fei WANG ; Jianping PAN ; Songjie ZHANG ; Hua ZHANG ; Weiqing WANG ; Fangbiao TAO ; Rui QIN ; Guixiong GU ; Shuhua SHI ; Jing ZHANG ; Qiyun DU ; Zhaohui ZHONG ; Feng JIAO ; Huishan WANG ; Tao XU ; Guixiang WANG ; Weiping XI ; Songtao PANG ; Xin WANG ; Chunhong CAO ; Min LI ; Xiaomei YANG
Chinese Journal of Preventive Medicine 2015;49(10):866-872
OBJECTIVETo investigate the major influence factors of child neglect among rural areas children aged 3-6 years in China.
METHODSAccording to multi-stage stratified cluster sampling, distribution characteristics and the level of economic development, we randomly sampled 10 provinces, 26 cities (8 capital cities, 16 prefecture-level cities and 2 municipalities) using lottery method. Depending on the different level of economic and cultural, we sampled one medium county from each city (municipalities sampled two counties), a total of 28 counties. All towns were divided into high, medium and low three levels in accordance with economic and cultural, each level sampled one town, each conty sampled three towns, a total of 84 towns were sampled. Each town sampled 40 children, including 20 boys and 20 girls (including each 10 children aged 3 to 6, half and half boys and girls). 4 096 rural children aged 3-6 years old were sampled and surveyed of China. Based on "the Neglect Norms for Children Aged 3 to 6 Years Old in Rural Areas of China" explored the risk factors of child neglect. SPSS 18. 0 for windows was employed for statistics analysis. Multifactorial analysis was conducted through multivariate Logistic Regression.
RESULTSThe total neglected rate of the rural children aged 3 to 6 was 53.7% (2,047/3,810), and the total neglected degree was 44.42±7.57. The multivariate logistic regression analysis showed that, neglected risk of the boys was higher than the girls (OR=1.30, 95% CI: 1.13-1.49), neglected risk of children were higher, when their mothers were migrant workers (OR=2.18, 95% CI: 1.59-3.00); the lower educational level of mothers, the higher neglected risk of children, the children in families that mothers only received primary education or was uneducated were most likely to be neglected (OR=2.92, 95% CI: 1.91-4.47), the second was the families that mothers received primary education (OR=1.86, 95% CI: 1.29-2.69); the lower the household income, the easier to be neglected the children, the easiest one was the household income less than 5000 RMB (OR=2.85, 95% CI: 2.14-3.79), the second was 5000-9,999 RMB (OR=1.76, 95% CI: 1.40-2.20); the relationship between children and mothers was distanced, neglect risk of children was higher (OR=3.88, 95% CI: 1.31-11.52); left behind children were easier to be neglected (OR=1.30, 95% CI: 1.09-1.54).
CONCLUSIONThe influence factors among children aged 3-6 years in the rural areas of China were boys, mother's occupation, maternal education level, the relationship between children and mothers, household income and left-behind children.
Child Abuse ; Child, Preschool ; China ; Demography ; Factor Analysis, Statistical ; Female ; Humans ; Male ; Mothers ; Risk Factors ; Rural Population ; Surveys and Questionnaires ; Transients and Migrants