A comparative experiment with the biological half-life of ~(125)I-labe- lled human serum albumin prepara- tions,produced by two kinds of te- chnological process,was performed in rabbits.The biological half-life of two human serum albumin prepa- rations in rabbits,obtained from de- termination and linear logarithmic equation calculation,was 69?26 hours and 85?15 hours respectively. The survival time of every labelled human serum albumin preparation in two groups of rabbits was above 10 days.Result of the experiment show- ed that the intrinsic quality and stability of the human serum albumin serum albumin preparation,produced by the two different technological processes,were in agreement.

This article briefly discusses the business process of hospitalization management in constructing the digital hospital and also respectively expounds the optimization to the process of medicine,inspection,examination and operation in implementing the new system.At last,it analyzes some insufficiencies and suggestions to the new system in business process optimization.

Objective To compare the morphology and ultrastructure changes of human gastric adenocarcinoma cell line treated by tachyplesin and n-sodium butyrate. Methods Light microscope,scanning electron microscope and transmission electron microscope were used to observe human gastric adenocarcinoma cell line BGC-823 treated by 2.0?mg/L tachyplesin,2.0?mmol/L n-sodium butyrate and 1.0?mg/L tachyplesin in combination with 1.0?mmol/L n-sodium butyrate respectively. Results Light microscope observation showed that BGC-823 cells treated by tachyplesin,n-sodium butyrate and tachyplesin+n-sodium butyrate possessed the similar cell modality as follow: the volume of cell increased,the shape of cell became flat and outspread,nucleo-cytoplasmic ratio decreased,the shape of nucleus was rounde,the number of nucleolus decreased.Scanning electron microscope and transmission electron microscope observation showed that,in the BGC-823 cells which were treated with tachyplesin,n-sodium butyrate and tachyplesin in combination with n-sodium butyrate respectively,microvilli and filopodia reduced,sheed pseudopodia increased,the shape of nucleus became regular,heterochromatin decreased while euchromatin increased,the number of mitochondria increased and its structure appeared consistent,Golgi complex turned to be typical,the amount of rough endoplasmic reticulum increased and that of polyribosome decreased.Conclusion All of these results showed that tachyplesin possessed the similar effects to n-sodium butyrate on changing morphology and ultrastructure of human gastric adenocarcinoma cells and possessed an additive role of inducing tumor cells to differentiate cooperatively with n-sodium butyrate to induce carcinoma cell differentiation.

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Pomalidomide,which represents a new generation of immunomodulatory drugs (IMiD),has brought a major shift in therapeutic paradigm in treating relapsed and refractory multiple myeloma (RRMM).Considering its encouraging effectiveness,the U.S.Food and Drug Administration (FDA) approved its usage to treat patients who fail to respond to at least two prior therapies,including lenalidomide and bortezomib,and whose disease progressed within 60 days of the last treatment.This review summarizes the mechanisms of action and clinical activity of pomalidomide in treating RRMM.

BACKGROUND: The mouse model of cervical heart transplantation is an ideal medical research tool for study of transplant-induced ischemia/reperfusion injury and immunological rejection.However,technical problems have limited the widespread use of mouse cervical vascularized heart transplantation.OBJECTIVE: To improve the cervical heterotopic heart transplantation in mice using the tail-cuff technique.METHODS: Isogeneic transplantation was performed from Balb/c to BALB/c mice,and allogeneic transplantation from C57BL/6 to BALB/c mice.The right common carotid artery and the external jugular vein of the recipient were equipped with a tail cuff made from 24 G and 22 G intravenous catheter,and everted over the cuff,and then connected with the aorta and the pulonary artery of donor heart,respectively.RESULTS AND CONCLUSION: A total of 36 transplants for formal experiment,12 for isogeneic transplantation,and 24 for allogeneic transplantation,were performed with a surgical successful rate of 100%.The total surgical procedure was(49.6±7.4)minutes and total ischemic time of the grafts was(28.8±4.2)minutes.In particular,the average time for vascular everting and for the reconnection of both vessels was obviously shortened.This improved tail-cuff technique shows its superiority,and can serve as an ideal method for establishing cervical heterotopic heart transplantation model in mice.

BACKGROUND: Studies have demonstrated that vascular endothelial cell growth factor (VEGF) and basic fibroblast growth factor (bFGF) play important roles in entochondrostosis and fracture healing blood vessel hyoperplasia. OBJECTIVE: To observe and compare the roles of VEGF and bFGF in fracture healing. DESIGN, TIME AND SETTING: Double-factor trial was performed at Pathological Laboratory, North China Coal Medical University from August 2005 to May 2006. MATERIALS: Twenty-four adult healthy Japan rabbits were selected and radial fracture models were created in 48 bilateral anterior limbs. The rabbits were randomly divided into VEGF group and bFGF group (n=24). METHODS: VEGF (0.2 ?g) and bFGF (100 ng) were injected respectively in the fracture site of rabbits. No external fixation was used. MAIN OUTCOME MEASURES: The rabbits were executed at postoperative weeks 2, 4 and 6 to measure callus sagittal diameter, transverse diameter and section area. X-ray was used to observe fracture healing and measure bony callus total area. Histological alterations during fracture healing were observed, and percentage of trabecular bone, cartilage and fibrous tissue in the bony callus was determined. RESULTS: At 2 weeks after operation, callus sagittal diameter and transverse diameter in VEGF group were significantly larger than bFGF group (P

Chemical characteristics of callosal neurons in the frontal and occipital cortex of Wistar rat were studied by means of combined method of HRP retrograde tracing andimmunocytochemistry of glutamate (Glu) and ?-aminobutyric acid (GABA). Glutamate containing callosal neurons were large or medium sized pyramidal cells and mainly localized in layers Ⅱ/Ⅲ, Ⅴ and Ⅵ. They tended to appear in clusters. GABA containing callosal neurons were also found. They were medium sized nonpyramidal cells with round, elliptic, or fusiform soma and were mostly localized in layers Ⅴ and Ⅵ. They also tended to appear in clusters. The percentage of GABA containing callosal neurons (about 8% and 10% in frontal and occipital cortex respectively)was much less than that of glutamate containing callosal neurons (about 17% and 29% in frontal and occipital cortex respectively). These results further confirmed that part of callosal cells used glutamate as an excitatory transmitter and firstly confirmed that some callosal neurons contained an inhibitory transmitter GABA morphologically. In view of the existence of GABA containing callosal neurons, it also suggested that at least in rat cerebral cortex,some GABA containing neurons had longer projections than local circuit neurons. According to the results of ours and other authors, we suggested that callosal system was the one that contained different kinds of neurotransmitters, and that diversification of the transmitters and their interaction in the callosal system was one of the neuroanatomical bases of the complicated and advanced functions of the callosal neurons.

Object To study the technological conditions of refining ?-linolenic acid by short-path distillation (SPD). Methods According to the characteristic of SPD, the effect of the operating parameters, such as the distillation temperature, the system pressure, the feeding rate, and the agitating speed, were studied on the purity of ?-linolenic acid. Results The appropriate process conditions for the purification of ?-linolenic acid by SPD are: distillation temperature 90 ℃—120 ℃, pressure 0.3 Pa, feeding temperature 60 ℃, feeding rate 90—100 mL/h, and rotating speed 150 r/min. Conclusion Refining ?-linolenic acid by SPD is a simple and convenient method. It is easy to achieve industrialization.

Interleukin-32 is a newly descovered cytokine. In vivo and in vitro studies show that it inhibits the proliferation and induces apoptosis of colon cancer and melanoma by inhibiting NF-κB and signaling and transcription activated factor 3 pathways.In addition,IL-32 suppresses tumor growth in different ways in chronic myelogenous leukemia,sarcoma and prostate cancer.