1.Determination of Biological Half-Life of ~(125)(?)-labelled Human Serum Albumin in Rabbits
Chinese Journal of Blood Transfusion 1988;0(03):-
A comparative experiment with the biological half-life of ~(125)I-labe- lled human serum albumin prepara- tions,produced by two kinds of te- chnological process,was performed in rabbits.The biological half-life of two human serum albumin prepa- rations in rabbits,obtained from de- termination and linear logarithmic equation calculation,was 69?26 hours and 85?15 hours respectively. The survival time of every labelled human serum albumin preparation in two groups of rabbits was above 10 days.Result of the experiment show- ed that the intrinsic quality and stability of the human serum albumin serum albumin preparation,produced by the two different technological processes,were in agreement.
2.Research progress of pomalidomide in the treatment of relapsed refractory multiple myeloma
Journal of Leukemia & Lymphoma 2014;23(3):179-181
Pomalidomide,which represents a new generation of immunomodulatory drugs (IMiD),has brought a major shift in therapeutic paradigm in treating relapsed and refractory multiple myeloma (RRMM).Considering its encouraging effectiveness,the U.S.Food and Drug Administration (FDA) approved its usage to treat patients who fail to respond to at least two prior therapies,including lenalidomide and bortezomib,and whose disease progressed within 60 days of the last treatment.This review summarizes the mechanisms of action and clinical activity of pomalidomide in treating RRMM.
3.EFFECTS OF TACHYPLESIN AND N-SODIUM BUTYRATE ON THE MORPHOLOGY AND ULTRASTRUCTURE CHANGES OF HUMAN GASTRIC ADENOCARCINOMA CELLS LINE BGC-823
Acta Anatomica Sinica 1955;0(03):-
Objective To compare the morphology and ultrastructure changes of human gastric adenocarcinoma cell line treated by tachyplesin and n-sodium butyrate. Methods Light microscope,scanning electron microscope and transmission electron microscope were used to observe human gastric adenocarcinoma cell line BGC-823 treated by 2.0?mg/L tachyplesin,2.0?mmol/L n-sodium butyrate and 1.0?mg/L tachyplesin in combination with 1.0?mmol/L n-sodium butyrate respectively. Results Light microscope observation showed that BGC-823 cells treated by tachyplesin,n-sodium butyrate and tachyplesin+n-sodium butyrate possessed the similar cell modality as follow: the volume of cell increased,the shape of cell became flat and outspread,nucleo-cytoplasmic ratio decreased,the shape of nucleus was rounde,the number of nucleolus decreased.Scanning electron microscope and transmission electron microscope observation showed that,in the BGC-823 cells which were treated with tachyplesin,n-sodium butyrate and tachyplesin in combination with n-sodium butyrate respectively,microvilli and filopodia reduced,sheed pseudopodia increased,the shape of nucleus became regular,heterochromatin decreased while euchromatin increased,the number of mitochondria increased and its structure appeared consistent,Golgi complex turned to be typical,the amount of rough endoplasmic reticulum increased and that of polyribosome decreased.Conclusion All of these results showed that tachyplesin possessed the similar effects to n-sodium butyrate on changing morphology and ultrastructure of human gastric adenocarcinoma cells and possessed an additive role of inducing tumor cells to differentiate cooperatively with n-sodium butyrate to induce carcinoma cell differentiation.
4.Optimization of Hospitalization Business Process in Digital Hospital
Chinese Medical Equipment Journal 2003;0(10):-
This article briefly discusses the business process of hospitalization management in constructing the digital hospital and also respectively expounds the optimization to the process of medicine,inspection,examination and operation in implementing the new system.At last,it analyzes some insufficiencies and suggestions to the new system in business process optimization.
6.Technology of refining ?-linolenic acid in Semen Sesami oil by short path distillation
Chinese Traditional and Herbal Drugs 1994;0(03):-
Object To study the technological conditions of refining ?-linolenic acid by short-path distillation (SPD). Methods According to the characteristic of SPD, the effect of the operating parameters, such as the distillation temperature, the system pressure, the feeding rate, and the agitating speed, were studied on the purity of ?-linolenic acid. Results The appropriate process conditions for the purification of ?-linolenic acid by SPD are: distillation temperature 90 ℃—120 ℃, pressure 0.3 Pa, feeding temperature 60 ℃, feeding rate 90—100 mL/h, and rotating speed 150 r/min. Conclusion Refining ?-linolenic acid by SPD is a simple and convenient method. It is easy to achieve industrialization.
7.Molecular basis for losartan regulating transient outward potassium current
Chinese Journal of Geriatrics 2010;29(5):424-428
Objective To investigate the molecular basis for anti-ventricular arrhythmic effects by losartan through measuring alteration in mRNA and protein levels of key K+ α channel-and-β subunits (Kv4.2,Kv4.3 and KChIP2) in ventricular myocytes in spontaneously hypertensive rats (SHRs).Methods SHRs were randomly assigned to losartan[10mg·kg-1·d-1,n=12] or placebo (n= 12) with age-and weight-matched WKY rats (n = 12) as control.After 8 weeks of treatment,cardiomyocytes were isolated by enzymolysis.Action potential of cardiomyocytes Ito was recorded,mRNA and protein levels of Kv4.2,Kv4.3 and KChIP2 were assessed by reverse transcriptase polymerase chain reaction and Western blot.Results The action potential duration (APD) measured at 50% and 90% repolarization was shorter in losartan group [(16.82 ± 3.79) ms and (68.49±13.25) ms] than in SHR control group [(24.56±4.59) ms and (73.26±15.47) ms,all P<0.01].Losartan increased Ito current density associated with significant increases in the mean levels of mRNA and protein of Kv4.2 and Ky4.3,and with significant decreases in the mean levels of mRNA and protein of KChIP2 compared with those in placebo SHR (all P<0.01).Conclusions Chronic blockade of AT1 receptors with losartan reverses cardiomyocytes electrical remodeling in SHR,resulting in the shortening of APD,which is associated with increasing Ito density by increasing mRNA and protein expression of Kv4.2,Kv4.3 and by decreasing mRNA and protein expression of KChIP2.
8.Influence of vascular endothelial growth factor versus basic fibroblast growth factor on radial fracture healing in rabbits
Chinese Journal of Tissue Engineering Research 2007;0(33):-
BACKGROUND: Studies have demonstrated that vascular endothelial cell growth factor (VEGF) and basic fibroblast growth factor (bFGF) play important roles in entochondrostosis and fracture healing blood vessel hyoperplasia. OBJECTIVE: To observe and compare the roles of VEGF and bFGF in fracture healing. DESIGN, TIME AND SETTING: Double-factor trial was performed at Pathological Laboratory, North China Coal Medical University from August 2005 to May 2006. MATERIALS: Twenty-four adult healthy Japan rabbits were selected and radial fracture models were created in 48 bilateral anterior limbs. The rabbits were randomly divided into VEGF group and bFGF group (n=24). METHODS: VEGF (0.2 ?g) and bFGF (100 ng) were injected respectively in the fracture site of rabbits. No external fixation was used. MAIN OUTCOME MEASURES: The rabbits were executed at postoperative weeks 2, 4 and 6 to measure callus sagittal diameter, transverse diameter and section area. X-ray was used to observe fracture healing and measure bony callus total area. Histological alterations during fracture healing were observed, and percentage of trabecular bone, cartilage and fibrous tissue in the bony callus was determined. RESULTS: At 2 weeks after operation, callus sagittal diameter and transverse diameter in VEGF group were significantly larger than bFGF group (P
9.Meta-analysis of the effectiveness of laser therapy in the treatment of dentin hypersensitivity in China
Journal of Practical Stomatology 1995;0(04):-
Objective: To evaluate the effectiveness of laser therapy in the treatment of dentin hypersensitivity in China.Methods: The Cochrane reviewer's handbook was followed.Domestic published articles from 1989~2009 were selected by computer and handsearch.All these retrieved study were clinical control trials related to laser therapy in the treatment of dentin hypersensitivity.The quality of included studies was critically evaluated and data were analyzed by the RevMan 5.0.1 software.Results: A tota1 of 114 articles were selected,but only 9 were included.The results showed that: although the data of immediate efficiency of laser treating dentin hypersensitivity could be combined,significant heterogeneity was found when the other studies' data were pooled.So the data could not be merged,only the descriptive analysis could be used.The immediate efficiency for the treatment of dentine hypersensitivity using lasers was higher than the control group,the difference was statistically significant[OR=3.83,95%CI(2.86,5.12)],other period of efficacy had a trend which was superior to the control group.Conclusion: Comparing with the control group,the desensitizing of dentin hypersensitivity with laser is more effective.However,because the literature quality of this study is not high,the above conclusion needs to be verified by larger sample-size,rigorously designed,randomized controlled clinical trials.
10.Effect of losartan on ventricular arrhythmia in elderly patients with hypertension
Chinese Journal of Geriatrics 2011;30(1):24-26
ObjectiveTo evaluate the effect of losartan on ventricular arrhythmia in elderly patients with hypertension.Methods The 78 hypertension subjects with ventricular arrhythmia aged 65 to 89 years were randomly assigned to treatment with losartan or placebo (enalapril) for 12 months. The blood pressure, left ventricular mass index and ventricular arrhythmia were observed and analyzed.ResultsAfter 12 months treatment, the pressure lowering effect was similar in losartan versus enalapril groups, there were no differences in systolic pressure decrement and diastolic pressure decrement between the two groups (P>0. 05). Left ventricular mass index was lower in enalapril groupthaninlosartangroup [(109.2±15. 4) g · m-2 vs. (128.5±16. 7) g · m-2, t=2.015, P<0. 05].However, the prevalence of ventricular arrhythmia was lower in losartan group than in enalapril group [(628. 5±176.8)/24 h vs. (852.9±215.7)/24 h, t=2.417,P<0.05]. No Pearson's correlations of reduction of ventricular arrhythmia with reduction of blood pressure (systolic: r=0. 094, P>0. 05; diastolic: r= 0.08, P>0. 05) and reduction of left ventricular mass index were found. in losartan group.Conclusions Initiation of antihypertensive treatment with losartan in elderly subjects appears to cause more reduction of ventricular arrhythmia than with enalapril, despite similar reduction of blood pressure.