Objective To discuss the preventive measures of anterior palatal fistula by modified the operation of cleft palate. Methods For 23 patients of complete cleft palate with alveolar ridge cleft, bilateral mucoperiosteum flap was moved forward as possible so as to close fistula front of hard palate, and incised junction of the hard and soft palate to prolong soft palate and the wound was repaired by buccal mucosal flap.The incidence of anterior palatal fistula and velopharyngeal closure after operation was observed. Results Twenty-three patients were rechecked 1 month after operation ,there was no anterior palatal fistula occurring,10 cases were examined by epipharyngoscope 1 year after operation,the velopharyngeal closure was 90%-100%. Six cases were followed up for 6 months,the velopharyngeal closure was 80%-85%. Conclusions For second-stage operation methods of anterior palatal fistula, there are too many discussions of selection criterias, advantages and disadvantages. If first-stage operation is taken measures to prevent anterior palatal fistula or decrease the diameter of fistula as possible. It reduces percentage of second-stage operation or decreases the difficulty. It should get more attention in the clinical works.

Objective: 1.To analyze the nutritional composition and evaluation of the nutritional value of Mortierella isabelina powder. 2.To investigate the effects of Mortierella isabelina powder on prevention and regulation of hyperlipidemia in rats. Methods: 1. Mycelium powder was analyzed for proteins, lipids polysaccharides, fibre, ash, vitamin E, amino acids, fatty acids and minerals. 2. Mycelium powder was used to feed rats in different dosages together with high lipid diet for 10 days. 3. Mycelium powder was used to feed hyperlipidemic rats in different dosages for 30 days. Results: (1) There were about 20% of proteins and more than 50% of lipids in the mycelium. Further examination found that the mycelium contained rich essential amino acids and unsaturated fatty acids. The mycelium also contained vitamin E and useful minerals such as K, Ca, P, Fe, Zn and Mn. (2) 0.6 or 1.2 g/(kg?d) dosage of mycelium powder could prevent the rise of serum lipids. (3) When the mycelium powder was used to feed hyperlipidemic rats, the serum cholesterol, triglyceride, LDL C and VLDL C of the rats could be reduced, meanwhile HDL C could be increased. Conclusion: The results indicated that Mortierella isabelina powder has potential nutritional value and can be used to regulate the blood lipids in hyperlipidemic rats.

Objective To investigate the expression and clinical significance of platelet activating factor [PAC]-1, CD62P and TPP hi severe sepsis. Method Patients with severe sepsis who were admitted into the EICU of Subei People's Hospital from April 2007 to March 2008 were included. Patients with severe sepsis (Group Ⅲ)were treated according to the treatment guidelines for severe sepsis, and were divided, according to their clinical records, into those who survived and those who died within 28 days of admission. Patients admitted during the same period with symptoms of infection but without severe sepsis were included as the General Infected Group (Group Ⅱ). A Control Group (Group Ⅰ) comprised patients who visited the hospital over the same period for physical examination or the healthy volunteers. The group members were all included randomly, and the gender and sex of patients in all three groups were similar. Patients with acute brain infarction, acute coronary syndrome,serious diabetes, hyperlipidemia, malignant tumor, leukemia, primary liver, renal and hematopoietic system dis-eases,long-term bedridden patients, pregnant women, and patients taking hormone treatment or hranunosuppres-sants were excluded from the study. Morning venous blood was collected and ELISA and Flow Cytometry performed on the fwst day of admission for Groups Ⅰ- and Ⅱ, and on the first, third and fifth day after admission for Group Ⅲ, to determine the TpP,PAC-1 and CD62P respectively; and the Marshall score was determined. Data were ana-lyzed by SPSS 12.0 software. For continuous variables, comparisons among groups were analyzed by ANOVA.Levene's and LSD test were applied to assess homogeneity. Bivariate test is applied to Correlation Analysis. P<0.05 was regarded as a statistically significant difference. Results There were a total of 20 patients each in GroupⅠ-and GroupⅡ, and 30 in Group Ⅲ; of these, 19 were classed as survivors and 11 died during the 28-day peri-od. On the first day of admission, there were no significant differences in PAC-1, CD62P or TpP expression between Groups Ⅰ- and Ⅱ(P>0.05); however, Group Ⅲ was significantly different compared with both Group Ⅰ and Group Ⅱ (both:P<0.05). The expression of PAC-1, CD62P and TpP tended to decline in the survivor group,and became normal with the treatment process, while the expression of PAC-1 ,CD62P and TpP in the patients who died remained high, and even increased significantly over time. On the first day, the expression of CD62P and TpP in the patients who survived and in those who died was not significantly different (P>0.05); on the third day,however, a significant difference appeared with values of (2.89±1.48) % vs. (5.04±2.57) % (P<0.01) for CD62P, and (5.24±2.22) mg/L vs. (9.20±1.93) mg/L (P<0.01) for TpP. The expression of PAC-1 was significantly different between the two subgroups on the first day, with values of (3.15±0.42)% vs. (5.30±.48)% (P<0.01). The Marshall score of the two groups showed similar changes. Correlation analysis showed that PAC-1, CD62P and TpP were significantly correlated with the Marshall score. Conclusions Platelet activation and microthrombosis existing in the early stage of severe sepsis work together in the early hypercoagulable state.They both play important roles in disease development and progression. The dynamic detection of CD62P and TpP is beneficial to the diagnosis and prognosis of severe sepsis.PAC-1 appears to hold a risk stratification effect, as pa-tients with high expression of PAC-1 in the early stage show poor prognosis. Therefore, PAC-1 could be used as a marker of severe sepsis and poor prognsis.

Objective To investigate the infection rate, subtypes and distribution characteristics of human papilloma virus (HPV) women in Yangzhou. Methods A retrospective analysis was conducted on 4 682 female patients treated at Northern Jiangsu People's Hospital from May to September 2018. The fluorogenic quantitative PCR was used to detect high-risk HPV typing in cervical exfoliated cells and the results were statistically analyzed. Results A total of 936 positive samples were screened out in 4 682 subjects, the positive rate was 19. 99％, among whom 718 cases with single infection (76. 71％) and 218 with multiple infection (23. 29％) were included. The common subtypes of high-risk HPV infection were HPV 52, 16, 58 and 51, with the positive rate of 25. 00％ (234/936), 20. 73％ (194/936), 16. 67％ (156/936) and 9. 94％ (93/936), respectively. The infection rates of women in different age groups showed significant differences (P ＜ 0. 05), and the higher positive rates distributed in age groups in descending sequence were as follows: 61 ～ 70 years old (32. 00％), 51 ～ 60 years old (26. 32％) and ≤20 years old (21. 43％). Conclusion The main HPV infection is single infection in Yangzhou. Common HPV subtypes are 52, 16, 58, 51, 39, and the patients among age groups have differed HPV subtypes.

Objective To investigate the infection rate, subtypes and distribution characteristics of human papilloma virus (HPV) women in Yangzhou. Methods A retrospective analysis was conducted on 4 682 female patients treated at Northern Jiangsu People's Hospital from May to September 2018. The fluorogenic quantitative PCR was used to detect high-risk HPV typing in cervical exfoliated cells and the results were statistically analyzed. Results A total of 936 positive samples were screened out in 4 682 subjects, the positive rate was 19. 99％, among whom 718 cases with single infection (76. 71％) and 218 with multiple infection (23. 29％) were included. The common subtypes of high-risk HPV infection were HPV 52, 16, 58 and 51, with the positive rate of 25. 00％ (234/936), 20. 73％ (194/936), 16. 67％ (156/936) and 9. 94％ (93/936), respectively. The infection rates of women in different age groups showed significant differences (P ＜ 0. 05), and the higher positive rates distributed in age groups in descending sequence were as follows: 61 ～ 70 years old (32. 00％), 51 ～ 60 years old (26. 32％) and ≤20 years old (21. 43％). Conclusion The main HPV infection is single infection in Yangzhou. Common HPV subtypes are 52, 16, 58, 51, 39, and the patients among age groups have differed HPV subtypes.