Objective To demonstrate the feasibility, effectiveness and reliability of combination of procedure for prolapse and hemorrhoids (PPH) and excision-closed hemorrhoidectomy (ECH) for severe hemorrhoids. Methods Sixty patients with Ⅲ- Ⅳ degree hemorrhoids which left remnant prolapsed hemorrhoids after stapled hemorrhoidopexy, were randomly divided into group A (PPH with additional ECH group,30 cases.) and group B [PPH with additional Milligan-Morgan hemorrhoidectomy (MMH) group, 30 cases]. Ten indexes of the results in two groups were compared. ResultsThe hemorrhoidectomy lasted (35.6 ± 5.3) min in group A and (47.3 ± 10.2) min in group B. The indexes related to 24 h visual analogue scale (VAS) after operation were (2.9 ± 1.2) scores in group A and (5.7 ± 1.9) scores in group B. Wound healing time were (6.3 ± 1.4) d in group A and (28.5 ± 4.8) d in group B. Many indexes showed the results in group A was better than those in group B ( P ＜ 0.05 ). The six-month follow-up visits after operation showed no complications, such as recurrence in either group. ConclusionsCombination treatment for Ⅲ -Ⅳ degree hemorrhoids with PPH with additional ECH is a easy,safe,reliable,minimal-aching and quick-recovering method of hemorrhoidectomy with favorable results than PPH with additional MMH.

Objective To determine the content of calycosin-7-?-O-D-glucopyranpside in Danggui Buxie Dropping Pills.Methods HPLC method was used. The chromatographic column was YMC-C18(150?4.6 mm, 5 ?m), the mobile phase was methanol and water(40 ∶60), the detection wavelenth was 235 nm, column temperature at 20 ℃and flow rate was 1.0mL/min.Results Calycosin-7-?-O-D-glucopyranpside was separated well with other components and was in good linearity in the range of 0.02～0.80 ?g. The average recovery was 97.62 %and RSD=1.06 %.Conclusion The method is rapid and accurate and can be used for quality control of Danggui Buxie Dropping Pills.

Breast cancer is a heterogeneous disease.Its invasion and metastasis,recurrence and part of the chemotherapy drug tolerance are currentlv difficulties of treatment.Breast cancer stem cells have the ability of selfrenewal,proliferation and differentiation potential,which may correlate closely with breast cancer recurrence,treatment failure.This assay aims to briefly analyse markers and significance of breast cancer stem cells,and the relationship between the treatment of breast cancer and breast cancer stem cells.

To find out the patterns and trend of clinical use of antihypertensive agents to provide a guidance for rational use of drugs, the antihypertensive drugs used in our hospital during 1995-1999 period was investigated in respect to the sum of consumption cost, drugs used and DDDs. Our results showed that the DDDs and the consumption cost for antihypertensive drugs have increased gradually for recent 5 years. The order of DDDs of drugs was as follows: calcium antagonists, adrenergic blocking drugs, ACE inhibitors, the drugs affecting sympathetic transmitters and peripheral vasodilators. The order of sum of money was as follows: ACE inhibitors, calcium antagonists and calcium antagonists respectively. It is concluded that a diverse array of anthhypertensive agents were available and widely used clinically and attention should be paid to the rational use of the drugs.

Objective To established the method of increasihg the proportion of T regulatory cells(Tr) to T effector cells(Te), which could suppresses allogeneic ahtigen reaction, by in vivo of glucocorti-coid (dexamethasone, DXM) combined with IL-2. Methods After combined treatment to male C57BL/6N mice (donor) with DXM(5 mg. kg-1· d-1 ) and IL-2 (300 000 IU · mouse-1·d-1) for 3 d, spleen mono-nuclear cells were made and were carried out by flow cytometry analysis. Using the spleen cells of BALB/c mice as aliogeneic antigen to stimulate the spleen cells of male C57BL/6N mice for 7 d after combined treat-ment of glucoeorticoid and IL-2, the reaction of cell proliferation was detected. Results After the treatrment of DXM and IL-2, CD4+ CD25+ Foxp3+ Tr cells in the spleen of C57BL/6N mice increased abviously. The ratio of CD25+ Foxp3+ Tr to CD4+ T cells was 24.22%±7.60% in the group of DXM combined with IL-2, while the control group was 4.02% ±0. 84% ( P =0. 01 ). Compared with the control group (0. 14±0.01 ), the ratio of Tr to Te increased obviously in the group of DXM combined with IL-2 (0.43±0. 15 ) ( P = 0.01 ). The group of DXM combined with IL-2 also expressed more glucocorticoid-induced tumor necrosis factor receptor(GITR) and alloreaction was suppressed/n vitro obviously ( P < 0. 05 ). Conclusion DXM amplifies IL-2 induced the proportion of Tr to Te, and suppresses the cell proliferation stimulated by alloge-neic antigen.

Objective:To prepare resveratrol microemulsions by high pressure homogenization method and characterize the microe-mulsions. Methods:Using the particle size, polydispersion index and encapsulation efficiency as the indicators, the independent varia-bles of the preparation were inspected, and the microemulsions were characterized. The stability of resveratrol microemulsions was stud-ied by long term stability test preliminarily. Results:The mean particle size, polydispersion index and zeta potential of resveratrol mi-croemulsions was (231 ± 37. 8) nm, 0. 228 ± 0. 047 and ( -42. 5 ± 4. 3) mV, respectively. The microemulsions were found to be small and spherical with smooth surface under a transmission electron microscope. Long term stability studies showed that the microe-mulsions were stable in 3 months after stored at 25℃. Conclusion:The preparation process of high pressure homogenization method for resveratrol microemulsions is simple and feasible.

Objective:To evaluate the significance of expression of laminin (LN) and vascular endothelial growth factor(VEGF) in adenoid cystic carcinoma of salivary glands. Methods:Ultrasensitive immunohistochemical staining(S-P) was used to examine LN and VEGF expression in specimens from 34 patients with adenoid cystic carcinoma of salivary glands.Results:Higher LN expression was found in adenoid or tubular type of the cases (P0.05). Higher VEGF expression was found in stage Ⅲ-Ⅳ of the tumor (P0.05). Conclusion: LN and VEGF expression may be served as one of the predictors for the biological behavior of adenoid cystic carcinoma of salivary glands.

Objective To examine the changes of the airway resistance(AR) in asthmatic bronchitis children before and after budeso-nide-solution inhaled therapy.Methods Fifty-six cases of asthmatic bronchitis children were randomly divided into A(regular treatment+budesonide-solution inhaled) and B groups(regular treatment),and 30 normal children at the same age as control group.The AR was eva-luated by Microloop lung function meter with MicoroRint sensor.The changes of AR were compared within above 3 groups.Results AR in asthmatic bronchitis children increased significantly compared with normal children.After 2 weeks treatment,AR decreased significantly in both A and B groups compared with that of 2 groups before therapy,AR in A groups declined to normal control level,but still kept higher level in B groups.Conclusions AR in asthmatic bronchitis children increase significantly.The effect of Budesonide-solution in asthmatic bronchitis children is partly via reducing AR to improve ventilated condition.

Retinal neural degeneration diseases is one of the main causes of vision loss , TNF-αas an inflammatory cytokine with pleiotropic biological activity , after binding to its receptor by a variety of signaling pathways , may have play critical role in pathogenesis of retinal neural degeneration diseases including glaucoma , ischemic retinop-athy, age-related macular degeneration and retinitis pigmentosa .

BACKGROUND:Nano-hydroxyapatite/collagen (NHAC) is an ideal substitute for tissue engineered stent. Vascular endothelial growth factor (VEGF) is a kind of growth factor that can promote vascularization and slowly release NHAC. This indicated a good bone tissue repair function. OBJECTIVE:To investigate the effect of NHAC combined with VEGF in repairing bone defects. DESIGN,TIME AND SETTING:The randomized controlled animal study was performed at the Animal Laboratory,Xiangya Medical College,Central South University from October 2008 to January 2009. MATERIALS:Nano-hydroxyapatite powder and collagen protein powder were mixed and prepared into NHAC artificial bone according to the ratio of 8:2. 2 ?g of VEGF and NHAC were mixed and prepared into NHAC/VEGF artificial bone according to the ratio of 1.8:1. Each artificial bone contained 0.5 g nano-hydroxyapatite powder,0.125 g collagen protein and 150 ng VEGF. METHODS:Thirty-six healthy rabbits whose left radiuses were cut off 1 cm were divided into three groups. The wounds of the rabbits in blank group were sewed up directly without any treatment of the bone. The bone defects of the rabbits in NHAC group were repaired with NHAC artificial bones and the NHAC/VEGF group with NHAC/VEGF artificial bones. MAIN OUTCOME MEASURES:After 2,4 and 8 weeks,4 rabbits in each group were killed to evaluate whether they have significant differences of bone healing according to general observation,radiograph,pathological section and bone density test. RESULTS:Radiograph showed that osteotylus growth and the repairing of bone defects in the NHAC/VEGF group were better than in the blank group and NHAC group. It was better in the NHAC group than in the blank group. The difference in bone density of two groups in the same time was significant (P