Objective To investigate the correlation of smokers in wnt/β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.Methods In the Department of Respiratory Medicine of the People's Hospital of Wuhan University,30 cases lung squamous cell carcinoma patients with a history of smoking,30 cases lung squamous cell carcinoma patients with no history of smoking,30 cases bronchiectasis patients with a history of smoking,30 cases bronchiectasis patients with no history of smoking were chose respectively as A group,B group,C group and D group.After the patients were treated by surgery,the removal of diseased tissue used immunohistochemical staining for CD68 + testing,TNF-α,wnt-2,β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method,then wnt-2,β-catenin,TCF-4 transcription of mRNA concentrations were detected by using RT-PCR method.Results Immunohistochemical studies showed that cells in TNF-α,CD68 + expression of A group,B group,C group and D group were respectively 0.895±0.036 and 0.931±0.237,0.125±0.018 and 0.631±0.064,0.083±0.042 and 0.311±0.062,0.053±0.004 and 0.196±0.020,the differences were statistically significant(P＜0.05).Westblot test Results showed that wnt-2,β-catenin,TCF-4 expression level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05),testing of RT-PCR showed that wnt-2,β-catenin,TCF-4 translation level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05).Conclusion Smoking for body wnt/β-catenin pathway has a cell activation,after cigarette activate wnt/β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt/β-catenin signaling transduction circumstances.The detection of wnt/beta-catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.

Objective: To observe the effect of acupuncture around the diseased region combined with point injection on post-herpetic neuralgia. Methods: Point injection on 30 cases in the related segments of spinal cord after needling around the diseased region. Results: Twenty-seven cases got effect and the total effect rate was 90%. Conclusion: Acupuncture combined with point injection has remarkable curative effect on post-herpetic neuralgia.

Objective To explore the role of plasma level of homocysteine ( Hey ) in progressive ischemic stroke (PIS)of carotid artery system and to investigate the relationship between Hcy and carotid artery atherosclerosis(AS).Methods Plasma Hcy concentrations were measured in 68 patients with progressive ischemic stroke of carotid artery system (PIS group)and 72 patients with non-progressive ischemic stroke of carotid artery system ( non-PIS group ).Plasma Hcy level and carotid artery intima-media thickness ( IMT ) were compared among PIS group and non-PIS group as well as normal control group.The linear correlation between plasma Hcy level and IMT in PIS group was analyzed.Results Plasma Hcy concentrations was respectively (22.52 ± 5.26) μmol/L,( 16.38 ± 4.23) μmol/L and( 10.13 ± 2.16) μmol/L in PIS group,non-PIS group and normal control group( F =56.87,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant ( P ＜ 0.01 ).And there was also significant difference on plasma Hcy levels between PIS group and non-PIS group( P ＜ 0.01 ).The thickness of carotid artery IMT was respectively(1.12 ± 0.34)mm,(0.96 ± 0.27)mm and(0.43 ± 0.21 )mm in PIS group,non-PIS group and normal control group( F =28.47,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant( P ＜ 0.01 ).And there was also significant difference on IMT between PIS group and non-PIS group ( P ＜ 0.01 ).In PIS group plasma Hcy level was positively correlated with the carotid artery IMT ( r =0.687,P ＜ 0.01 ).Conclusion The plasma Hcy level is closely related to the occurrence and development of carotid artery atherosclerosis and PIS of carotid artery system,detection of plasma Hcy level helps to predict the occurrence of PIS of carotid artery system and provide an evidence for the therapy decision.

Objective To explore the mechanism of the acute cerebral infarction leading to systemic inflammatory response syndrome (SIRS) which causes multiple organ dysfunction syndrome (MODS), and to explore the role of tumor necrosis factor (TNF-?) and IL-1? in the mechanism from SIRS to MODS.Methods 68 cases of acute cerebral infarction (ACI) were divided into three groups. They were 36 cases of simple acute cerebral infarction (SACI group), 32 cases accompanied with SIRS (SIRS group) and 24 cases accompanied with MODS (MODS group). Enzyme linked immunosorbent assay (ELISA) method was used to measure serum TNF-? and IL-1? contents at different time after ACI and compared with 28 healthy persons.Results (1) 47.06% of the 68 cases were accompanied with SIRS, of whom 75.00% were accompanied with MODS.(2) The serum TNF-? and IL-1? levels were the highest in MODS group, next in SIRS group, then in SACI group, the lowest in control group. The differences were significant among 4 groups (all P

When applied to the inspection of the organophosphorus and carbamate pesticides, acetylcholinesterase has a high sensibility and a high activity. The sensibility is related to its activity, selectivity, amount, temperature and so on. With acetylcholinesterase liquor prepared, this paper discusses the relationship between the concentration of organophosphorus pesticide and its inhibition effect on acetylcholinesterase, and the relationship between different concentrations, temperatures of acetylcholinesterase and the inhibition ability. The experiment data present the theory basis of the practical application of acetylcholinesterase to the inspection of the pesticide.

Acute lung injury is the result of organism autodestruction due to the mediators of inflammation overexpress. Recent studies suggest that GM-CSF correlate with alveolar macrophage function, lung host defense reaction and pulmonary surfactant homeostasis. GM-CSF influences regulation and signal transduction pathway of inflammation and apoptosis. These research progresses was been reviewed.

Retrospective analyses were performed for the clinical data of 67 patients with biliary calculi.Among them,laparocholedochoscopy plus holmium laser lithotripsy (n =31) and laparocholedochoscopy (n =36) were performed.As compared with laparocholedochoscopy,early postoperative serum inflammatory mediators of holmium laser lithotripsy were obviously lower,biliary pressure dropped faster and less volatile[(8.5±1.5)-(12.2±4.1) vs.(8.1 ±1.6)-(16.5±4.7) mmHg(1 mmHg=0.133 kPa)],operative duration [(93.5 ± 13.4) vs.(127.6 ± 34.5) min],conversion into laparotomy rate[3％ (1/31) vs.11％ (4/36)],postoperation SIRS rate[23％ (7/31) vs.42％ (15/36)],length of hospital stay [(8.5 ± 3.5) vs.(12.6 ± 3.9) days] and postoperative biliary residual stones rate [0％ (0/31) vs.8％ (3/36)]were also better than.

Objective To investigate the relationship between p38MAPK and astrocytes activation by detecting the changes of p38MAPK,TNF-?,NO and NOS in the substance P-induced activation of spinal cord astrocytes in vitro.Methods Astrocytes,cultured from spinal cord of SPF rat and identified by immunofluorescence method with GFAP antibody,were grouped into the control group,the SP stimulus group(SP group) and the SB203580 interrupt p38MAPK group(interrupt group) in which SP(10-7 mol/L) and SB203580(10 ?mol/L) were added to the supernatant for 12 h.The WB method,RT-PCR and ELISA were used to determine the changes of p38MAPK,phosphorylated p38MAPK,GFAP mRNA,TNF-?,NO and NOS in the astrocytes or supernatant at 1,3,12,24 and 36 h respectively.Results The GFAP positive rate of the cultured cells was higher than 95%.p38MAPK level in the SP group did not have any change,whereas p-p38 level increased significantly after 1 h and GFAP mRNA,NO,NOS and TNF-? level increased after 3 h.When p38MAPK pathway was inhibited by SB203580 in the SP+SB group,GFAP mRNA,NO,NOS and TNF-? were significantly reduced compared with those in the SP group.The level of p38MAPK,p-p38,GFAP mRNA,NO,NOS and TNF-? in the SB group did not have changes compared with those in the normal group.Conclusion These results indicate that p38MAPK signal pathway contributes to substance P induced activation of spinal cord astrocytes according to inflammatory factors attenuation after p38MAPK signal pathway interruption by SB203580.

Objective To investigate the expression of p38 mitogen-activated protein kinase(p38MAPK) in posterior horn of L5-S2 spinal cord in analgesia for chronic prostate pain with intrathecal injection of SB203580(p38MAPK inhibitor activity) in a murine model.Methods Forty-five male SPF SD rats were randomly divided into control group(n=5),prostatitis group(n=10) and SB203580 analgesia group(n=30,SB203580 was intrathecally injected).In the SB203580 analgesia group,complete Freund's adjuvant was injected into the prostate and SB203580 of 0.5?g/10?l,2.5?g/10?l and 12.5?g/10?l was intrathecally injected respectively.Five days and ten days after drug administration or simple modeling,phosphorylated p38MAPK(p-p38MAPK),glial fibrillary acidic protein(GFAP),tumor necrosis factor-?(TNF-?),nitric oxide(NO) level and induced nitric oxide synthase(iNOS) activity of L5-S2 spinal dorsal horn of rats were determined by Western blotting and ELISA assay,respectively.Results In the prostatitis group,the p-p38MAPK,GFAP,TNF-?,NO level and iNOS activity of L5-S2 spinal dorsal horn of rats at the 5th day and the 10th day were significantly higher than those of the control group and increased with time.The p-p38MAPK,GFAP,TNF-?,NO levels and iNOS activity at the 5th day and 10th day in SB203580 analgesia group were lower than those in the prostatitis group.Conclusion P38MAPK signaling pathway was involved in the mechanism of pain conduction and analgesia by SB203580 after prostatitis.The level of inflammatory factors in spinal cord can be effectively reduced by blocking the p38MAPK signaling pathway.

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.