Objective To explore the role of plasma level of homocysteine ( Hey ) in progressive ischemic stroke (PIS)of carotid artery system and to investigate the relationship between Hcy and carotid artery atherosclerosis(AS).Methods Plasma Hcy concentrations were measured in 68 patients with progressive ischemic stroke of carotid artery system (PIS group)and 72 patients with non-progressive ischemic stroke of carotid artery system ( non-PIS group ).Plasma Hcy level and carotid artery intima-media thickness ( IMT ) were compared among PIS group and non-PIS group as well as normal control group.The linear correlation between plasma Hcy level and IMT in PIS group was analyzed.Results Plasma Hcy concentrations was respectively (22.52 ± 5.26) μmol/L,( 16.38 ± 4.23) μmol/L and( 10.13 ± 2.16) μmol/L in PIS group,non-PIS group and normal control group( F =56.87,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant ( P ＜ 0.01 ).And there was also significant difference on plasma Hcy levels between PIS group and non-PIS group( P ＜ 0.01 ).The thickness of carotid artery IMT was respectively(1.12 ± 0.34)mm,(0.96 ± 0.27)mm and(0.43 ± 0.21 )mm in PIS group,non-PIS group and normal control group( F =28.47,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant( P ＜ 0.01 ).And there was also significant difference on IMT between PIS group and non-PIS group ( P ＜ 0.01 ).In PIS group plasma Hcy level was positively correlated with the carotid artery IMT ( r =0.687,P ＜ 0.01 ).Conclusion The plasma Hcy level is closely related to the occurrence and development of carotid artery atherosclerosis and PIS of carotid artery system,detection of plasma Hcy level helps to predict the occurrence of PIS of carotid artery system and provide an evidence for the therapy decision.

Objective To investigate the correlation of smokers in wnt/β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.Methods In the Department of Respiratory Medicine of the People's Hospital of Wuhan University,30 cases lung squamous cell carcinoma patients with a history of smoking,30 cases lung squamous cell carcinoma patients with no history of smoking,30 cases bronchiectasis patients with a history of smoking,30 cases bronchiectasis patients with no history of smoking were chose respectively as A group,B group,C group and D group.After the patients were treated by surgery,the removal of diseased tissue used immunohistochemical staining for CD68 + testing,TNF-α,wnt-2,β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method,then wnt-2,β-catenin,TCF-4 transcription of mRNA concentrations were detected by using RT-PCR method.Results Immunohistochemical studies showed that cells in TNF-α,CD68 + expression of A group,B group,C group and D group were respectively 0.895±0.036 and 0.931±0.237,0.125±0.018 and 0.631±0.064,0.083±0.042 and 0.311±0.062,0.053±0.004 and 0.196±0.020,the differences were statistically significant(P＜0.05).Westblot test Results showed that wnt-2,β-catenin,TCF-4 expression level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05),testing of RT-PCR showed that wnt-2,β-catenin,TCF-4 translation level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05).Conclusion Smoking for body wnt/β-catenin pathway has a cell activation,after cigarette activate wnt/β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt/β-catenin signaling transduction circumstances.The detection of wnt/beta-catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.

Objective: To observe the effect of acupuncture around the diseased region combined with point injection on post-herpetic neuralgia. Methods: Point injection on 30 cases in the related segments of spinal cord after needling around the diseased region. Results: Twenty-seven cases got effect and the total effect rate was 90%. Conclusion: Acupuncture combined with point injection has remarkable curative effect on post-herpetic neuralgia.

Inflammatory reaction plays an important role in the occurrence and development of ischemic stroke. Monocyte chemotactic protein-1 (MCP-1) is one of the subfamily members of the CC chemokine class. It can attract and activate a variety of cells, such as monocytes and T lymphocytes, promote the expression of cytokines, and participate in the occurrence of ischemic brain injury. This article reviews the progresses on the MCP-1 and ischemic stroke-related research.

Objective To observe the effect of medicine-induced posterior vitreous detachment (PVD) on proliferative vitreoretinopathy (PVR).Methods.PVR was induced in the left eyes of 24 pigmented rabbits by intravitreal injection with platelet rich plasma.The rabbits were randomly divided into two experimental groups (group A and B) and one control group with 8 eyes in each group.Three hours later,the eyes in group A and B and the control group underwent intravireal injection with 1 U plasmin 0.05 ml+ 20 U hyaluronidase 0.05 ml,plasmin 0.1 ml,and balance salt solution 0.1 ml,respectively.The grade of PVR was recorded 1,7,and 28 days after the intravitreal injection,and the eyes were examined by flash electroretinogram (FERG),B-scan,and retinal histopathological examination.Results The PVR models of rabbit eyes were induced successfully.On the 7th day after injection,complete and partial PVD was found in 5 and 3 eyes respectively in group A;partial PVD in 5 eyes and no complete PVD was observed in group B;there was no PVD in the other 3 eyes in group B and also in the eyes in the control group.On the 28th day after intravitreal injection,PVR grade of group A and B were both obviously lower than that of the control group(D= 75.6,98.9;P = 0.003,P = 0.011) ;On the 7th and 28th day after injection,the b-wave amplitude in group A and B was significantly higher than that in the control group;PVR grade of the PVD eyes was lower than that of non-PVD eyes;PVR grade of the complete PVD eyes was only 0 ～ 1.Conclusions Three hours after the PVR models of rabbit eyes were induced,complete PVD induced by intravitreal injection of plasmin combined with hyaluronidase could prevent the development of PVR of rabbit eyes in some degree;partial PVD induced by plasmin alone or combined with hyaluronidase could relieve the development of PVR.

Objeetive To explore a new therapeutic way of transurethral drugs perfusion combined with ultrashort wave on treating chronic bacteria prostatitis.Methods 241 cases with chronic bacteria prostatltis were randomly divided into two groups.the treatment group were treated with transurethral perfusion combined with ultrashort wave.the control group were treated with transurcthral drugs perfusion.Results Total effective rate in the treatment group was 85.9%.that of the control group was 36.7%.It had a significant difference between two groups(P＜0.01).Conclusion Transurethral drugs perfusion combined with ultrashort wave could promote the curative effect of antibiotic maximumly,so it is an effective method to treat chronic bacterial prostatitis.

Objective To study the role of serum C-reactive protein (CRP) ,supplement C3 and CA in sys-temic inflammatory response syndrome (SIRS) induced by acute cerebral infarction(ACI).Methods The levels of CRP,C3 and CA were measured by automatic immunoturbidimetry in 52 ACI cases accompanied with SIRS (SIRS group) ,50 cases of acute simple cerebral infarction(SACI group) and 46 normal cases (control group).Results The serum CRP, C3 ,and CA levels were significantly different among the three groups( F=720.6,580.8,94.1 ,P <0.01) ,the serum CRP levels in SIRS group were significantly higher than those in SACI group(P <0.01 ).The ser-um C3 and CA levels in SIRS were significantly lower than those in SACI group(P <0.01).With the diagnosis crite-rion items increasing, the serum CRP levels tended to be higher (t = 7.69 ,P < 0.01 ), and the serum C3, CA levels tended to be lower meeting SIRS diagnosis criterion numbers increasing respectively (t=10.68 and 10.53, P <0.01).Conclusion The serum ClIP levels are higher significantly and the serum levels of C3 and C4 are lower sig-nificantly when ACI progresses to SIRS.The serum CRP,C3,CA level changes can be used as an index for judging the progress of ACI progressed to SIRS.

Objective To investigate the levels of CD4 + CD25 + regulatory T cells and the Foxp3 in the peripheral blood of patients with ulcerative colitis ( UC), and analyze its role in the pathogenesis of UC. Methods From February 2007 to December 2008, 40 UC patients (23 active and 17 remissive), 33 irritable bowel syndrome (IBS) patients, and 32 normal controls entered our study. CD4 + CD25 + T cells were detected with flow cytometric assay. The expression of Foxp3 mRNA in peripheral blood mononuclear cell (PBMC) was detected by RTPCR. Interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) in the serum from the peripheral blood of UC patients, IBS patients, and normal controls were determined with ELISA. Results There were no significant differences of the peripheral CD4 +T cell numbers among the active, remissive UC and IBS patients, and normal control groups (P=0. 126). The positive rate of CD4+ CD25+ T cells in patients with active and remissive UC were significantly lower than those in IBS patients and normal controls ( both P ＜ 0. 01 ) it was more lower in the active UC patients than the remissive UC patients ( P ＜ 0. 001 ). However, the positive rate of CD4 + CD25 + T cells showed no significant difference between IBS patients and normal control groups ( P = 0. 343 ). The percentage of CD4 + CD25 + T cells was negatively correlated with UC disease activity index ( r = - 0. 660, P ＜ 0. 001 ) and with erythrocyte sedimentation rate (r = -0. 572, P =0. 001 ). The expressions of Foxp3 mRNA in PBMC from active and remissive UC patients were significantly lower than those in both IBS patients and normal controls ( both P ＜0. 001 ). There was no significant difference of the plasma levels of IL-10 or TGF-β among the active/remissive UC patients, IBS patients, and normal controls ( all P ＞ 0. 05 ). Conclusions CD4+ CD25 + regulatory T cells remarkably decline in the active UC and show certain increase in remissive UC, indicating that these cells may be involved in the pathogenesis of UC. The decreased expression of Foxp3 mRNA may be an important factor of the aberrant developmental disorder of CD4 + CD25 + regulatory T cells.

Objective To discuss the prenatal diagnosis of abnormalities of fetal limb bone.Methods We selected 18 cases which long bone of fetus less than 2SD of average volume of gestational weeks or long bone changed into angle or other fetus's abnormalities by first B-mode ultrasonic. All above cases was delivered at Capital Medical University of Obstetric and Gynecological Hospital during Jan. 2006 to Dec. 2009. We B-mode ultrasonic was used to measure fetus's biparietal diameter (BPD) ,femur length (FL) ,abdominal circumference(AC) ,head circumference(HC) ,humerus length (HL) ,amniotic fluid index (AFI) and structures of organ and calculated FL/AC, growth speed of long bone. The standard of achondroplasia is that FL and HL are less than 4SD of average of gestational weeks or FL/AC less than 0. 16. The standard of Osteogenesis Imperfecta is fetal long bone of fetus shows short and thick, curves into angle, fracture in uterus by X-ray, or skull shows thin or sink by X-ray. Results (1) By B-mode ultrasonic and X-ray exam of all 18 cases: 7 cases shows that HC ＞2SD, 10 cases shows too much amniotic fluid, 12 cases shows AFI ＞ 18.0, 9 cases shows abnormalities of narrow cavitas thoracis, disordered vertebral column, or unusual architecture of heart. For cases 1 to 14 are achondroplasia, among which,11 cases are FL＜4SD and HL＜4SD, 2 cases are FL ＜3SD and HL ＜4SD, 1 case is not only FL ＜2SD and HL ＜3SD but also hydroncus all over the body of fetus. The growth velocity of long bone of fetus in all the 14 cases is more slowly than the normal rate. For all the above 14 cases, 12 cases FL/AC ＜0. 16, 1 case FL/AC =0. 19, 1 case FL/AC =0. 20. The length of femur or humerus is shorter than the normal rate and have other abnormalities the above last two cases. For case 15 and 16, they don't show any abnormalities of bone growth though one year's follow up studying. For case 17 and 18, they are osteogenesis imperfecta. (2) The result of fetal perinatal period fate and autopsy: there are 8 female and 10 male in all the 18 cases. One case is labored after 39 weeks pregnancy, and it is low birth weight infant, weight ＜3%th. All the other cases are normally birth weight infant. All the 18 cases of abnormalities of fetal limb bone are examined by chromosomes check, among which, 9 cases are amniocentesis, 7 cases are cordocentesis,2 cases are checked chromosomes by fetus cord blood, all the caryotype are normal. In the 16 autopsy cases,14 cases are achondroplasia or hypochondroplasis. It can be seen amplifying extremities, hyperplasia chondrocytes of tubiform born, karyomegaly, anachromasis, hyperplasia capillaries though microscope and grow up into cartilage irregularly. Also can be seen hyperplasia chondrocytes of epiphyses, delaying osteosis.2 cases are osteogenesis imperfecta. It can be seen broadening of metaphyses, exility of bone trabeculae. For the other two cases which the fetus is alive, we do the follow up studying to their one year old one of them is low birth weight new born, their limb and height are all normal. Conclusions To diagnose fetal Achondroplasia, it is not only based on the significantly shorter of femur or humerus length but also based on the dynamics observing the long bone growth velocity and calculating FL/AC. For osteogenesis imperfecta fetus, it should be diagnosed by fractures in uterus though X-ray.

Objective To compare effect of the GlideScope and direct laryngoscopy in snoring surgery.Methods Fourty patients scheduled for orotracheal intubation of general anesthesia in snoring surgery wererandomly divided into GlideScope group ( A group) and direct laryngoscope group ( B group). Two groups were recorded values of heart rate (HR),systolic blood pressure (SBP), diastolic blood pressure (DBP)before induction (T1), after induction (T2), intubation (T3) and 1 (T4), 3 (T5), 5 (T6) min after intubation and plasma norepinephrine (NE) concentrations in T1, T5, T6. Results ( 1 ) Values of HR, SBP and DBP of two groups were compared in T1 and T2, the difference was not significant ( P ＞ 0.05 ); Values of SBP and DBP of two groups compared T1 to T2, and the difference was significant ( P ＜ 0. 05); (2) Values of HR, SBP and DBP of two groups compared in T3, T4 and T5, and the difference was statistically significant (P ＜0.05);(3 ) Values of the concentration of plasma NE of two groups compared in T1 ,T5 and T6, and the difference was statistically significant ( P ＜ 0. 05 ). Conclusion Cardiovascular response and stress response of orotracheal intubation using a videolaryngoscope is lower than using ordinary laryngoscope in snoring surgery.