[Objective] To screen the anticancer fraction from extract of root of Adina rubella Hance.[Methods]To separate the water extract from root of Adina rubella Hance by different solvens,then determine their anticancer activities of the different extracts in vitro with MTT method.[Results]The ethylacetate extract strongly inhibited cell proliferation of LS174T,and showed significant dosage-dependent response while the butanol extract and the chloroform extract showed low anticancer activities in vitro.[Conclusion]The ethylacetate extract is the principal anticancer extract from root of Adina rubella Hance.

Object To study the chemical constituent and the bioactivity in the bark of Tsoongiodendron odorum Chun Methods By bioactive following method, the extracts of both EtOAc and n BuOH in the bark of T odorum were screened for in vitro anti tumor activity Results Five constituents were obtained Among them, three from EtOAc fraction belonged to germacranolides They were costunolide (Ⅰ), parthenolide (Ⅱ) and dihydroparthenolide (Ⅲ) The other two were from the fraction of n BuOH, one was an oxoaporphinoid alkaloid, liriodenine (Ⅳ), and the last was a furanone, 2, 3 dihydroxyl 2 methyl butylrolactone (Ⅴ) Conclusion All the above five compounds are found for the first time from this plant Compounds Ⅰ, Ⅱ, Ⅳ, and Ⅴ show the cytotoxic activities against a variety of tumor cell strains, respectively

Adolescent ; Cardiomyopathies ; complications ; genetics ; Female ; Heart Ventricles ; abnormalities ; Humans ; Pedigree ; Sick Sinus Syndrome ; complications ; genetics

Objective To investigate the effects of different ligation position,vena coronaria variation and lidocaine on myocardial infarction size and mortality in rats Methods Totally 120 male SD rats were divided into four groups with 30 rats in each group: low ligation with invisible vena coronaria,low ligation with visible vena coronaria,high ligation,and high ligation plus lidocaine Results MI was successfully created in 930% rats of high ligation groups and in 673% rats of low ligation groups The mortality was 433% in rats of high ligation groups,only 133% in rats when lidocaine in use Conclusion High ligation increases the success rate of rat model of myocardial infarction and the application of lidocaine decreases the mortality of the rat model

Objective To investigate the effect of antisense telomerase reverse transcriptase (TERT) on 5-hydroxytryptamine (5-HT) induced proliferation and cell cycle of pulmonary artery smooth muscle cells (PASMCs). Methods PASMCs were cultured and divided into four groups: control group (cultured in RPMI-1640 culture medium), 5-HT group (cultured in culture medium containing 5-HT), antisense oligonucleotide (ASODN) group (cultured in culture medium containing 5-HT and ASODN TERT), and sense oligonucleotide (SODN) group (cultured in culture medium containing 5-HT and SODN TERT). The apoptosis of PASMC was observed by fluorescence microscopy with Hoechst staining. Apoptosis and cell cycle was analyzed with flow cytometry. Expression of proliferated cell nuclear antigen (PCNA) was determined by immunohistochemistry staining. Results Hoechst staining showed that apoptosis in 5-HT group (161?33) was significantly higher than that in control group (63?16, P

The residual crown and root has very important physiological significances,but there are many problems in the clinical treatment,such as overtreatment and defensive treatment,etc.This article analyze the background and causes of these problems,and provids some corresponding ways to solve them.

As an exposing part of the human body,facial surface is easy to be injured in our daily lives.With the increase of living standards,the patients have an ever more urgent require for aesthetic treatment for the medical and plastic surgical treatment for the exposing parts of body,especially for the facial surface.But we found in the clinical work some aesthetic treatment were not satisfying,and some patients even needed a second restitution.We have analyzed the reasons for this phenomenon and proposed some corresponding solving methods.