This study was aimed to investigate the dose-response relations and synergy effects of bioactive components in Ginkgo bilobaon antiplatelet aggregation and DPPH free radical scavenging.The antiplatelet aggregation experiment and DPPH free radical scavenging experiment were conducted respectively to investigate the dose-response relations and synergy effects.Firstly,the effect of inhibiting platelet aggregation induced by PAF of ginkgolides in rabbits was investigated.And then,the effect of DPPH free radical scavenging by ginkgo flavonoids was also investigated.Finally,the synergy effects among effective components were studied.The results showed that ginkgolide A,ginkgolide B,ginkgolide C and bilobalide had dose-response relations on antiplatelet aggregation.Quercetin,kaempferol and isorhamnetin had DPPH free radical scavenging effects with dose-response relation.It was concluded that ginkgolide had the dose-response relation on antiplatelet aggregation.Ginkgolide A and ginkgolide B had synergy effect.Ginkgo flavonoids had DPPH free radical scavenging effect.Quercetin and isorhamnetin had synergy effect.

As a novel T cell population,Th17 cells are demonstrated to play an important role in autoimmune diseases,including Crohn' s disease.Several recent studies indicate the specific contributions of IL-23/Th17 axis and cytokines secreted by Th17 cells to the inflammatory bowel disease.This article is dedicated to describe the correlation between Th17 cells and Crohn' s disease.

Impaired recognition of foreign nucleic acid initiates abnormal mucosal immune responses in intestine.Very little is known on how microbial DNA activates intracellular DNA sensors and controls the function of antigen-presenting cells to shape mucosal immune responses in inflammatory bowel disease (IBD).In current review,we will introduce mucosal dendritic cell population,describe newly identified cGAS-cGAMP-STING complex,and identify certain IBD susceptibility genes that associate with impaired microbial DNA recognition in human.

Objective To examine the blood lead levels and influencing factors among infants living in urban areas in Taiyuan.Methods A total of 216 healthy infants living in urban areas in Taiyuan who came to seek routine care during 2010 and 2012 were enrolled using questionnaire survey.Blood samples were collected and lead was meas-ured according to standard procedures.Results The total blood lead level was (31.54 ±22.24)μg/L,there was no significant difference between male and female.The infants whose blood level was less than 50 μg/L took the biggest proportion(80.56%),the infants whose blood level was among 50 -99μg/L and whose blood level more than 100μg/L were 19.44% and 0.00% respectively.Coal to cook,mother often catching hair,using prickly heat,sucking on the hand,washing hands before meals,living in storefront and playing time in roadside were associated with infantile blood lead level.Conclusion The key influencing factors the of the blood lead levels of infants are infantile living environ-ment and the keeper's awareness of preventing lead.

Objective To investigate the application value of prescription examination in the management of antimicrobial agents in hospital.Methods The prescriptions of antibacterials were examined,the irrational use of drugs was counted,and the application value of drug prescription examination in the management of antibacterial drugs in hospital was discussed.Results A total of 488 960 prescriptions were examined,including 133 534 prescriptions for antimicrobial agents,and 27.31％ for antimicrobial agents.Unqualified antibiotics use prescription in 60 086,unqualified rate was 45.00％,among them not standard prescription in 108,occupied 0.18％,medication unsuitable prescription in 59 907,occupied 99.70％,exceed prescription in 71,occupied 0.12％.Conclusion There is an irrational use of antibiotics in outpatient service,and the examination of antibacterial prescription should be strengthened so as to improve the level of rational use of antibiotics in hospital.

To investigate the effect of the case-centered teaching method in biochemistry teaching. 47 students were divided into two groups :the study group and the control group.The casecentered teaching method was used in the studying group.The control group was taught by traditional method.The final examination showed that the results of the studying group were better than the control group.

0.05).The survival area and capillary density were more favorable in the EPCs-injection sites than the controls(P

To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
Animals ; Apoptosis ; Breast Neoplasms ; drug therapy ; pathology ; Humans ; Inhibitor of Apoptosis Proteins ; genetics ; pharmacology ; Injections, Intralesional ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Transfection

Objective To analyze the expressions of astrocyte elevated gene-1 (AEG-1 )and Beclin-1 (BECN-1 )in papillary thyroid carcinoma (PTC)and their clinical significances.Methods Thirty patients with PTC who were performed total thyroidectomy in the General Hospital Affiliated to Tianjin Medical University from October 201 5 to May 201 6 were collected.All patients were diagnosed with unilateral PTC.The tumor tissues were taken from the patients′ipsilateral thyroids,and 30 cases of normal tissues were taken from the patients′contralateral normal thyroids.The expressions of AEG-1 and BECN-1 in PTC tissues were detected by reverse transcription-poly-merase chain reaction (RT-PCR).The relationships between the expression status and clinicopathologic features were analyzed.Results The positive expression levels [M(QR )]of AEG-1 in PTC and the normal tissues were 0.626 1 (0.741 4)and 0.049 8 (0.01 1 8)respectively,with a significant difference (Z=-4.488,P=0.000). The positive expression levels of BECN-1 in PTC and the normal tissues were 0.067 9 (0.1 98 1 )and 0.785 7 (0.361 7)respectively,with a significant difference (Z=-5.441 ,P=0.000).In PTC,the expressions of AEG-1 and BECN-1 were related to TNMstaging (Z=-3.980,P=0.000;Z=-2.265,P=0.023)and lymphatic metas-tasis (Z=-3.1 90,P=0.001;Z=-2.640,P=0.008),but they were not related to age (Z=-1 .203,P=0.229;Z=-1 .1 62,P=0.245),gender (Z=-1 .222,P=0.222;Z=-0.453,P=0.651 )and tumor size (Z=-1 .496,P=0.1 35;Z=-1 .1 66,P=0.244).The expression of AEG-1 was negatively correlated with that of BECN-1 (r=-0.343,P=0.007).Conclusion The expression of AEG-1 in PTC is higher than that in normal tissues and the expression of BECN-1 in PTC is lower than that in normal tissues,and the expressions of AEG-1 and BECN-1 are related to TNM staging and lymphatic metastasis,which are expected to become prognostic indicators.

Objective To construct and appraise a series of shRNA eukaryotic expression plasmid which contains vary spots of human SKP2 gene.Methods Three pairs of hairpin-like oligonucleotide sequences specific for human SKP2 gene were designed and synthesized. They were cloned into the eukaryotic expression pSIREN-RetroQ plasmid. The recombinated plasmids were confirmed by PCR and sequencing.Results The shRNA sequences were constructed successfully, and could be inserted into the eukaryotic expression vector pSIREN-RetroQ.Conclusions Three shRNA transcripting plasmids pSIREN-RetroQ are successfully constructed, and form a basis for research in cancer gene target therapy against SKP2 gene.