The ability of memory will wear away as human beings become old.These changes are different due to different memory processes and different memory tasks.Researches of the memory changes will help us master the rules of adults' learning and improve the quality of the adult education.

Objective To study the relationship between hepatocarcinogenesis and the mutation in X gene among patients with chronic hepa-titis B virus (HBV)infection,such as chronic hepatitis B (CHB),liver cirrhosis (LC)and primary liver cancer (PLC).Methods The serum samples from 89 patients with chronic HBV infection who visited the Second Affiliated Hospital of Chongqing Medical University from 201 1 to 2013 were collected.PCR was used to amplify the X gene of HBV DNA extracted from the serum samples.After sequencing,the HBV-X genome was compared with those reported in GenBank to find the variable sites and variant forms.Chi -square and one -way ANOVA were used for the statistical analysis afterwards,whereas genotypes were determined by the genotyping tool of the National Center for Biotechnology Information.Results All patients were genotype B or C.Among HBeAg-positive patients,46.2% were genotype B,and 53.8% were genotype C;among HBeAg-negative patients,81.2%were genotype B,and 18.8%were genotype C (P=0.001).PLC pa-tients had a significantly higher risk of mutation in the basic core promoter (BCP)region than the CHB and LC groups (69.2%vs 34.4%and 61.3%,P<0.05);in addition,an evident T-base deficiency was observed at nt1821 site (88.5% vs 53.1% and 71%,P=0.014).Among CHB and LC patients,those with genotype C had a significantly higher risk of BCP double mutation than those with geno-type B (61.5%vs 15.8%,P=0.007;83.3%vs 47.4%,P=0.045).The incidence of BCP double mutation was significantly higher in the low-viral load group (≤106 copies/ml)than in the high-viral load group (>106 copies/ml)(81.3% vs 47.9%,P=0.015). Conclusion The BCP double mutation and T-base deficiency at nt1821 site may play important roles in the development of PLC.

ObjectiveTo observe the effect of ultrashort wave and acoustic frequency on chronic pelvic inflammation.Methods183 patients diagnosed as chronic pelvic inflammation were randomly divided into two groups. Group A(n=80) was treated with ultrashort wave and group B (n=103) was treated with ultrashort wave and acoustic frequency current.ResultsThe clinical cure rates were 1.25% in group A and 15.33% in group B; the efficiency rates were 91.25% in group A and 97.09 % in group B; there was a significant difference between two groups (P<0.01).ConclusionThe effect of ultrashort wave and acoustic frequency current on chronic pelvic inflammation is better than that of single ultrashort wave.

Basement Membrane ; pathology ; ultrastructure ; Biopsy ; Glomerulonephritis, IGA ; pathology ; Humans ; Kidney ; pathology ; ultrastructure ; Kidney Glomerulus ; pathology ; ultrastructure ; Lupus Nephritis ; pathology ; Microscopy, Electron, Transmission ; Paraffin Embedding ; Specimen Handling ; methods

Objective To investigate the effect of cardiopulmonary bypass cardiac surgery on postoperative cognitive function of patients who wre undergoing selective cardiac surgery.Methods Fifty children patients who were scheduled for cardiac surgery were investigated with C-WISC (Chinese revised Wechsler Intelligence Scales for children) preoperative.The children patients were tested at the 7th day and 1 month postoperative by C-WISC and digital span.It could be defined postoperative cognitive dysfunction (POCD) if the C-WISC score decreased 1 SD (standard deviation).Results The incidence of POCD at the 7th day and in the first month postoperative was 40％,and 20％ in pediatric patients who underwent cardiopulmonary bypass surgery.It shows that ascending aorta clip time and cardiopulmonary bypass (CBP)time had negative correlation with intelligence quotient (IQ) (P ＜0.05),but age and education had not any correlation with intelligence quotient in pediatric patients who underwent cardiopulmonary bypass surgery (IQ) (P ＞ 0.05).Conclusions The occurrence of POCD in cardiac surgery children underwent CPB tend to be high,with long time CPB and aortic clip were more likely to have postoperative dysfunction.It shows a close relationship between CPB and aortic clip with POCD.Age and education had no significant influence on postoperative cognitive dysfunction in CPB cardiac surgery children.

Objective To explore the clinical efficacies of ambulatory surgery for inguinal hernia.Methods The clinical data of 1 892 patients with inguinal hernia who underwent ambulatory surgery at the West China Hospital of Sichuan University from November 2009 to June 2015 were retrospectively analyzed.Preoperative examinations including blood routine test, blood bio-chemistry checking, blood electrolytes, coagulation convention, pre-transfusion test, blood type, chest X-ray, electrocardiogram were applied to patients.Operation time was rescheduled after abnormal indexes were corrected for elective surgery.Preventive use of antibiotics was not needed.Preperitoneal space tension-free repair or Bassini method was adopted after local infiltration anesthesia.Anesthesia method, operation method, patching material, operation time, number of patients transferred to inpatient department and reasons and treatment expense were recorded.Ambulatory surgery center did satisfaction survey on patients at the discharge.Patients were followed up at postoperative month 1, 3, 6, 12 regularly by the doctor responsible for the surgery till July 2015, including occurrence of complications and chronic pain, recurred hernia, number of readmission and reasons.Measurement data with normal distribution were presented as x ± s.Results Among 1 892 patients, 1 889 patients underwent operation under local anesthesia and 3 patients were transferred to general anesthesia.There were 1 874 patients undergoing tension-free repair including 1 592 using the material of ultrapro hernia system (UHS) , 264 of ultrapro plug (UPP), 8 of prolene hernia system (PHS), 6 of Gore hernia patch, 4 of 3-dimensional plug (3DP), and 18 patients underwent Bassini without preoperative death.The operation time of 1 892 patients was (28 ± 12) minutes.Three patients were transferred to inpatient department for postoperative cut incision pain, ecchymoma, abdominal distention after satiation and discharged from hospital after treatment.Among 26 patients with complications after discharge, 12 had superficial incision infection and recovered after dressing change without removal of patches, 5 had edema in scrotum and inguinal region, 3 had ecchymoma, 2 had nausea and vomiting, 2 had urinary retention, 2 had disruption of wound, and they all recovered smoothly after symptomatic treatment.Treatment expense of the 1 892 patients was (6 956 ± 249) yuan per hermia.Satisfaction rate of patients was 99.049％ (1 874/1 892) when discharged.The follow-up rate was 86.152％ (1 630/1 892).The follow-up time was 1-67 months with a median time of 35 months.During the follow-up, 4 patients had chronic pain and recovered after analgesia and physiotherapy, 3 patients had recurrence of inguinal hernia including 1 case of recurrent hernia and 2 cases of huge hernia.Conclusion Ambulatory surgery for inguinal hernia is safe and effective, with the advantages of few complications, high satisfactory rate and low hospital expenses.

OBJECTIVE:To establish a HPLC method for determination of the content of baicalin in Bifukang oral liq?uid.METHODS:The ODS C 18 column was used with methanol-water-glacial acetic acid(45∶55∶1.5)as mobile phase,and de?tective wavelength was274nm,the flow rate was1ml/min.RESULTS:Within the range of0.215?g～4.3?g,baicalin presented a fine linear relationship between amount and peak area(r=0.9995).The average recovery was99.44%(RSD=0.79%,n=5). CONCLUSION:This HPLC method is convenient,rapid and accurate,and can be used for quality control in the production of Bifukang oral liquid.

ObjectiveTo observe effects of Fentanyl Transdermal System and Sanlitong on pain of hemorrhoids patients after operation of micro wave heating among tissue.Methods60 hemorrhoids patient whom were treated by micro wave heating among tissue were randomly divided into group A and group B. Patients of group A had Fentanyl Transdermal System affixed on navel position. Patients of group B taken Sanlitong. Effects of two groups were estimated with McGill Questionnaire.ResultsThere was a significant difference between pain scores of two groups in one or two weeks after hemorrhoids operation. The ease pain effect of Fentanyl Transdermal System was better than that of Sanlitong tablet within two weeks after operation.ConclusionThe Fentanyl Transdermal System is a safe, simple and better effect medicament type for ease the pain when treating hemorrhoids with method of micro wave heating among tissue.