1.Studies on Operative Treatment of Huge Rhe gmatogenous Retinal Detachment
Journal of Third Military Medical University 1983;0(04):-
Twelve patients with huge rhegmatogenous retinal detachment were treated with electrocoagulation and/or cryosurgery supplemented with extrascleral buckling, annular knotting or macular sling technique. 11 cases were cured but failure occurred to one case. After treatment, the vision was improved in various degrees in different patients.It is concluded that even though the rhegma is large and the retina is wholly detached, the cure rate can be increased if careful preoperative examination and preparation is made, appropriate operative measures selected, electrocoagulation and cryosurgery reasonably adjusted, and the rhegma securely buckled.
6.Blue light-induced replicative senescence of rat retinal pigment epithelial cells
Yifeng KE ; Song CHEN ; Rui HAO
Chinese Journal of Ocular Fundus Diseases 2009;25(6):462-465
Objective To investigate the relationship between exposure intensity and illumination time of blue light and replicative senescence of rat retinal pigment epithelial (RPE) ceils.Methods Thirty-six 12-14 weeks Wistar rats were kept in the cage with a blue-light bulb [(450±10) nm],and were randomly divided into four groups (no light,nature light,500 lx light and 1000 lx light illumination),each has nine rats.The rats in each group were further divided into three subgroups according to illumination time (one month,two months or three months).Eyeballs were collected after intraperitoneal injection of 10% chloral hydrate.The right eye of each rat was embedded in paraffin and sectioned for hematoxylin-eosin (HE)staining,while frozen sections of the left eye were stained for the senescence-associated β-galactosidase (SA-β-Gal).The data were analyzed by SPSS11.5 statistical software.Results The amounts of SA-β-Gal positive RPE cells were significantly different between all groups under the same illumination time 17 (P=0.000),and between all subgroups of different illumination time with same exposure intensity (P<0.01)except for the control group (no light).Conclusion Blue-light can induce replicative senescence in rat RPE cells in an intensity and time-dependent manner.
7.A study of CD44v6 expression in parotid pleomorphic adenomas
Jukun SONG ; Guanglei HUANG ; Ke ZHAO
Journal of Practical Stomatology 2000;0(05):-
To explore the relationship of the expression of CD44v6 with tumor recurrence in parotid pleomorphic adenoma and its clinical significances,SP immunohistochemical staining was employed in the 10 cases of CPA and 10 cases of PA,10 cases of normal parotids.The relationship of the expression of CD44v6 with tumor recurrence in parotid pleomorphic adenoma was studied.The positive rate of expression of CD44v6 in CPA was much lower than that in PA tissues,the positive rate of expression of CD44v6 in CPA and PA was significantly higher than that in normal parotid tissues.The expression of CD44v6 in CPA tissues was lower than that in PA tissues and may be correlated with tumor recurrence.
9.Sorting methods of breast cancer stem cells
Chongyong GUO ; Keying SONG ; Ke LI
Journal of International Oncology 2011;38(5):358-361
In recent years, the theory of cancer stem cells has provided a new perspective on the treatment of cancers including breast cancer. The accurate sorting of breast cancer stem cells is critical. The sorting procedure is consist of 4 steps: isolation of side population, serum-free suspension culture,determination of specific cell surface markers and of the activity of aldehyde dehydrogenase 1 ( ALDH1 ) enzymatic through the ALDEFLUOR assay. Some studies choose drug resistance as an additional method for sorting cancer stem cells because of the enrichment of cancer stem cells after chemotherapy . The controversy about the sorting outcome of breast cancer stem cell mainly focus on molecular markers like CD44+ CD24- and ALDH1+ . The problem needed to be settled is to identify which sorting method and markers are appropriate.
10.Studies on the immunotherapy of EAE by adoptive transfer of immunoregulatory T cells specific for the spreading determinant
Ling YIN ; Chunjie SONG ; Ke ZHU
Chinese Journal of Immunology 1985;0(03):-
Objective:To explore the therapeutic potential of preemptive targeting of the epitope spreading cascade by adoptive transfer after EAE onset of immunoregulatory T cells specific for the MBP87-99 spreading determinant.Methods:EAE was induced by PLP139-151 in SWXJ mice, all mice were weighed and examined daily for neurologic signs. On days 6 after EAE onset, mice were injected with IL-2prom→IL-10cDNA-transfected Th2/Tr1-like T cells specific for MBP87-99,PLP104-117,or BSA. Finally demyelination was quantified by analysis of digitized images of PLP immunostained spinal cord sections.Results:Mice received Th2/Tr1-like T cells specific for MBP87-99 had a significantly improved clinical outcome(P=0.02) compared with control mice, reduction in the relapse rate and delay in mean time to onset of first relapse were also observed(P