Child ; Critical Illness ; classification ; Emergency Medical Services ; standards ; Humans ; Intensive Care Units, Pediatric ; standards ; Quality Assurance, Health Care ; Severity of Illness Index

Objective To study the level of serum vascular endothelial growth factor(sVEGF)in advanced non-small cell lung cancer(NSCLC) and its clinical significance.Methods Serum level of VEGF was detected by ELISA method in 96 patients with advanced NSCLC.sVEGF level was analyzed after giving 2-4 cycles of cisplatin-based chemotherapy.At the same time,serum level of VEGF in 50 healthy controls was detected.Results The level of sVEGF was significantly higher in patient with lung cancer than in control group (P＜0.01).No statistical significant defference was observed between sVEGF level of stage Ⅲ and Ⅳ NSCLC patients,the patients with squamocellular lung cancer and adenocarcinoma(P＞0.05);Descended sVEGF level was found in the patients with response to chemotherapy(P＜0.05).Conclusion sVEGF can be used to evaluate chemotherapy response and diagnoses in the patients with advanced NSCLC.

Objective:To comparative Research with MSCT and CAG in the myocardial bridge-wall diagnostic coronary. Methods: Selected 93 patients for the study in January 2011 to the end of December 2013,MB-MCA patients diagnosed in Beijing North Hospital, all patients underwent catheter coronary angiography (CAG) and 64-slice spiral CT (MSCT), comparison of the diagnostic accuracy of the two inspection methods, and the results of the correlation analysis, results processing and analysis using statistical software SPSS17.0. Results:MSCT and CAG were two examination methods in the diagnosis of superficial and deep type MB-MCA, with a high degree of consistency in the diagnosis of MB-MCA average length, average depth comparison with statistical significance(t=3.021, t=3.758;P<0.05), there was not statistically significant comparison in the narrow detection rate. Conclusion:MSCT as a novel, non-invasive means of MB-MCA examination, the diagnosis results are reliable, accurate and capable of providing a richer diagnostic information for clinicians, clinician and patient can be widely applied.

Objective: To discuss the value in the differential diagnosis of benign and malignant in ulmonary ground-glass opacities with multislice spiral CT. Methods: Selected 31 patients in Beijing North hospital with spiral CT examination of the chest as the research object, the lungs differential diagnosis of ground-glass image analysis to pathological findings as diagnostic criteria, analysis Imaging features of benign and malignant grinding glass shadow. Results: Malignant lesions with pleural indentation, spiculation, lobulation, such as performance-based, clear boundary between the two groups in the pleural indentation, spiculation, points Ye Zheng, clear boundary comparing with statistical significance(x2=11.138, x2=6.482, x2=4.306, P<0.05). Meanwhile benign lesions showed a round or oval, and malignant lesions with irregular shape and multi-nodular-like performance-based fusion of the two groups in a round or oval, irregular and more fusion-like nodules difference was significant, the statistical significance (x2=11.138, x2=6.482, x2=4.306, P<0.05). Conclusion:Application of multislice spiral CT examination of the chest can be on the ground glass by special signs and morphological comprehensive analysis, benign and malignant, with a high value.

Aspheric intraocular lenses(IOLs)have been shown to provide better quality of vision than spherical IOLs.However,these lenses are not ideal for all patients.Surgeons may have different strategies for compensating for the patients's cornea spherical aberration,targeting a postoperative spherical aberration of zero or +0.10 ?m.But we must know that the negative spherical aberration aspheric IOLs require very good centration with respect to the visual axis of the eye.

Objective To study the relationship between tissue factor expression and cell metastatic ability in two breast carcinoma cell lines. Methods RT-PCR and flow cytometry(FCM) were used to detect the mRNA and protein expressions of tissue factor(TF) in the breast carcinoma cell lines MCF-7 and MDA-MB231, respectively. The metastatic ability of the two breast carcinoma cell lines was determined using modified Boyden chambers. The data were analyzed by t test. Results The mRNA and protein expression levels of TF in MDA-MB231 were obviously higher than those in MCF-7, and the metastatic ability of MDA-MB231 was stronger than that of MCF-7,which was significantly different between the two cell lines. [WTHZ]Conclusion TF expression was positively related with breast carcinoma metastatic ability, and TF may be a therapeutic target for breast carcinoma.

Objective To explore the mechanism of the acute cerebral infarction leading to systemic inflammatory response syndrome (SIRS) which causes multiple organ dysfunction syndrome (MODS), and to explore the role of tumor necrosis factor (TNF-?) and IL-1? in the mechanism from SIRS to MODS.Methods 68 cases of acute cerebral infarction (ACI) were divided into three groups. They were 36 cases of simple acute cerebral infarction (SACI group), 32 cases accompanied with SIRS (SIRS group) and 24 cases accompanied with MODS (MODS group). Enzyme linked immunosorbent assay (ELISA) method was used to measure serum TNF-? and IL-1? contents at different time after ACI and compared with 28 healthy persons.Results (1) 47.06% of the 68 cases were accompanied with SIRS, of whom 75.00% were accompanied with MODS.(2) The serum TNF-? and IL-1? levels were the highest in MODS group, next in SIRS group, then in SACI group, the lowest in control group. The differences were significant among 4 groups (all P

Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application.

Objective To analyze cardiac function of patients with first onset acute myocardial infarction (AMI)by speckle tracking imaging (STI).With clinical data of long-term follow-up,to explored the prognostic value of left ventricular functional reservation and damage after AMI.Methods Seventy-eight patients with first onset AMI were enrolled in the study.Dynamic images were acquired before primary percutaneous coronary intervention for the analyses of longitudinal peak systolic strain (LPSS), radial peak systolic strain (RPSS)and circumferential peak systolic strain (CPSS).Clinical data of long-term follow-up,results of electrocardiogram and coronary angiography were used for the evaluation of the recurrence risk of cardiac events and cardiac death after AMI.All the baseline clinical data and parameters of echocardiography were compared between patients with and without cardiac events and cardiac death during follow-up.Results Baseline clinical data of electrocardiogram and coronary angiography didn't show significant differences for the comparison of AMI patients with and without cardiac events and cardiac death during long-term follow-up.Compared to patients without cardiac events during follow-up,LPSS and CPSS (P <0.001)of patients with cardiac events were relatively lower and the prognostic value of CPSS (HR=1 .4096)for the recurrence risk of cardiac events was better.For the risk prediction of cardiac death,LPSS ( P <0.001,HR=1.5735)was relatively better.Conclusions Left ventricular functional reservation and damage after AMI were accurately assessed by STI,which helped predicted the recurrence risk of cardiac events and cardiac death of AMI patients.CPSS and LPSS were optimal indicators for the prediction of cardiac events and cardiac death,respectively.