Objective:To study the effects of curcumin on the activity of ATPase and the mechanism of apoptosis in U-251 cell.Methods:U-251 cells were treated with 20,40,80,100?mol/L curcumin for 24 h and the growth inhibition rates of U-251 cells were measured with MTT method.Cell apoptosis was inspected with flow cytometry(FCM).The activities of ATPase were determined by colorimetry method.Results:Curcumin inhibited the proliferation of U-251 cells and induced apoptosis of U-251 cells.level of ATPase in U-251 plasma Membrane was low remarkably.Conclusion:Curcumin induced apoptosis and inhibited proliferation of U-251 cell via inhibition of activation of plasma Membrane ATPase.

Five male and one female patients with IPVS caused by infarction of occipital cortex and optic radiations of brain were reported. The ages were in the range from 21 to 69 years. The evidence of diagnosis included sudden loss of visual acuity in both eyes, normal fundus, homonymous defect of visual field, infarction of brain in CT scan, without other positive signs of central nervous system or headache or eye pain before or at the time of attack. All the patients were treated by injection of Co- Anisodine Ⅰ and Ⅱ at acupuncture points with satisfactory effects. The therapy is safe, effective, and reliable. The clinical features and the treatment mechanism were disscused.

Thirty-six patients with normal vision in one eye coupled with advanced age-related macular degeneration(AMD)in the fellow eye,and 36 eyes with drusen in one eye coupled with macular drusen in the fellow eye,and 36 normal subjects(36 eyes) were measured by FM100-Hue test.All the tesed eyes had corrected vision of 1.0 or better and had drusen in the fundus.The results showed that the total errors score of FM100-Hue test of patients whose fellow eye suffered from advanced AMD compared with that of the patients whose fellow eye did not suffer from advanced AMD,difference between the two groups in the red and panchromatic regions was statistically significant(P

Visual function in early age-related macular degeneration (AMD) were observed by contrast sensi-tivity(cs), macular threshold, FM 100-Hue test, dark adaptation, and the Amsler grid. All patients with early AMD achieved corrected visual acuity of 1.0 or better. The results revealed that early AMD showed one or more of following: abnormal macular light sensitivity (MLS), hue discripmination, CS, threshold of dark adaptation, and Amsler grid, comparing with those of the normal controls. These tests proved useful for the early diagnosis of AMD, and their sensitivity was found to be in the following descending order: MLS, FM100-hue, CS, and Amsler grid.

Animals ; Biopsy, Needle ; Humans ; Kidney ; ultrastructure ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron, Transmission ; methods ; Specimen Handling

Objective The experiment compared the chemical components in the roots,stems,and leaves of Dracaena cochinchinensis with those in the raw material of Dragon's blood,in order to find the possible raw material of Dragon's blood.Methods Technology of thin layer chromatography(TLC) and high performance liquid chromatography(HPLC).Results The results showed that the roots,stems,and leaves of D.cochinchinensis just contained small amount of loureirin A,the contents of loureirin A of the three samples are: 58,53,and 15 ?g/g,respectively.Loureirin B was only detected in the leaves with content of 2 ?g/g.Conclusion From the analysis of peak areas and the retention time,there are only slight correlations among the components of the four samples and the chemical components among the roots,stem,and leaves are different.Moreover,there is too low content of loureirins in the roots,stem,and leaves to be raw material of Dragon's blood.

Objective To investigate the association between M235T polymorphism of angiotensinogen (AGT) gene and cerebral infarction(CI) in Chinese.Methods AGT gene M235T polymorphism was examined in 75 cases with CI and 48 healthy controls by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results The frequencies of AGT gene T235 allele and 235TT genetype were 0.78 and 0.64 respectively,compared with those of the control group (0.604 and 0.375 respectively ),the difference was significant(? 2=8.82, P

The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a focused research topic in recent years.This article reviews the up-to-date research advances in the features and mechanisms of immune regulation of MSC and its application.

Microvesicles (MVs) is small membrane vesicles released from different cell types under different conditions.Studies have shown that MVs may mediate vascular inflammation,angiogenesis,and other pathological processes.MVs may play an important role in the pathogenesis of diabetic retinopathy (DR) by mediating endothelial cell injury,thrombosis and neovascularization.The plasma MV level may be an effective parameter to monitor the development of DR.This article will summarize the research progress of the relationship between MVs and DR in recent years.

Character Repertoires are supported by DICOM to encode Characters in different languages, in which UTF-8 and GB18030 are for Chinese characters. Each of them can replace Acquiesced Character Repertoire in Attribute Specific Character Set so that Chinese characters can be encoded in data elements. Utility programs compatible with DICOM are also required to support UTF-8 or GB 18030 for unerring representation and encoding.