Objective To explore the effect iveness and safety of ProSeal laryngeal mask(PLMA)used in posterior spinal surgery. Methods Forty ASA Ⅰ - Ⅱ patients of thoracic-lumbar fracture were randomly divided into PLMA group and tracheal intubation (TI) group by systematic sampling with 20 cases each. PLMA or TI was inserted after intravenous anesthesia induction. The number of intubation, intubation time and time to surgery were recorded, systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were recorded induction (To), during intubation (T1), at 1 min (T2),3 min(T3), 5 min (T4), 10 min after intubation (T5), during extubation (T6). The intubation, extubation, and the maintain ventilation process of the respiratory and respiratory damage were recorded. Results All patients in PLMA group and TI group achieved satisfactory lung ventilation at the first attempt. There were no significant difference in the number of intubation, intubation time and time to surgery between two groups (P ＞ 0.05 ).SBP,DBP,HR at T1,T2,T3,T6 in TI group were significandy higher than To and those in PLMA group (P＜0.05). The intubation, extubation, and the maintain ventilation process of the respiratory and respiratory damage in TI group (5,25,36 cases) were more than those in PLMA group (0,1,6 cases)(P ＜0.05).Conclusion PLMA for posterior spinal surgery is safe and effective.

Objective To compare the effect of proseal laryngeal mask airway(PLMA)and esophageal tracheal combitube(ETC)used in adult patients of difficult airway in perioperation.Methods Forty ASA Ⅰ-Ⅱadult patients of difficult airway were randomly divided into two groups:PLMA group and ETC group,each group was 20 cases.After intravenous anesthesia induction,PLMA or ETC was inserted by routine methods.The number of intubation,intubation time and time to surgery were recorded.The systolic blood pressure(SBP),diastolic blood pressure(DBP)and heart rate(HR)were recorded before intubation (T_1),during after intubation(T_2),at 1 min(T_3),5 min(T_4),10 min(T_5)after intubation,during extubation(T_6).The intubation,the extubation and the maintain ventilation process of the respiratory complications and respiratory damage were recorded.Results All patients in PLMA group and ETC group achieved satisfactory lung ventilation at the first attempt.The number of intubation,intubation time and time to surgery were not different between the two groups(P＞0.05).SBP,DBP,HR at T_2,T_3,T_6 in ETC group [T_2:(143.2±11.4)mm Hg(1mm Hg=0.133kPa),(86.9±10.7)mm Hg,(145.8±10.2)times/min,T_3:(140.0±11.3)mm Hg,(84.5±10.7)mm Hg,(142.5±11.5)times/min,T_6:(147.7±11.3)mm Hg,(87.2±9.7)mm Hg,(145.4±10.9)times/min]were significantly higher than those in PLMA group[T_2:(123.5±12.7)mm Hg,(72.6±11.4)mm Hg,(129.0±11.0)times/min,T_3:(120.0±11.5)mm Hg,(69.7±10.5)mm Hg,(125.7±10.6)times/min,T_6:(122.5±11.6)mm Hg,(71.4±9.4)mm Hg,(127.8±11.3)times/min]and at T_1 in ETC group[(122.7±12.1)mm Hg,(74.1±9.6)mm Hg,(121.0±11.8)times/min](P<0.05).Respiratory complications and damage were no significant difference between the two groups(P＞0.05).Conclusion PLMA and ETC can achieve satisfactory lung ventilation used in adult patients of difficult airway in perioperation,they are safe and effective airway management device,but PLMA used in adult patients of difficult airway in perioperation is better than ETC in keeping stable hemodynamics and producing less stress responses.

To review the literatures on the treatment of sleep disorder with acupuncture and moxibustion in China in recent years. we believed that combined acupuncture and medicine, acupuncture and auricular acupuncture had obvious advantages in the treatment of sleep disorder after stroke.

Objective To measure the magnitude of galvanic currents produced by different occluding metallic contact.Methods The circuits of instantly different metallic contact were simulated in artificial saliva,and electrical potential of every couple and 15 currents of instant contact were measured.To simulate night sleep,after 8 h soaking,15 currents were obtained in addition.Results During early soaking,gold/Co-Cr alloy produced the highest current(6.17 ?A),while there were significant differences in currents between gold/zinc-free amalgam,gold /zinc-containing amalgam and other couples(P0.05),and the current value was low all the time.Potential of zinc-containing amalgam was more negative than other alloys,and it was always under anodic behavior during the experiment and was corrupted acceleratedly.Conclusion Couples of gold/titatium,gold/zinc-containing amalgam,zinc-free/zinc-containing amalgam appearing in oral will do harm to the patients′health,while Co-Cr/zinc-free amalgam can coexist in oral.Corrosion behavior of amalgam is affected by different contents of zinc.

Abstract Benzo [a] pyrene ( B [a] P ) is a well-recognized environmental pollutant. Exposure to B[a]P elicits many adverse biological effects, including tumorigenesis, immunosuppression, teratogenicity, and hormonal effects. In addition to B [a] P exposure-induced genetic damages, a growing number of studies demonstrate that epigenetic changes play an important role in chemically induced carcinogenesis. In order to provide better understanding of epigenetic changes of B [a] P and their potential association with genotoxic endpoints, this review summarizes the advances in the applications of functional genomics in the research of B [a] P toxicity, including functional genomics techniques, regulation of human genome expression, DNA sequence variability, model organisms research, and bioinformatics studies, so as to provide insights into the management of B [a] P exposure-induced health injuries and use of genomics techniques to unravel the mechanisms underlying the toxicity of other environmental pollutants.

OBJECTIVE To investigate the distribution and antibacterial resistance of nosocomial infection from a new hospital pathogens,and provide reference for clinical rational use of drugs.METHODS Statistical methods were used to analyze the data of pathogen′s formation,distribution and antibacterial resistance of a new hospital.RESULTS A total of 1852 clinical isolates were collected from Mar 2005 through Mar 2007,of which Gram-negative microorganisms and Gram-positive cocci accounted for 71.0 % and 29.0%,respectively.The most commonly encountered pathogens were Pseudomonas aeruginosa,Escherichia coli,Klebsiella spp,Acinetobacter,Staphylococcus aureus and coagulase-negative Staphylococcus.Except Acinetobacter,up to 6% strains of Gram-negative microorganisms were resistant to the imipenem,meropenem and piperacillin/tazobactam;Gram-positive cocci were still highly sensitive to vancomycin and teicoplanin.CONCLUSIONS A new hospital is similar with other hospitals in the data of formation,distribution and antibacterial resistance of nosocomial infection pathogens.

Hypoxia-inducibIe factor-1(HIF-1)is a main nucIear transcription factor for response to intratumor hypoxia. RecentIy,it has become a hot spot to attempt to improve hypoxia by inhibiting HIF-1. HIF-1 inhibitors' mechanisms of action are wide,which infIuences the two signaI transduction pathways that are phosphatidyIinositoI-3-kinase/ protein kinase B/ mammaIian target of rapamycin and Ras/ Raf/ mitogen-activated protein kinase kinase1-2/ extraceIIuIar signaI-reguIated kinase1-2,inhibits the function of heat shock protein 90,and reduces transcriptionaI activity of HIF-1. In combination with radiation,HIF-1 inhibi-tors significantIy enhance radiosensitivity and weaken radioresistance of hypoxia ceIIs,which contributes to the therapeutic effect of tumor irradiation.

Objective To observe inhibitory effect of recombinant human endostatin on the proliferation of vascular tumor endothelial cells and to investigate its possible mechanism.Methods Hemangioma endothelial cells were cultured in vitro and different concentrations of endostatin on hemangioma endothelial cell proliferation inhibition were detected by MTT method.Effect of recombinant human endostatin on endothelial cell cycle was detected by flow cytometry.The expression of VEGF, KDR mRNA in hemangioma endothelial cell were detected by Real-time RT PCR. Results Recombinant human endostatin concentrations in 24 h, 48 h and 72 h after three period during which the hemangioma endothelial cells inhibited significantly( P <0.01 ) , and there was a clear dose dependence, IC50 was 355 μg/mL.Recombinant human vascular endostatin ( 250 μg/mL ) intervented for 24 hours, the proportion of cells in G0/G1 phase(94.23 ±1.66)%, compared with control group (90.63 ±1.14)%, had significantly difference (P<0.05).Compared with the control group, the difference of the expression of vascular endothelial growth factor (VEGF) was statistically significant (P<0.05) as well as Flk-1 (P <0.05).Conclusion Recombinant human endostatin does not only have inhibitory effect of hemangioma endothelial cell cycle, but also can inhibit the expression of VEGF and FLK-1.

Objective To investigate the effects of single and combined exposure to ozone and nitrogen dioxide on airway injury and inflammatory levels in mice. Methods Eighty BALB/C mice were randomly divided into eight groups:four were sensitized model groups(the control group,the O3 group,the NO2 group and the O3+NO2 group based on the way of exposure) ,the other four were non-sensitized groups. The concentration of O3 was 0.16 mg/m3,NO2 was 0.30 mg/m3. Mice were exposed 2 hours every day for 7 consecutive days. Mice were sacrificed in the exposure end and cell counting and cytokine measurement from bronchoalveolar lavage fluid(BALF) were performed. Results In the sensitized groups,neutrophil proportion and IL-6 were significantly increased except the control group and IL-2 and MDA were also significantly increased in the O3 group and the O3+NO2 group(P

0.05).[Conclusion]Allogeneic freeze-dried bone marrow stromal cells has better biocompatibility but no cytotoxicity.It provids experimental data for its clinical application.