OBJECTIVE: To prepare fumeisong cream and establish its quality control method. METHODS: Ingredients including carbamide, triamcinolone acetonide acetate, camphor, and laurocapram were mixed in an oil-in-water matrix for preparation of fumeisong cream. HPLC was adopted to determine the content of triamcinolone acetonide in the cream. RESULTS: The prepared cream was white in color, with test items all in line with specifications stipulated in 2005 version of China Pharmacopoeia. The linearity concentration range of triamcinolone acetonide was 2.06～20.6 ?g?mL-1(r=0.999 6) and the average recovery rate was 99.6% (RSD=1.0%,n=9). CONCLUSION: The cream is simple and feasible in preparation techniques, and its quality is stable and controllable.

OBJECTIVE:To investigate the therapeutic efficacy of celecoxib in the treatment of knee osteoarthritis and its ef-fects on patin degree. METHODS:One hundred and twenty cases of knee osteoarthritis were selected from our hospital during Jan. 2014 to Jan. 2016,and then divided into control group and observation group according to therapy plan,with 60 cases in each group. Control group was given Ibuprofen sustained-release capsule 0.3 g,bid;observation group was additionally given lornoxi-cam 8 mg,bid,on the basis of control group. Both groups were treated for 4 weeks. VAS score was compared between 2 groups before and after treatment,and clinical efficacy and the occurrence of ADR were observed in 2 groups. RESULTS:There was no statistical significance in VAS score between 2 groups before treatment(P>0.05). After treatment,VAS score of 2 groups were de-creased significantly,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). Clinical response rate of observation group was 95.0%,which was significantly higher than 75.0% of control group,with statistical significance (P<0.05). The incidence of ADR was 3.33% in observation group,which was significantly lower than 11.67% of control group,with statistical significance(P<0.05). CONCLUSIONS:Lornoxicam is effective for knee osteoarthritis and can significantly improve pain with good safety.

With the further study on heterogeneity and molecular mechanism of diffuse large B-cell lymphoma (DLBCL),many novel targeted drugs have been developed,which also showed clinical benefit in early-stage studies.Therapeutic targets for DLBCL mainly include the surface antigens of B lymphocyte,B cell receptor signaling and the cellular microenvironment.Several new clinical trials about targeted therapy for DLBCL had been reported in the 55th ASH annual meeting,and the results were encouraging.The advances in this field will be summarized in this paper based on the new reports in the 55th ASH annual meeting.

ObjectiveTo investigate the diagnostic and prognostic significance of serum human kallikrein11 ( KLK11 ) in patients with ovarian cancer. MethodsThe level of serum KLK11 was determined by enzyme-linked immunoassay(ELISA) for 30 patients with ovarian cancer, 26 patients with benign gynecologic processes and 32 healthy subjects,and the KLK11 value beyond the upper-limit of 95％ credibility interval( 95％ CI) based on the health controls was used as the positive value. The positive rate of KLK11 was compared among the three groups.ResultsThe positive rate of KLK11 in patients with ovarian cancer was significantly higher than those in patients with benign gynecologic processes or in healthy subjects[(73.3％) and(7.7％ ) or(6.3％ ) ,all P ＜0.01 )] ,and there was no significant difference between ovarian benign tumor group and the healthy control group ( P ＞ 0.05 ).ConclusionThe serum KLK11 should be a novel tumor marker in the clinical diagnosis of ovarian cancer.

A model system which simulated the conditions of the human stomach was used in the experiments. Precursors NaNO2 and MNG formed MNNG and resulted in the positive mutagenic response to Salmonella typhimurium TA 100. When the concentration of the precursors was 50 mM NaNO2 and 100 mM MNG, the induced average number of revertant per plate was 4327 showed an approximate twenty fold spontaneous revertants. With diluting of the precursors, the mutagenic response was lower, showing a dose-response relationship between the mutagenic activity and the precursors concentration. It was found that, when precursor concentration was lower than 22.2 mM NaNO2 and 44.4 mM MNG, the formation of MNNG was blocked completely by the Chinese Kiwi fruit juice and mutation was inhibited. In the same concentration without the Chinese Kiwi fruit juice, the induced revertant was an approximate 13 fold of the spontaneous revertant. The Chinese Kiwi fruit could not block the formation of the MNNG when the precursors concentration was higher than 33.3 mM NaNO2, but it inhibited the mutagenic activity partly. Compared with the Chinese Kiwi fruit juice, the effect of the ascorbic acid solution in the same concentration was much less.It was demonstrated with TLC that MNNG was formed in the model system.

In order to observe the effect of Actinidia chinensis Planch fruit juice on blocking the formation of N-nitrosamide in vivo, 99 pregnant Wistar rats were divided into five groups. The rats of each group were orally given ni-trosamide precursor ethylurea (0.25, 0.50, 1.00, 2.00 4.00 mmol/kg) and NaNO2 (0.125, 0.25, 0.50, 1.00, 2.00 mmol/kg) respectively once a day for three days, on the 7th, 8th and 9th day of pregnancy. Half of the rats in. each group were administrated two precursors and a 4% starch solution, while the other half were administrated two precursors and the tested fruit juice.In the groups without the juice, NEU was formed in vivo, which led to a high mortality of embryos. The embryolethality was 5.21%, 43.66%, 71.7%, 85.8% and 100% respectively, and 4 pregnant rats died in the highest dosage group. However, the rats who received both the two precursors and the tested fruit juice, the living embryos and embryolethality were similar to the control groups except the highest dosage group. The none treatment group and groups only given one of the precursors were done as control groups. The results suggested that the concentrated juice could block the formation of NEU in vivo and prevent the embryotoxicity of NEU.

Hair can be easily collected,the collection method does not cause any harm to body.It can provide long and convinced information about drug uses.It possess irreplaceable advantages of blood,urine and other biological samples.The review summarizes the basic information and recent studies of hair analysis.It introducs the hair-shaft structure,the mechanisms of drug incorporation,extraction/purification methods and all kinds of analytical techniques.The advantages,drawbacks and promising prospects about hair in drug monitoring are also discussed.

Objective:To study cardiomyocyte apoptosis of viral myocarditis (VMC) mice,study the effect and significance of Tumor Necrosis Factor-? (TNF-?) to cardiomyocyte apoptosis in VMC.Methods:Our experiments studied cardiomyocyte apoptosis at 3、5、7、9、15、35 days after virus inoculation in VMC mice by ways of TUNEL and electron microscope.We studied the effect of TNF-? to cardiomyocyte apoptosis in VMC by the way of intervention with TNF-? and TNF-? mAb.Results:Apoptotic cardiomyocyte of VMC mice increased appaently compared with control mice.Cardiomyocyte apoptosis increased after TNF-? intervention,cardiomyocyte apoptosis reduced after TNF-? mAb intervention.Conclusion:Abnormal cardiomyocyte apoptosis was observed in VMC,cardiomyocyte apoptosis may has relation with the Pathogenesis of VMC.TNF-? can induce cardiomyocyte apoptosis in VMC.TNF-? induces cardiomyocyte apoptosis may has relation with its role in VMC.

[Objective] To design anatomical steel plate for internal fixation of scapular neck fracmre.[Method]By observing the scapular structure and scapular fracture type,imaging a form of anatomical steel plate for internal fixation of scapular neck fracture,the needed relative measurement datum of scapula were obtained.Forty pairs of scapular spechnens of men and women were measured,relative datum were obtained.Based on the measurement,scapular neck fracture plate was designed.The steel plates were applied in patients with scapular neck fracture or scapular body fracture,patients were followed up after operations,the results were observed.[Result]Seven patients with scapular neck fracture or scapular body fracture were operated with the anatomical steel plates,the steel plates were obedient to the scapulas greatly,the fixations were strong,the operations were easy,the time of operations were short,seven patients were followed up for 12-18 months showing satisfactory results.[Conclusion]The design of anatomical scapular neck fracture steel plates is satisfactory,when using tile plate to fix scapular neck fracture.The fixation is strong,using the steel plate can be benificial to patient's early exercise.It has advantages of preventing complications,promoting fracture to heal,making the scapular operation easy,shortening the time of operation,lessening the blood in operation.This type of anatomical steel plate has the value to be popularized.

According to traditional theory tumor growth arised from the proliferation of all tumor cells, therefore management mainly aimed at the majority of the cells within cancer tissue. As a result, relapse and metastasis developed often, which caused treatment failure. It was stem cell that makes it possible to treat cancer radically and prevent it from relapsing and metastasizing by specifically killing targeted cancer stem cells and even some old concepts such as tumorigenesis mechanism, cell signaling pathways etc may need to be reevaluated, that raised great challenge to traditional oncotherapy. In this text the origin and existing evidence of cancer stem cell, it's relationship to common stem cell, clinical significance and prospect were reviewed.