Multiple scientific technologies are used in modern 120 emergency command center. This paper introduces the frame of l20 emergency command center and the functions of CTI and 3G technology,and analyzes the practical application and effects of CTI and 3G in modern 120 emergency command center.

Objective To observe the retinal toxicity of repeated intravitreal injection with bevacizumab(Avastin)in diabetic rats.Methods Forty male Sprague Dawley(SD)rats were randomly divided into normal group(Group A,10 rats)and diabetes mellitus group(30 rats).The rats in diabetes mellitus group were induced with streptozotocin injection for diabetic retinopathy model.And then randomly divided into diabetic retinopathy(DR)group(Group B,10 rats),the rats were not intervened;the left eyes of the other 20 rats were intravitreal injected with bevaeizumab 3 μ1(25 mg/m1)for 3 times as experimental group(Group C);the right eyes of the 20 rats were not intervened as experimental control group(Group D),20 days after last intravitreal injection,retinal function was measured by Flicker Electroretinogram (F-ERG);retinal vascular pattern was determined by fluorescence microscopy of ethidium bromide(EB)stained retinal flat mounts;retinal morphological changes were determined by light microscope on hematoxylin-eosin (HE) stained sections;Thy-1 and VEGF expression was measured by immunohistochemistry staining.Results F-ERG showed that-the differences of a-and b-waves-the b-wave amplitude and the Ops-wave amplitude in the implicit time between group A,B,C and D were significant (F=33.165,36.162,19.955,23.243;P=0.000);the differences of a-wave amplitude between group A,B,C and D was not significant(F=0.097,P=0v961).Retinal blood vessel pattern was normalin Group A;retinal vascular vessels were tortuous and irregularly expanded in Group B:retinal vascular vessels of Group C were regular and thinner than Group A;microaneurysm were showed in Group D.Light microscope displayed that the layers of the rat retina of Group A were regular,the retinal architectures of Group B were irregular,the retinal layers were regular in Group C,the retinal layers were irregular in Group D.Immunohistochemistry staining discovered that Thy-1 and VEGF were mainly expressed in ganglion cell layer(GCL).Conclusion Repeated intravitreal injection of bevacizumab iS toxic tO retina of diabetes mellitus rats.

Thrombin activatable fibrinolysis inhibitor (TAFI), a novel regulator of coagulation and fibrinolysis, was discovered recently. The activated TAFI (TAFIa) makes fibrinolysin lose its action sites with fibrin, and thus exerts its fibrinolytic inhibition and promotes thrombosis. Making a thorough study of TAFI may provide a new option for the treatment of ischemic cerebrovascular disease.

Objective To observe the effect of reteplase on the thromboelastogram tracing of patients experiencing acute is-chemic stroke(AIS).Methods Selected 43 AIS inpatients in the Third People’s Hospital of Liaocheng from October,2013 to August,2015,who received reteplase less than 4.5 hours after the onset of syndrome of AIS.Blood samples were obtained before reteplase administration and at 0.5,1,2 and 4 hours after reteplase administration and measured by thromboelastogra-phy.Results The parameters R,K,Angle and MA of the thromboelastogram had changed after beginning administration of reteplase.The R value at 0.5 h after thrombolysis was significantly higher than that before thrombliysis,however,the R Value at 1h after thrombolysis was the most highest,and the K value.At 0.5 h after thrombolysis was the highest.At 0.5 h after thrombolysis MA and Angle values were the lowest,the differences were statistically significant (P<0.001).Conclu-sion The study suggests that the thromboelastogram is an useful tool for determining changes in the coagulation system of patients receiving reteplase.

Autophagy is the cell biology process in which cytoplasmic components are degraded in lysosomes to maintain cellular homeostasis,it is involved in the pathophysiology of several kidney diseases (podocytopathies,IgA nephropathy,diabetic nephropathy,hereditary kidney disease),but its specific effect is still controversial.Most studies suggest that autophagy plays a protective role in kidney diseases,in the future,it may become a new target for the prevention and treatment of kidney disease.

Objective To Study the characteristics of the high site visual field injury and the LED-VEP after the head trauma for the purpose of the medico-legal appraisals. Methods 11 cases with cortical blindness resulted from the head injury were studied and then compared with 20 cases suffered from optical nerve injuries with respect to the clinical manifestations and characteristics of the visual field injury and LED-VEP. Results As compared with the characteristics of the optical nerve injuries, besides the subjective complain of lowering the visual field, there were not any positive findings in pupil reflex of light and retina examination in case of the high site visual field injuries. The electrical visual field test revealed the homony-mous hemianopia while the internal segment optical nerve injury manifested unilateral visual field defect. 8 out of 11 cases with cortical blindness, occipital cortex were injured and the flash VEP demonstrated the slight prolongation of the latent period of the PI wave of both eyes, while the PI amplitudes were normal. 3 out of 11 cases were suffered from the subcortical injuries and were manifested with the prolongation of the latent period of the PI wave and the amplitude of the PI wave were significantly reduced. Conclusion Bilateral homonymous hemianopia were the characteristics of the high site visual field injury while the abnormal rate of the flash VEP of the high site visual field was quite low. So that, homonymous hemianopia may be helpful for the diagnosis of cortical blindness. At the same time CT scanning and the electroen-cephalography (EEG) also must be considered comprehensively in the medico-legal appraisals.

BACKGROUND: Vitapex is an injectable root canal filling material. It possesses effects of antibiosis, analgesia, as well as promotes formation of alveolar bone, dentin and cementum, which had been widely used in apexification. However, it also has weakness of long induction time and need reduplicated subsequent visit.OBJECTIVE: To explore the therapeutic effect of mineral trioxide aggregate (MTA) and Vitapex paste on apexification.DESIGN, TIME AND SETTING: The contrast observation experiment was performed at the Department of Stomatology,Shenyang Children's Hospital from February 2006 to November 2008.PARTICIPANTS: A total of 42 patients with 49 teeth were included in this study, including 28 anterior maxilla and 21 premolars.The X-ray film showed that the root tip of teeth was immature.METHODS: The teeth were randomly divided into the experimental group (n=25) and control group (n=24), which was apexificated by MTA and Vitapex paste, respectively.MAIN OUTCOME MEASURES: The effective rate of two groups was compared at months 3, 6 and 12 after operation.RESULTS: All teeth were included in the final analysis. The effective rate of experimental group was greater than the control group at 3 months after operation, and the difference had significant (P < 0.05), while, the effective rate was similar in two groups at 6 and 12 months after operation (P > 0.05).CONCLUSION: Both MTA and Vitapex paste are ideal filling materials in apexfication. Nevertheless, MTA may be a substitute in apexfication for its short therapy duration and precisely therapeutic effect.

Basement Membrane ; pathology ; ultrastructure ; Biopsy ; Glomerulonephritis, IGA ; pathology ; Humans ; Kidney ; pathology ; ultrastructure ; Kidney Glomerulus ; pathology ; ultrastructure ; Lupus Nephritis ; pathology ; Microscopy, Electron, Transmission ; Paraffin Embedding ; Specimen Handling ; methods

Ki-67 is a common proliferation marker,especially used to reflect the proliferation activity of malignancy cells.Recently this marker has been widely studied among early breast cancer patients,However,to date,whether include Ki67 in the list of required routine biological markers there is still a hot argument.This review mainly explores and summarizes the prognostic and predictive role of this marker in breast cancer,the clinical value of the combination of Ki-67 and other makers,and the problems lie in the application of Ki-67 in clinical practice.

Random clustering was conducted among a total of 1068 residents aged 18 years or older at a certain Shanghai community.And they completed a questionnaire survey.Logistic data processing and model building were performed.The respondents were willing to go to a community health center for the following reasons:close proximity (88.4％),excellent service attitudes (40.4％) and low drug prices (37.8％).No statistically significant differences existed in terms of first choices of community health service,occupation,monthly income and-walking distance (P ＞ 0.05).However,statistically significant differences existed in education level,age and chronic diseases (P ＜ 0.05).