Objective: To observe the effect of acupuncture around the diseased region combined with point injection on post-herpetic neuralgia. Methods: Point injection on 30 cases in the related segments of spinal cord after needling around the diseased region. Results: Twenty-seven cases got effect and the total effect rate was 90%. Conclusion: Acupuncture combined with point injection has remarkable curative effect on post-herpetic neuralgia.

Objective To explore the role of plasma level of homocysteine ( Hey ) in progressive ischemic stroke (PIS)of carotid artery system and to investigate the relationship between Hcy and carotid artery atherosclerosis(AS).Methods Plasma Hcy concentrations were measured in 68 patients with progressive ischemic stroke of carotid artery system (PIS group)and 72 patients with non-progressive ischemic stroke of carotid artery system ( non-PIS group ).Plasma Hcy level and carotid artery intima-media thickness ( IMT ) were compared among PIS group and non-PIS group as well as normal control group.The linear correlation between plasma Hcy level and IMT in PIS group was analyzed.Results Plasma Hcy concentrations was respectively (22.52 ± 5.26) μmol/L,( 16.38 ± 4.23) μmol/L and( 10.13 ± 2.16) μmol/L in PIS group,non-PIS group and normal control group( F =56.87,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant ( P ＜ 0.01 ).And there was also significant difference on plasma Hcy levels between PIS group and non-PIS group( P ＜ 0.01 ).The thickness of carotid artery IMT was respectively(1.12 ± 0.34)mm,(0.96 ± 0.27)mm and(0.43 ± 0.21 )mm in PIS group,non-PIS group and normal control group( F =28.47,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant( P ＜ 0.01 ).And there was also significant difference on IMT between PIS group and non-PIS group ( P ＜ 0.01 ).In PIS group plasma Hcy level was positively correlated with the carotid artery IMT ( r =0.687,P ＜ 0.01 ).Conclusion The plasma Hcy level is closely related to the occurrence and development of carotid artery atherosclerosis and PIS of carotid artery system,detection of plasma Hcy level helps to predict the occurrence of PIS of carotid artery system and provide an evidence for the therapy decision.

Objective To investigate the correlation of smokers in wnt/β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.Methods In the Department of Respiratory Medicine of the People's Hospital of Wuhan University,30 cases lung squamous cell carcinoma patients with a history of smoking,30 cases lung squamous cell carcinoma patients with no history of smoking,30 cases bronchiectasis patients with a history of smoking,30 cases bronchiectasis patients with no history of smoking were chose respectively as A group,B group,C group and D group.After the patients were treated by surgery,the removal of diseased tissue used immunohistochemical staining for CD68 + testing,TNF-α,wnt-2,β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method,then wnt-2,β-catenin,TCF-4 transcription of mRNA concentrations were detected by using RT-PCR method.Results Immunohistochemical studies showed that cells in TNF-α,CD68 + expression of A group,B group,C group and D group were respectively 0.895±0.036 and 0.931±0.237,0.125±0.018 and 0.631±0.064,0.083±0.042 and 0.311±0.062,0.053±0.004 and 0.196±0.020,the differences were statistically significant(P＜0.05).Westblot test Results showed that wnt-2,β-catenin,TCF-4 expression level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05),testing of RT-PCR showed that wnt-2,β-catenin,TCF-4 translation level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05).Conclusion Smoking for body wnt/β-catenin pathway has a cell activation,after cigarette activate wnt/β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt/β-catenin signaling transduction circumstances.The detection of wnt/beta-catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.

Objective To explore the applications of im aging exam ination on rib fracture sites in forensic identification. Methods Features including the sites, num bers of the processed im aging exam ination and the first radiological technology at diagnosis in 56 cases of rib fractures from 163 injuries were retro-spectively analyzed. Results The detection rate of the rib fractures within 14 days was 65.6% . The ini-tial detection rate of anterior rib fracture proceeded by X-ray was 76.2%, then 90.5% detected at a sec-ond tim e X-ray, while the detection rate of CTwas 66.7% and 80.0%, respectively. The initial detec-tion rate of rib fracture in axillary section proceeded by X-ray was 27.6%, then 58.6% detected at a second tim e X-ray, while the detection rate of CTwas 54.3% and 80.4%, respectively. The initial de-tection rate of posterior rib fracture proceeded by X-ray was 63.6%, then 81.8% detected at a second tim e X-ray, while the detection rate of CTwas 50.0% and 70.0%, respectively. Conclusion It is im por-tant to pay attention to the use of com bined im aging exam inations and the follow-up results. In the cas-es of suspicious for rib fracture in axillary section, CTexam ination is suggested in such false X-ray negative cases.

BACKGROUND:The increase of serum cholesterol levels is positively correlated with the increasing incidence of osteoarthritis. Little is known about the role of high-fat diet in the degeneration of articular cartilage and induced arthritis.
OBJECTIVE:To observe the morphology change of the articular cartilage of New Zealand rabbits after high-fat diet, and explore the role of food in the articular cartilage degeneration.
METHODS:Forty New Zealand white rabbits were randomly divided into two groups. In control group, rabbits were fed with basal diet. In high-fat diet group, rabbits were fed with high-fat diet (20%lard and 80%basal diet). Fasting blood samples was taken every 4 weeks, to detect the triglyceride and total cholesterol levels. After rabbit were fed for 28 weeks, the knee joint was grossly observed and the femoral condyle cartilage was scanned by scanning electron microscope.
RESULTS AND CONCLUSION:Compared with the control group, the triglyceride and total cholesterol levels were significantly increased in the high fat group (P<0.05). Under scanning electron microscope, the high-fat group showed rough surface of condylar cartilage, shal ow pouch, messy arrangement, irregular shape and uneven size. The surface of cartilage exhibited focal denudation, irregular arrangement, uneven porosity and fractured cavity. Under higher magnification, smal mountain-like structure of cartilage surface became shal ow and flattened, the pores disappeared. Long-term high-fat diet may induce and aggravate cartilage damage, suggesting it may be involved in the pathogenesis of osteoarthritis.

Cotinine is the best biomarker for the assessment of the exposure to environmental tobacco smoke(ETS).With the"Framework Convention on Tobacco Control"implemented in China,the hazards from ETS are increasingly concerned,the determination of cotinine in human body which reflects the internal exposure to ETS received universal recognition day by day.It is imperative to create a standardized,simple and fast method to detect cotinine in human body.The recent determination techniques of cotinine,the application in different human samples and the direction of development were introduced in the present paper.

Objective To investigate the relationship between p38MAPK and astrocytes activation by detecting the changes of p38MAPK,TNF-?,NO and NOS in the substance P-induced activation of spinal cord astrocytes in vitro.Methods Astrocytes,cultured from spinal cord of SPF rat and identified by immunofluorescence method with GFAP antibody,were grouped into the control group,the SP stimulus group(SP group) and the SB203580 interrupt p38MAPK group(interrupt group) in which SP(10-7 mol/L) and SB203580(10 ?mol/L) were added to the supernatant for 12 h.The WB method,RT-PCR and ELISA were used to determine the changes of p38MAPK,phosphorylated p38MAPK,GFAP mRNA,TNF-?,NO and NOS in the astrocytes or supernatant at 1,3,12,24 and 36 h respectively.Results The GFAP positive rate of the cultured cells was higher than 95%.p38MAPK level in the SP group did not have any change,whereas p-p38 level increased significantly after 1 h and GFAP mRNA,NO,NOS and TNF-? level increased after 3 h.When p38MAPK pathway was inhibited by SB203580 in the SP+SB group,GFAP mRNA,NO,NOS and TNF-? were significantly reduced compared with those in the SP group.The level of p38MAPK,p-p38,GFAP mRNA,NO,NOS and TNF-? in the SB group did not have changes compared with those in the normal group.Conclusion These results indicate that p38MAPK signal pathway contributes to substance P induced activation of spinal cord astrocytes according to inflammatory factors attenuation after p38MAPK signal pathway interruption by SB203580.

Objective To investigate the expression of p38 mitogen-activated protein kinase(p38MAPK) in posterior horn of L5-S2 spinal cord in analgesia for chronic prostate pain with intrathecal injection of SB203580(p38MAPK inhibitor activity) in a murine model.Methods Forty-five male SPF SD rats were randomly divided into control group(n=5),prostatitis group(n=10) and SB203580 analgesia group(n=30,SB203580 was intrathecally injected).In the SB203580 analgesia group,complete Freund's adjuvant was injected into the prostate and SB203580 of 0.5?g/10?l,2.5?g/10?l and 12.5?g/10?l was intrathecally injected respectively.Five days and ten days after drug administration or simple modeling,phosphorylated p38MAPK(p-p38MAPK),glial fibrillary acidic protein(GFAP),tumor necrosis factor-?(TNF-?),nitric oxide(NO) level and induced nitric oxide synthase(iNOS) activity of L5-S2 spinal dorsal horn of rats were determined by Western blotting and ELISA assay,respectively.Results In the prostatitis group,the p-p38MAPK,GFAP,TNF-?,NO level and iNOS activity of L5-S2 spinal dorsal horn of rats at the 5th day and the 10th day were significantly higher than those of the control group and increased with time.The p-p38MAPK,GFAP,TNF-?,NO levels and iNOS activity at the 5th day and 10th day in SB203580 analgesia group were lower than those in the prostatitis group.Conclusion P38MAPK signaling pathway was involved in the mechanism of pain conduction and analgesia by SB203580 after prostatitis.The level of inflammatory factors in spinal cord can be effectively reduced by blocking the p38MAPK signaling pathway.

Objective To deterine the expression of TNF-related apoptosis inducing ligand (TRAIL) and its receptor DR5 in murine myocardium with acute viral myocarditis(VM).Methods We builted the model of VM.Eight mice of the VM group and the normal control group were sacrificed on the 7th,10th,14th,21st,28th day after inoculation CVB3 virus.The myocardial histopathological scores were counted.The terminal reansferase-mediated dUPT-biotin nick end-labeling (TUNEL) assays was used to quantified apoptosis rate.The expression of TRAIL and DR5 protein and mRNA were determined by the method of immunohistochemistry and reverse-transcription polymerase chain reaction (RT-PCR).Results The expression of TRAIL and DR5 protein and mRNA were found in myocardium of both the normal control group and the VM group.The expression of TRAIL protein of the VM group(14th) were significantly higher than which in the normal group at the same time and the VM group(7th)(F=9.17,P<0.01).The DR5 protein of the VM group(10th,14th,21st)were significantly higher than which in the normal group at the same time and the VM group(7th)(F=13.32,P<0.01).The expression of TRAIL and DR5 protein were positive correlated with the myocardial histopathological scores and the apoptosis rate.The expression of TRAIL mRNA of the VM group(10th) were significantly higher than which in the normal group at the same time and the VM group(7th)(F=10.86,P<0.01).The DR5 mRNA of the VM group(10th,14th)were significantly higher than which in the normal group at the same time and the VM group(7th)(F=22.75,P<0.01).Conclusion High characteristic expressions of protein/mRNA TRAIL and DR5 were observed in the myocardium of mice with VM.The level was positive correlationed with the account of pathology and the rate of apoptosis.The apoptosis induced by TRAIL and DR5 may participate in the pathophysiological processes of VM.

Objective To summarize the experience on bronchiallavage to cure pulmonary atelectasis and pulmonary infection after thoracotomy.Methods 48 patients were selected and treated by bronchiallavage under monitoring,and antibiotics was used according to sputum culture and drug sensitive test.The out come was analyzed and evaluated.Results All patients of the pulmonary atelectasis were cured and those with pulmonary infection were controled.There was no serious complication.Conclusion Bronchiallavage is applied to treat pulmonary atelectasis and pulmonary infection after thoracotomy,the curative rate is significant.The appliance of antibiotics is guided by drug sensitivity.It is such a safe and feasible therapeutic tool that the bronchofibroscope is applied to cure pulmonary atelectasis and pulmonary infection after thoracotomy.