Objective To explore the role of plasma level of homocysteine ( Hey ) in progressive ischemic stroke (PIS)of carotid artery system and to investigate the relationship between Hcy and carotid artery atherosclerosis(AS).Methods Plasma Hcy concentrations were measured in 68 patients with progressive ischemic stroke of carotid artery system (PIS group)and 72 patients with non-progressive ischemic stroke of carotid artery system ( non-PIS group ).Plasma Hcy level and carotid artery intima-media thickness ( IMT ) were compared among PIS group and non-PIS group as well as normal control group.The linear correlation between plasma Hcy level and IMT in PIS group was analyzed.Results Plasma Hcy concentrations was respectively (22.52 ± 5.26) μmol/L,( 16.38 ± 4.23) μmol/L and( 10.13 ± 2.16) μmol/L in PIS group,non-PIS group and normal control group( F =56.87,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant ( P ＜ 0.01 ).And there was also significant difference on plasma Hcy levels between PIS group and non-PIS group( P ＜ 0.01 ).The thickness of carotid artery IMT was respectively(1.12 ± 0.34)mm,(0.96 ± 0.27)mm and(0.43 ± 0.21 )mm in PIS group,non-PIS group and normal control group( F =28.47,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant( P ＜ 0.01 ).And there was also significant difference on IMT between PIS group and non-PIS group ( P ＜ 0.01 ).In PIS group plasma Hcy level was positively correlated with the carotid artery IMT ( r =0.687,P ＜ 0.01 ).Conclusion The plasma Hcy level is closely related to the occurrence and development of carotid artery atherosclerosis and PIS of carotid artery system,detection of plasma Hcy level helps to predict the occurrence of PIS of carotid artery system and provide an evidence for the therapy decision.

Objective: To observe the effect of acupuncture around the diseased region combined with point injection on post-herpetic neuralgia. Methods: Point injection on 30 cases in the related segments of spinal cord after needling around the diseased region. Results: Twenty-seven cases got effect and the total effect rate was 90%. Conclusion: Acupuncture combined with point injection has remarkable curative effect on post-herpetic neuralgia.

Objective To investigate the correlation of smokers in wnt/β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.Methods In the Department of Respiratory Medicine of the People's Hospital of Wuhan University,30 cases lung squamous cell carcinoma patients with a history of smoking,30 cases lung squamous cell carcinoma patients with no history of smoking,30 cases bronchiectasis patients with a history of smoking,30 cases bronchiectasis patients with no history of smoking were chose respectively as A group,B group,C group and D group.After the patients were treated by surgery,the removal of diseased tissue used immunohistochemical staining for CD68 + testing,TNF-α,wnt-2,β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method,then wnt-2,β-catenin,TCF-4 transcription of mRNA concentrations were detected by using RT-PCR method.Results Immunohistochemical studies showed that cells in TNF-α,CD68 + expression of A group,B group,C group and D group were respectively 0.895±0.036 and 0.931±0.237,0.125±0.018 and 0.631±0.064,0.083±0.042 and 0.311±0.062,0.053±0.004 and 0.196±0.020,the differences were statistically significant(P＜0.05).Westblot test Results showed that wnt-2,β-catenin,TCF-4 expression level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05),testing of RT-PCR showed that wnt-2,β-catenin,TCF-4 translation level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05).Conclusion Smoking for body wnt/β-catenin pathway has a cell activation,after cigarette activate wnt/β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt/β-catenin signaling transduction circumstances.The detection of wnt/beta-catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.

Physiological pacing has undergone three stages: atrioventricular sequential pacing,chronotropic pacing and cardiac resynchronization therapy.How to make pacing therapy more physiological,so as to meet the physiological needs of patients,is the unremitting pursuit of clinicians.With further understanding of cardiac function and the development of pacemaker manufacturing technology,pacing function will become more and more physiological.At the same time,the newer generation of pacemakers will have more sophisticated program control function,higher automation,simulation of the doctor's thinking,and the pacing parameters can be optimized automatically after implantation allowing pacemaker to operate in the most effective and energy-saving state.From cardiac rhythm managers to heart disease managers,their unique efficacy will make physiological pacing function perfect day by day.In this paper,the research progress in this field is analyzed,which aims to provide the basis for pacing mode,pacing site and pacing interval in the future.

Objective To discuss the clinical efficacy of warm acupuncture, acupoint injection, bloodletting and electroacupuncture, and their interaction effects for acute cold type facial paralysis. Methods Totally of 48 patients with acute cold type facial paralysis were randomly divided into three groups by orthogonal experimental design, and then were assigned to 16 treatment plans. All patients received routine basic treatment, and then were given warm acupuncture, acupoint injection, bloodletting and electroacupuncture according to assigning results. 10 d were as a treatment course, with 2 d as interval, 3 treatment courses in total. The neurological function scores before and after treatment and facial nerve recovery time were recorded. Results There was statistical significance in scores of facial nerve function, warm acupuncture, acupoint injection, electroacupuncture, bloodletting had significant difference with errorterm (P<0.01). There was statistical significance in the recovery time of the facial nerve function, warm acupuncture, bloodletting compared with error term (P<0.01). Conclusion Warm acupuncture, acupoint injection, bloodletting and electroacupuncture combine with acupuncture are better for acute cold type facial paralysis. Warm acupuncture and bloodletting therapy in the acute phase of interventional therapy can shorten the recovery time of facial nerve.

Objective Toexploretheclinicaldiagnosisvalueofthehighfrequencycolordopplerultrasound(HFCDU)character-istiesofwristdamageinpatientswithrheumatoidarthritis(RA).Methods 78patientswithRA(RAgroup)werecollectedfrom June 2012 to December 2012 in the Affiliated Hospital of Inner Mongolia Medical University ,sonogram anatomy changes and syno-vial thickness of bilateral wrist were measured using colorul trasound ,meanwhile the respective data obtained from 50 healthy peo-ple undergoing physical examination(healthy control group);using color doppler flow imaging and color doppler energy ,synovial blood flow of artery resistance index (RI) and pulse index(PI) in synovial were measured and detected according to semi-quantitative method of alder ,and explord the correlations between RI and PI .Results (1)Among the 78 cases(156 joints) with RA ,join thy-drops(65 cases ,122 joints) ,inflammatory exudation(34 cases ,70 joints) ,synovium proliferation(78 cases ,153 joints) ,pannus(37 cases ,69 joints) and bony erosion(29 cases ,58 joints) were found.The thickness of synovial membrane of wrist in RA group was (3 .50 ± 1 .37)mm ,that in healthy control group was(1 .46 ± 0 .32)mm ,the different was statistically significant(P<0 .05) .(2) 64 cases among the 78 patients with RA were detected blood flow signals ,RI progressively reduced with blood flow grade the increas-ion which there was a negative correlation (r= -0 .79 ,P<0 .05);there was no significant correlation between PI and blood flow grade .(3) Ultrasound examination was more sensitive than rheumatoid factor (RF) and X-ray in detection rate of RA (P<0 .05) . Conclusion Ultrasound examination could detecte the pathological changes of wrist in patients with RA which is more sensitive than RF and X-ray ,and it could improve the detection rate of RA which contribute to early diagnosis and reasonable treatment for RA .

Nuclear factor erythroid2-related factor 2(Nrf2) is an important protective transcription factor that generally exists in various organisms and cellules.Normally,it is sequestered in the cytoplasm.Environmental stress can activate its signal factor,lead to its nuclear translocation,and enhance the anti-oxidation,deintoxication and anti-apoptosis function of the organism,for which the Nrf2-antioxidant response element(Nrf2-ARE) signaling pathway is one of the most important protective ones.This article updates the cytoprotective effect of Nrf2-ARE signaling pathway.

This review summarized the biological activities of natural saponins,introduced its action mechanisms,clinical applications and research progresses in cardiovascular system,antitumor,immunological enhancement,blood sugar suppression,contraception,antifugal and insecticide,etc,and prospected its exploitations.

Objective To explore the effect of osteopontin(OPN) as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence.Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer(observation group) and 22 cases of normal subjects(control group);semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues.The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed.Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group(P

Objective To observe the effect of medicine-induced posterior vitreous detachment (PVD) on proliferative vitreoretinopathy (PVR).Methods.PVR was induced in the left eyes of 24 pigmented rabbits by intravitreal injection with platelet rich plasma.The rabbits were randomly divided into two experimental groups (group A and B) and one control group with 8 eyes in each group.Three hours later,the eyes in group A and B and the control group underwent intravireal injection with 1 U plasmin 0.05 ml+ 20 U hyaluronidase 0.05 ml,plasmin 0.1 ml,and balance salt solution 0.1 ml,respectively.The grade of PVR was recorded 1,7,and 28 days after the intravitreal injection,and the eyes were examined by flash electroretinogram (FERG),B-scan,and retinal histopathological examination.Results The PVR models of rabbit eyes were induced successfully.On the 7th day after injection,complete and partial PVD was found in 5 and 3 eyes respectively in group A;partial PVD in 5 eyes and no complete PVD was observed in group B;there was no PVD in the other 3 eyes in group B and also in the eyes in the control group.On the 28th day after intravitreal injection,PVR grade of group A and B were both obviously lower than that of the control group(D= 75.6,98.9;P = 0.003,P = 0.011) ;On the 7th and 28th day after injection,the b-wave amplitude in group A and B was significantly higher than that in the control group;PVR grade of the PVD eyes was lower than that of non-PVD eyes;PVR grade of the complete PVD eyes was only 0 ～ 1.Conclusions Three hours after the PVR models of rabbit eyes were induced,complete PVD induced by intravitreal injection of plasmin combined with hyaluronidase could prevent the development of PVR of rabbit eyes in some degree;partial PVD induced by plasmin alone or combined with hyaluronidase could relieve the development of PVR.