Study was performed on 100 serum samples of patients (15-70 aged) treated of gastro-duodenal diseases and 31 healthy persons by Immunoblot assay. The results showed that: the prevalence of H.pylori positive in patients with gastric ulcer, duodenal ulcer and gastritis was 76.5, 95.8 and 73%, respectively. The prevalence of H. pylori seropositive was found 64.5% in healthy persons. The prevalence of anti-CagA and anti-VacA antibodies was 86.2% and 60.6% (respectively) in patients and was 70% and 40% (respectively) in healthy person.
Antigens, Bacterial ; Bacterial Proteins ; Helicobacter pylori ; Antibodies ;

Fracture of the shaft of femur and tibia in children aged from 4 to 17 sometimes caused the problem in reduction and immobilization and needed an osteosynthesis. Among the internal fixation procedures, intramedullary nailing by using Metaizeau’s flexible wire under C-arm control showed the best way and the best choice. 30 fractures of the shaft of femur and tibia failed in conservative treatment have been operated by above methods with successful results since Jan-1996 to February-2000 in Saint-Paul hospital. The advantages of the method were noted as no opening fracture focus, no bleeding, no risk of infection, short stay in hospital and no need of cast immobilization. So patients will come back soon to theirs activities and school.
Hepatitis A virus ; Serologic Tests

To study the proportion of H. pylori infection in classes of patients with gastroduodenal ulcer, 4 methods were used simultaneously: culture, urease test, Gram stain and histology. The results found that the proportion of H. pylori infection is highest in duodenal ulcer or duodenal ulcer combining with gastritis, gastric ulcer combining with duodenitis (91.7% - 100%). In gastric ulcer or gastritis only, the positive proportion is 54-65% by culture, 54-56% by urease test, 63-82% by Gram stain, 37-52% by histology
Helicobacter pylori ; Peptic Ulcer

There were 296 cases of typhoid fever of which 52 cases admitted in hospital and no death happened in a training camp for new soldiers in 55 days outbreak. Investigated the outbreak situation at local health station and outbreak area: house, dining room, kitchen, canteen, toilet, resources of water, dung and waste. The improper dung management caused environmental pollution was the main cause of outbreak. Clinical condition was a typical and treated by common and special antibiotics had good results.
Typhoid Fever ; Military Personnel ; Disease Outbreaks

Background: Facing urgent requirements of the rapid diagnosis of tuberculosis nowadays, our laboratory have improved the multiple PCR for 3 target genes spontaneously (IS 1081, IS 6110, 23S rDNA) and developed into the multiplex PCR kit to improve the effects of diagnosis of tuberculosis. Objective: To evaluate the multiplex PCR kit for the 3 target genes (IS 1081, IS 6110, 23S rDNA) for the diagnosis of tuberculosis on standard panel and clinical specimens. Subjects and method: DNA extracted from M. tuberculosis strains and clinical specimens were amplified by multiplex PCR kit, comparison with singleplex PCR for each target gene. Results: On standard panel, sensitivity and specificity reached 100% and detected limitation was 100 fg DNA template (about 3 M. tuberculosis cells). Multiplex PCR kit detected 195/307 (63.52%) suspected clinical specimens compared with singleplex PCR which detected 106/307 (34.53%) for IS 1081, 182/307 (59.82%) for IS 6110 and 136/307 (44.30%) for 23S rDNA. Conclusions: PCR amplification with one target gene was insufficient for diagnosis of the tuberculosis strains in Viet Nam. Multiplex PCR kit for 3 target genes simultaneously (IS 1081, IS 6110, 23S rDNA) could detect all tuberculosis strains.
Tuberculosis ; PCR

Introduction: Mycobacterium tuberculosis resists rifampicin (RIF) because of mutations in the rpoB (the p subunit of RNA polymerase) gene, mostly in the 81 bp region. \r\n', u'Objectives: Identify the frequency and characteristics relative to drug - resistant rpoB gene mutation in RIF - resistant M. tuberculosis strains. \r\n', u'Subjects and method: 40 M. tuberculosis strains including 11 RIF - sensitive strains and 29 RIF - resistant strains. Some bio molecular techniques were used such as extracting mycobacterial DNA, PCR, cloning, sequencing and analyzing mutation related RIF - resistance on rpoB gene. \r\n', u'Results: No mutation was found on the 81 bp region of rpoB gene of the RIF - sensitive M. tuberculosis strains. The rate of mutation on rpoB gene of 29 RIF - resistant M. tuberculosis strains is 96.6%. We found 12 mutation codon positions on the 81 bp region of the rpoB gene, and the mutation codon positions with high frequency were 531 (51.7%) and 526 (31%). The mutation position found in only one strain is codon 519 (3.4%) but not found in other reports. There are 15 types of drug resistant mutations in which TCG531 TCG is the most common with 50%. Multi - drug resistance was seen in mutable and none mutable cases, with all codon positions and mutable forms. \r\n', u'Conclusion: No mutation was found on the 81 bp region of the rpoB gene of RtF - sensitive M. tuberculosis strains. The rate of mutation on the rpoB gene of RIF - resistant M. tuberculosis strains is 96.6%. The new mutation position found is codon 519. The mutation on the rpoB gene does not determine the multi - drug resistance of M. tuberculosis. \r\n', u'
Mutation ; rpoB gene ; Rifampicin - resistant M. tuberculosis

Background: The combination medical between army and pepople in protection, healthcare for people and army in the whole country was a great policy of The Party and State. Objectives: Evaluate result of combination between armed forces in epidemic control and preventive medicine programs at mountainous and coastal areas of Thua Thien Hue in 2006. Studying knowledge, attitude, practice of army medical officers and men canvassed masses about epidemic control and preventive medicine programs. Subjects and method: Officers, men, army medical officers in 41 communes (28 coastal communes and 13 mountainous communes) of 5 district have combination medical between army and pepople. Using epidemiology method to description, analysis, cross-sectional study. Results: In 2006, home-flies spraying was carried out in 18 out of 20 markets; 1,030 all size restaurants and 18 residential areas of 5 districts. The number of chemically treated latrines in high epidemic areas was accounted fof 15,1%. A population-based latrine construction project was initiated to build 838 new latrines, contributing to minimize the invironmental pollution. 9,17% of waterwells and water tanks were hygienically treated; 11,434 people used clean water source; and 46,436 people washed their hands with soap. Studying knowledge, attitude, practice on preventive medicine programs of army medical officers showed very good results. Conclusion: The combination medical between army and pepople in preventive medicine, epidemic control gave wide efficiency for mountainous and coastal communes of Thua Thien Hue. It actively supported for Health station of 28 coastal communes and 13 mountainous communes in preventive medicine work.
Community Health Services/ ethics ; manpower ; Health Knowledge ; Attitudes ; Practice ;

Background: Hypertensive with impaired fasting glucose is the basic expression of metabolic syndrome. Objectives: To study the IR and the correlation between Fasting Plasma Insulin (FPI) and Fasting Plasma Glucose (FPG) concentration in hypertensive patients with Impaired Fasting Glucose (IFG). Subjects and method: The descriptive, cross-sectional study was carried out on 38 hypertensive patients with IFG (IFG group) and 30 hypertensive patients without IFG (control group). The data were collected and analyzed by SPSS 11.5 software. Results:The FPI and IR index in the IFG group were 14.6+/-5.2 micro mol/ml and 3. 94+/- l.40, respectively, higher than in the control group (9.6+/-4.2 micro mol/ml and 2.17+/-0.99/ respectively) with p<0.00l. There was a positive correlation between FPI and FPG systolic blood and diastolic blood pressure/ with correlation coefficients were 0.4/ 0.48 and 0.46/ respectively (p<0.00l). Conclusion: There was an increase of FPI and IR index in the hypertensive patients with IFG compared to those without IFG. \r\n', u'\r\n', u'
Insulin resistance ; hypertensive patients ; impaired fasting glucose.

Background: HIV/AIDS pandemic really become danger to mankind on the earth. Objectives: Description epidemiological characteristics of HIV/AIDS patients. Studying clinical expression, learning aboutdemands of infected patients and community care for infected patients. Subjects and method: 71 HIV/AIDS people in Thua Thien Hue were studied in 2006. Using descriptive method to give epidemiological characteristics and behaviour of HIV/AIDS people. People were determined HIV/AIDS infection based on the criteria of Ministry of Health. The information were collected by target study. Results: HIV/AIDS people mainly from the age of 20 to 39 (83,10%), males were 52,10% and in various levels of education and all kinds of occupation. The rate of sexually transmitted infection was 84,5% and infection transmission was 15,5%. The rate of sexually transmitted infection within the last 12 months was 57,7%. 63,4% of infected people used condom while having sex with their spouses or partners. 1,4% of infected people using drug infection within the last month. 38% of the infected people had manifestations of AIDS, 77,8% of HIV/AIDS were treated with antiviral drugs. Families and communities had positive attitudes and behaviors to the HIV/AIDS people. Infected people being remoted from the society was 12,7%, 88,7% of them receiving supports from their communities such as disease treatment, spirit and material supports and job opportunities. Conclusion: Strengthen communication activities to change behavior of HIV/AIDS people and in communitiy to reduce alienation, discrimination for infected people. Execute socialization in care and support for HIV/AIDS people.
HIV/ immunology ; isolation & ; purification ; pathogenicity ; Community Health Services/ organization & ; administration ; Epidemiologic Methods ;

Background: In 24 years, Expanded Programe on Immunization\r\n', u'(EPI) at Thua Thien Hue province achieve high efficiency, reduce remarkably infection rate of children disease in EPI. Objectives: Assessment of instruments and cold chain at City, District Health Center and Commune Health station. Assessment of knowledge and practice EPI of medical officers in districts and communes. Subjects and method: Instrument, cold chain system and officers at City, District Health Center and Commune Health station. Method: Cross-section descriptive study; Observe instruments and cold chain at Health stations and fill in available forms. Interview medical officers, observe practical manipulation and fill in available forms. Results: The rate of good knowledge varied from 61,64% to 94,55% and the rate of appropriate practice was from 45,70% to 80,92%. On average, each commune health station had 0,421 refrigeration; 0,128 ice cabinet; 0,258 cold box; 2,259 thermoses; 6,623 ice packs; 2,826 thermometers and11,321 safe boxes. All commune health stations have vaccine containing thermos; one station has no thermometer; two have no safe box and five have no ice pack. Conclusion: All commune health stations have essential instruments, cold chain. Very few health station lack of one or some types. Medical officers almost have basic knowledge about expandedimmunization, the rate of answering right theoretical questions from 61,64% to 94,55%. Practical manipulation had still many errors, rate of manipulation right only 45,70% to 80,92%. District officers manipulated right higher than commune officers.\r\n', u'
Refrigeration/ instrumentation ; Vaccination/ instrumentation ; mortality ; methods ; Health Knowledge ; Attitudes ; Practice ;