Objective This study was undertaken to survey positive rates for rubella antibody of pregnant women in Wuhu and to explore possible related factors. Methods A questionnaire was used to collect information of these women, the levels of rubella IgG antibody was determined by enzyme-linked immunosorbent assay and the positive rates for rubella antibody in pregnant women were surveyed. Results This study showed a high seroprevalence of about 74.29% among non-immune women while the rubella vaccine coverage can be ignored. There was no significant difference in positive rates for rubella antibody between different education, average family income and age. Conclusions The persistence of the wild virus is high. The prevention campaigns and the efforts begun to vaccine all the non-immune women of childbearing age must be pursued.