1.Study on HPLC Fingerprint of Total Flavonoids from Pollen ofBrassica campestrisL.
Chengcheng FENG ; Sude YANG ; Shaowei YUAN ; Zhenzhong WANG ; Yuan BI ; Siyang FAN ; Yifang YANG ; Wei XIAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):591-595
In this article, HPLC fingerprint analysis method for total flavonoids from pollen of Brassica campestris L.was established., The HPLC fingerprint was performed on Waters C18 column (250 mm × 4.6 mm, 5 μm), eluted gradiently with the mixture of acetonitrile and 0.4% phosphoric acid aqueous solution at a flow rate of 0.8 mL·min-1. The column temperature was 40℃. The detection wavelength was 320 nm. The HPLC standard fingerprint of total flavonoids from pollen of Brassica campestrisL. was established, and 16 common peaks were calibrated. The method was simple, stable, and reproducible. It could be applied for quality control of total flavonoids from pollen of Brassica campestris L.
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