Objective To investigate the effect of transcranial direct current stimulation combined with motor relearning program on the recovery of upper limb motor function after stroke.Methods From September 2015 to June 2016,40 patients with first-ever stroke received rehabilitation therapy at the Department of Rehabilitation Medicine,Xuanwu Hospital,Capital Medical University were enrolled prospectively.Their course of disease was 1 to 6 months.They were divided into either a combined treatment group or a control group according to the odd and even numbers in hospital (n =20 in each group).The patients of both groups received traditional rehabilitation training and motor relearning program for upper lindb recovery,2 times a day,once for 30 min,5 days a week for 6 weeks.The combined treatment group was also given transcranial direct current stimulation,2 times a day,once for 20 min,5 days a week,while the control group was only treated with sham stimulation treatment.The patients were assessed before treatment and at 6 weeks after treatment.Fugl-Meyer Assessment (FMA) scores and Action Research Arm Test (ARAT) were used to assess upper limb motor function.The modified Barthel index (MBI) was used to evaluate the activities of daily living.Results There was no significant difference in the observation indices before treatment between the 2 groups (P ＞ 0.05).Six weeks after treatment,the differences of the upper limb FMA score and ARAT score before treatment were superior to the control group,they were 13 ± 3,10 ± 3 and 10 ± 2,8 ± 2,respectively (t =3.503 and 3.244 respectively,all P ＜ 0.01).There was no significant difference in the MBI score between the 2 groups (P ＞ 0.05).Conclusion Transcranial direct current stimulation combined with motor relearning program may effectively improve the upper limb motor function in patients with stroke and promote the improvement of the activities of daily living.

Objective To study the drug retention of HPC MMC in bladder after intravesical instillation. Methods The pharmacokinetics of the intravesical mucous adhesive anticancer agent HPC MMC in urine and in tissue were comparatively studied with conventional mitomycin C aqueous solution(S MMC) by means of animal experiment. Results After instillation, the half life of MMC in urine in the 2 groups was 2.61 h and 0.56 h and in tissue 2.33h and 0.46 h respectively. Conclusions Suggesting that HPC MMC remained longer within the urinary bladder. It may be therapeutically more efficient.

BACKGROUND:In the orthodontic treatment, double wire technique is commonly used to correct the torque of anterior teeth;however, the underlying mechanical mechanisms remain unclear. OBJECTIVE:To study the torque capabilities of DamonQ system through the three-dimensional finite element method. METHODS:Three-dimensional finite element models of Damon Q brackets with different dimension arch wires were established and were grouped according to arch wire with different dimensions. The torque capabilities of Damon Q system with different dimensions and materials arch wire were analyzed. RESULTS AND CONCLUSION:The Damon Q brackets with single or double wires had the same variation of torque, torque angle curve changes under the condition of different size and material arch wires. Torque moment increased gradually with increasing torque angle. Rectangular-round double wires produced larger torque moment compared with single rectangular wire, whereas double round wires produced smal er torque moment compared with single rectangular wire. The torque moment produced by rectangular-round double wires or by single rectangular wire was approximately 2-8 times or 1-5 times more than that by double round wires. The torque moment that rectangular-round double wires produced was larger than that single wire produced in the DamonQ system. In the early clinical practice, we can use double round wires to control root movement. In the middle-term of the treatment, we can use rectangular-round double wires to control root movement as the mild forces produced by them reduce root absorption. This study lays a foundation for the application of Damon Q bracket with double wire technology in the clinic.

Objective:To express fusion protein of mouse thymic stromal lymphopoietin (TSLP) and HIV-1 gp120BAL V1/V2 subdomain in 293F cell.Methods:Full length of the V1V2 sequence from BAL isolate was fused with the C-terminus of mouse thymic stromal lymphopoietin (TSLP) and sub-cloned into pCEP-Pu vector to construct the eukaryotic expression plasmid-pCTV1V2BAL.The recombinant plasmid was confirmed by enzyme digestion and sequencing , then transfected into 293 F cells using PEI as a transfection reagent .The fusion protein was purified by metal chelate affinity chromatography and characterized by SDS -PAGE and Western blot . The epitopes of V1/V2 in fusion protein were identified by ELISA .Results:The SDS-PAGE and Western blot results showed that there were highly heterogeneous glycoprotein bands at the site between 35 kD and 55 kD, which reacted with anti-mTSLP rabbit polyclonal antibody and anti-His tag mouse monoclonal antibody .The ELISA analysis showed that antibodies to V 1/V2BAL existed in the sera of HIV-1 positive patients.Conclusion:The mTSLP-V1/V2 fusion protein was successfully expressed in 293F cells, which may be useful for HIV-1 vaccine research .

Objective To investigate the effect of cathodal transcranial direct current stimulation (ctDCS)on upper limb motor function in patients with stroke. Methods From July 2016 to February 2017,45 patients with first-ever stroke hemiplegia treated with rehabilitation at the Department of Rehabilitation Medicine,Xuanwu Hospital,Capital Medical University were enrolled prospectively. Their course of disease was 1 -6 months. They were randomly divided into a 1. 0 mA group,a 2. 0 mA group,and a control group (n =15 in each group)according to the random number table. The 3 groups of patients received traditional rehabilitation training,2 times a day,once for 45 min,5 days a week for 2 weeks. On this basis,the 1. 0 mA group and 2. 0 mA group were treated with 1. 0 mA and 2. 0 mA ctDCS for two weeks,once a day,once for 20 min,5 days a week at the same time,while the control group was only treated with sham stimulation. The upper limb function was evaluated before treatment and 2 weeks after treatment. Fugl-Meyer Assessment (FMA)score and Action Research Arm Test (ARAT)were used to assess the upper limb motor function. The modified Barthel index (MBI)was used to evaluate the activities of daily living. Paired t test was used to compare before and after treatment in the groups. Single factor analysis of variance was used to compare between the groups. Results (1)There were no significant differences in the upper limb FMA score, ARAT score,and MBI score before treatment among the three groups of patients (all P >0. 05). Two weeks after treatment,the upper limb FMA score,ARAT score,and MBI score of the patients in the 3 groups were significantly improved compared with those in the same groups before treatment. The differences in the 1. 0 mA group were 12 ±3,10 ±2,and 22 ±9,respectively,those in the 2. 0 mA group were 12 ±3,10 ±3, and 20 ±6,respectively,and those in the control group were 9 ±3,7 ±3,and 18 ±7,respectively in control group (all P <0. 01). (2)Two weeks after treatment,the upper limb FMA scores and ARAT scores in the 1. 0 mA group and 2. 0 mA group were superior to the control group (the FMA scores all P < 0. 01 and ARAT scores all P <0. 05),while there was no significant difference in scores between the 1. 0 mA group and 2. 0 mA group (P > 0. 05). The were no significant differences in the MBI scores among the three groups (P >0. 05). Conclusion Cathodal transcranial direct current stimulation may have a trend of improving the upper limb motor function and promote the improvement of activity of daily living in patients with stroke,however,there was no significant difference in treatment effect of ctDCS intensity between the 1. 0 mA and 2. 0 mA treatment.

Objective:To investigate any effect of combining mirror visual feedback (MVF) training with robot-assisted gait training (RGT) in promoting the recovery of lower limb motor function among stroke survivors.Methods:Sixty stroke survivors were randomly divided into a combined treatment group, an RGT group and a control group, each of 20. In addition to traditional rehabilitation, the RGT group received 30min of RGT 5 days a week for 4 weeks, while the combined treatment group underwent 30min of RGT and also 20min of MVF training with the same frequency and duration. Before and after the treatment, the lower extremities motor functioning and walking ability of all of the patients were assessed using the Fugl-Meyer Assessment for the lower extremities (FMA-LE), the Berg Balance Scale (BBS) and Functional Ambulation Categories (FAC).Results:There were no systematic differences in any of the measurements before the treatment. After the 4 weeks, however, the average FMA-LE, BBS and FAC scores of the combined treatment and RGT groups were significantly better than the control group′s averages, and those of the combined treatment group were significantly superior to the RGT group′s. All of the groups had, however, improved significantly compared with before the treatment.Conclusions:Robot-assisted gait training is more effective in enhancing the motor functioning of the lower extremities and walking ability when it is combined with MVF.

Objective To explore the relationship between serum heme oxygenase-1(HO-1),CD39 levels and cardiac function and major adverse cardiovascular events(MACE)in patients with successful cardiopulmo-nary resuscitation.Methods A total of 160 patients admitted to the Zigong First People's Hospital from De-cember 2019 to December 2022 who underwent successful cardiopulmonary resuscitation due to sudden heart-beat and respiratory arrest were selected as the study group.The study group was divided into cardiac function grade Ⅱ group,cardiac function grade Ⅲ group and cardiac function grade Ⅳ group using the New York Heart Association(NYHA)cardiac function grading method.In addition,100 healthy subjects who underwent physical examination in the same period were selected as the control group.The levels of serum HO-1 and CD39 in two groups were detected.After 6 months of follow-up,there were 91 cases in non-MACE group and 69 cases in MACE group.The predictive value of serum HO-1 and CD39 levels for MACE in patients with successful cardiopulmonary resuscitation was evaluated by receiver operating characteristic curve.Binary Lo-gistic stepwise regression analysis was used to investigate the influencing factors of MACE in patients with successful cardiopulmonary resuscitation.Results The serum levels of HO-1 and CD39 in the study group were higher than those in the control group,and the difference was statistically significant(P＜0.05).The levels of serum HO-1 and CD39 in cardiac function grade Ⅳ group were higher than those in cardiac function grade Ⅲ group and cardiac function grade Ⅱ group,and the difference was statistically significant(P＜0.05).Spearman correlation analysis showed that serum HO-1 and CD39 levels were positively correlated with NY-HA cardiac function classification in patients with successful cardiopulmonary resuscitation(r=0.534,0.496,P＜0.001).The serum levels of HO-1 and CD39 in MACE group were higher than those in non-MACE group,and the difference was statistically significant(P＜0.05).The area under the curve(AUC)of serum HO-1 and CD39 to predict MACE in patients with successful CPR were 0.725(95％CI:0.682-0.774)and 0.864(95％CI:0.814-0.917),respectively,and the AUC of the combined prediction was 0.901(95％CI:0.851-0.946).The time from cardiac arrest to resuscitation in non-MACE group was lower than that in MACE group,and the left ventricular ejection fraction(LVEF)was higher than that in MACE group,the difference was statistically significant(P＜0.05).Binary Logistic stepwise regression analysis showed that LVEF＜50％(OR=3.466,95％CI:1.537-7.818),serum HO-1≥5.13 ng/mL(OR=3.804,95％CI:1.706-8.479)and serum CD39≥29.87％(OR=4.345,95％CI:1.852-10.192)were the risk factors for MACE in patients with successful cardiopulmonary resuscitation(P＜0.05).Conclusion Serum HO-1 and CD39 levels have high predictive value for MACE in patients with successful cardiopulmonary resuscitation.

ObjectiveTo study the effect of anodal transcranial direct current stimulation (atDCS) combined with mirror therapy (MT) on upper limb function for stroke patients. MethodsFrom July, 2018 to June, 2019, 54 stroke patients from the Department of Rehabilitation Medicine, Xuanwu Hospital, Capital Medical University, were randomly divided into groups A, B and C, with 18 cases in each group. All the patients received routine medication and rehabilitation training, while group A accepted 60 minutes of MT, group B accepted 20 minuts of atDCS first and then 40 minuts of MT, and group C accepted 40 minutes of MT first and then 20 minuts of atDCS in addittion; for four weeks. They were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE), Action Research Arm Test (ARAT), Motor Assessment Scale (MAS) and modified Barthel Index (MBI) before and after treatment. ResultsThe main effect of time was significant in scores of FMA-UE (F = 159.811, P < 0.001), ARAT (F = 353.227, P < 0.001), MAS (F = 513.494, P < 0.001) and MBI (F = 85.982, P < 0.001), and all of them improved after treatment. The main effect of groups was significant in scores of FMA-UE (F = 12.502, P < 0.001), ARAT (F = 20.095, P < 0.001), MAS (F = 16.371, P < 0.001) and MBI (F = 11.882, P < 0.001). The interacted-effect between time and groups was significant in scores of FMA-UE (F = 37.659, P < 0.001), ARAT (F = 78.681, P < 0.001), MAS (F = 97.997, P < 0.001) and MBI (F = 48.015, P < 0.001); while all the scores were the best in group B (P < 0.01), and there was no significant difference between groups A and C (P > 0.05). ConclusionThe combination of atDCS first and then MT can promote the recovery of motor function of the affected limbs for stroke patients.

Objective To explore the differences between baduanjin and ninth set of broadcast gymnastics in their own pulse parameters before and after intervention for 12 weeks,and to provide objective indicators for the function of Baduanjin from the perspective of pulse diagnosis.Methods 60 healthy subjects were recruited,and 50 subjects were finally included according to the dropout criteria.The subjects were divided into the experimental group and the control group by the random number table method,and different interventions were adopted.Then,the pulse diagnosis of the two groups of subjects was collected,and the impact of the interventions on the viscera was judged by analyzing the Pulse Graph Parameters and contribution.Results The characteristic parameters of the experimental group include 8 time domain parameters(left Chi,left Guan,right Cun,etc.)and 9 frequency domain parameters(left Guan,left Chi,right Guan,etc.),which are mainly distributed in the left Cun,left Guan,left Chi,and right Guan;the characteristic parameters of the control group included 6 time domain parameters(left Cun,left Guan,right Chi,etc.)and 11 frequency parameters(left Guan,left Chi,right Guan,etc.),which were mainly distributed in left Chi,right Chi,and left Guan.Conclusion Practicing Baduanjin can significantly affect pulse diagram,and the accuracy and specificity after intervention are higher than those of the control group.Baduanjin has a certain influence on the circulation of qi and blood in the human meridians,and its mechanism of action may be related to affecting the overall circulation of qi and blood.In the experimental group,Baduanjin had a significant effect on the heart,liver,gallbladder,and kidneys,while the control group had the most obvious changes in the kidneys.For the overall intervention and coordination of the viscera,the Baduanjin group had better effects than the ninth broadcast gymnastics group.

Objective To observe the relationship between functional connectivity(FC)of frontoparietal network(FPN)and cognitive function in patients with cerebral small vessel disease(CSVD)using resting-state functional MRI(rs-fMRI).Methods rs-fMRI of 50 CSVD patients with cognitive impairment(CI group),65 CSVD patients with normal cognition(NC group)and 60 healthy controls(HC group),as well as outcomes of neuropsychological tests were retrospectively analyzed.Brain regions with different FC of FPN were compared among 3 groups and between each 2 groups.Partial correlation analysis was used to evaluate the correlations of FC of brain regions value being statistically different between CI and NC groups and cognitive scores.Results Significant differences of FC in bilateral cingulate gyrus,left middle frontal gyrus,right supramarginal gyrus,right inferior parietal lobule and right medial superior frontal gyrus were found among groups(FWE correction,all P＜0.05).Compared with NC group,FC of left cingulate gyrus decreased,of right inferior frontal gyrus and right medial superior frontal gyrus increased in CI group(FWE correction,all P＜0.05).The decreased FC value of left cingulate gyrus was negatively correlated with clock drawing test score in CSVD patients(r=-0.159,P=0.049).Conclusion CSVD patients with or without CI had extensive abnormal FC of FPN,and the left cingulate gyrus was associated with patient's cognitive function.