Objective To establish the fingerprint of amino acids composition of Jinlong capsule. Methods Using 6-acetamido-4-hydroxy-2-methyl quinoline (AQC) as the derivating agent, 17 kinds of amino acids were analyzed by UPLC at the same time and the fingerprint of amino acids composition of Jinlong capsule was established. Result Totally 22 co-possessing peaks were selected as the fingerprint peaks, 17 kinds of amino acids were identified. The fingerprint similarity value of 10 batches was above 0.9. Conclusion The fingerprint is characteristic and can be used for the quality control of Jinlong capsule.

Objective To discuss medicine using regularity of TCM treatment for hypothyroidism. Methods Articles in the CNKI from Jan. 2000 to Dec. 2013 were searched. Articles about TCM treatment for hypothyroidism were screened for the establishment of database. Microsoft Excel was used to analyze the frequency of main medicine, composing principle, and combination of new medicine in the articles. Results Totally 150 cases of prescriptions about hypothyroidism were screened, which involved 178 Chinese herbal medicines. Poria in the prescriptions appears with the highest frequency;Atractylodis Macrocephalae Rhizoma and Poria have the highest frequency of medicinal pair. 14 core combinations and 7 new combinations were obtained through evolution. Conclusion Most doctors’ TCM treatment for hypothyroidism in CNKI is to replenish qi to invigorate the spleen, warm the kidney to tonify yang, and excrete water and dampness.

BACKGROUND:Exosomes play a role in all stages of wound repair,and there is currently a large body of research on exosomes in skin wound repair,which has been shown to have great potential for clinical applications. OBJECTIVE:To summarize and discuss the main mechanisms and clinical applications of exosomes in the treatment of skin wounds,in order to promote the clinical translation of exosomes. METHODS:PubMed,clinicaltrials.gov,China National Knowledge Infrastructure,Food and Drug Administration database,and Chinese Clinical Trial Register were searched from inception to March 2023.The English search terms were"exosomes,wound healing,stem cells,chronic wound,immunoregulation,inflammation,skin,therapeutic use,isolation,characterization,infections".The Chinese search terms were"exosomes,wound healing,stem cells,immunomodulation,clinical applications".A total of 79 articles were included for the summary. RESULTS AND CONCLUSION:(1)Exosomes can improve and accelerate wound healing through inflammation regulation,immune protection,angiogenesis,cell proliferation and migration,and collagen remodeling.(2)Exosomes derived from stem cells have mature preparation techniques and related mechanism research,which is currently the mainstream research direction.Non-stem cell-derived exosomes have the advantages of convenience,economy,and easy production,and can be used as a supplement for clinical applications.(3)The clinical application of exosomes is still in its infancy,but has great potential for application.Various exosome modification techniques have laid the foundation for the future development of clinically personalized services and require further research.(4)The clinical translation of exosomes faces many challenges,such as low yield,high heterogeneity,lack of unified standards for isolation,purification,and quality control,and difficulties in storage.