1.Whole-genome sequencing of SARS-CoV-2 from residual viral RNA present on positive rapid antigen test kits for genomic surveillance
Mohd Ishtiaq Anasir ; Khayri Kamel ; Nor Malizza G Adypatti ; Mohammad Syafiq Jamaluddin ; Farah Amira Ahmad ; Siti Nurhidayah Norhisham ; Muhammad Zulfazli Mohamad Sukri ; Nur Rafiqah Rosli ; Siti Norazrina Saif ; Nurul Izzati Basarudin ; Mohamad Azzam-Sayuti ; Akmal Hayat Abdul Karim ; Mahirah Kamil Puat ; Ravindran Thayan ; Rozainanee Mohd Zain
Western Pacific Surveillance and Response 2025;16(1):06-11
This report describes an approach to recover SARS-CoV-2 RNA from rapid antigen test kit (RTK-antigen) cassettes for whole-genome sequencing (WGS). RNA samples were recovered from 33 RTK-antigen cassettes for WGS, with 18 samples achieving more than 80% genome coverage. This work provides a proof-of-concept that positive RTK-antigen cassettes can be safely transported, stored and subjected to WGS, enabling swift identification of circulating variants.
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