Introduction: Previous literature suggests that good nutrition knowledge does not necessarily translate into desired eating behaviours among adolescent athletes, which may affect their sports performance. The purposes of the study were twofold, which were to examine nutrition knowledge and practice between the sexes, and to evaluate the magnitude of relationship between nutrition knowledge and practice among adolescent handball athletes in Malaysia. Methods: Three hundred and twelve male and female adolescent handball players competing in a national level competition voluntarily participated in this study. The participants were asked to complete a questionnaire which consisted of items on their demographic information and about their nutrition knowledge and eating practices. Independent t-test and Pearson’s correlation were used to compare nutrition knowledge and practice between the sexes and to describe the relationship between variables, respectively. Results: The results showed that there were no significant differences (p>0.05) observed for nutrition knowledge and practice between the sexes. Besides, the results demonstrated that nutrition knowledge had a significant but weak positive correlation with eating habit (r=0.114, p=0.003) among the athletes. Conclusion: This study showed that nutrition knowledge and practice were moderately adequate, and that knowledge does not necessarily translate into good dietary practices. Therefore, the athletes would benefit from a comprehensive and innovative nutrition education, a strategy which warrants further investigation.