1.A preliminary proteomic study of permethrin resistant and susceptible Aedes aegypti (L.)
Rosilawati, R ; Nabila, R. ; Siti Futri Farahininajua, F. ; Nazni, W.A. ; Lee, H.L.
Tropical Biomedicine 2019;36(4):855-865
The mechanism of insecticide resistance is traditionally attributed to detoxification
enzymes, target site alteration, decreased penetration of insecticides and behavioural
resistance. Other form of mechanisms, such as the role of protein(s) in resistance is unknown.
In the present study, the protein profiling of both IMR-PSS strain (permethrin-selected) and
IMR-LS strain (laboratory-susceptible) 24 hours post exposure period to permethrin was
carried out via 1D-gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/
MS). The bands which appeared in the gel of 1D-electrophoresis revealed an abundance of
proteins. The band pattern of both strains looked macroscopically alike and differed only in
band intensity. However, LC-MS/MS analysis revealed that the IMR-PSS strain produced extra
388 peptides that were not found in the IMR-LS strain, indicating that IMR-PSS strain reacted
differently from IMR-LS strain as a result of persistent exposure to permethrin. Since the
complex banding patterns of 1D-gel electrophoresis were difficult to interpret the significance
of the protein difference between IMR-PSS and IMR-LS strain, hence LC-MS/MS analysis is
ideally suited for better protein resolution and thus will allow more in-depth comparison of
the complex pattern. The findings here provide the first preliminary evidence that insecticide
resistance in mosquito induces up regulation of proteins that may be protective to mosquitoes
against insecticide and proteins could be another mechanism that contributes to development
of resistance.