Objective To observe the clinical efficacy of Peng’s eye acupuncture in treating headache.Method Sixty headache patients were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by Peng’s eye acupuncture (acupuncture at ocular points after syndrome differentiation), while the control group was by Chinese medication. The clinical efficacies were compared after a treatment course.Result The total effective rate was 93.3% in the treatment group versus 83.3% in the control group, and the difference was statistically significant (P<0.05).Conclusion Peng’s eye acupuncture is an effective method in treating headache.

Fecal specimens of 35 parasitologically confirmed cases of giardiasis, 41 acute, gastroenteritis, 23 acute baciUary dysentery, 40 normal persons and 15 jirds experimentally infected with G. lamblia were tested with conterimmunoelectrophoresis (CIE). It was found that 33(94%) out of the 35 giardiasis patients and 14(93%) out of 15 infected jirds showed positive reaction, while fecal specimens of other cases, normal persons, or normal jirds all showed negative reaction. Besides, CIE was performed in 4 giardiasis patients before and after metronidazole treatment. Prior to metronidazole, they were all CIE positive, one day post metronidazole, 3 of them were still CIE positive, and Giardia cysts or trophozoites were also present in their stools. However, from the second day onwards, all of them became CIE negative, while cysts or trophozoites also disappeared from their stools. Apparently, detecting Giardia antigen in fecal specimens with CIE of feces is not only a sensitive tool for detecting current infection, but also a, useful tool for evaluating therapeutic effects in giardiasis.

This article was aimed to analyze literatures on preventive treatment of disease in acupuncture and moxi-bustion in order to understand its status and development trend. Bibliometrics and statistics method in NoteExpress were used in the analysis of related literatures about preventive treatment of disease in acupuncture and moxibustion from 1981 to 2013, which were included in CNKI. The analysis and research were from aspects such as year distri-bution, keyword, journal distribution, author distribution, and fund distribution. The results showed that there were 537 articles in total from 1981 to 2013. The number of literatures was in an overall growing trend.“Zusanli”,“Inner Canon of Huangdi”,“sub-health”, and“clinical research”were high frequency keywords. The journal distribu-tion conformed to the Brad Ford’s law. The author distribution conformed to the Lotka’s law. The number of fund papers was also increased. It was concluded that research on preventive treatment of disease in acupuncture and moxibustion was still in the period of rapid development and had not reached maturity. The preventive treatment of disease in acupuncture and moxibustion had broad application prospects. However, the research still required to be strengthened.

Objective To explore the functional expression and temporality of MDR1 gene in bone marrow of rabbits after autologous bone marrow transplantation with MDR1 transferred bone marrow mononuclear cells. Methods The supernatant of the amphotropic virus producer cell line PA317-HaMDR1/A was collected and concentrated to cocultivate with the bone marrow mononuclear cells of the rabbits. After large dose of chemotherapy with cyclophosphamide,the transferred cells were autotransplanted into the bone marrow. The integration,transfection rate and physiological function of MDR1 gene were tested by PCR,SP immunohistochemical method and daunorubicin (DNR) extrusion test respectively. Results After autologous bone marrow transplantation had been executed for 1-4 months,the integration of MDR1 gene in genome of bone marrow mononuclear cells was detected by PCR,and the expression rates of P-gp in cells tested by SP immunohistochemical method were 9.5%,8.5%,6.0% and 3.5% respectively. The physiological function of MDR1 gene in bone marrow cells was proved by DNR extrusion test. Conclusion After the autotransplantation with bone marrow mononuclear cells transferred by MDR1 gene,the MDR1 gene can implant into the bone marrow of rabbits and has expressed functionally for 4 months,which has provided a basis for further research on chemoprotection experiment of the MDR1 gene transferred into the bone marrow cells.

Objective To evaluate the clinical application of CT virtual cystoscopy (CTVC). Me-thods Thirteen patients who had clinical indication were studied. Iodine contrast was intravenously injected after the bladder was voided. Thin-slice spiral CT scanning of the bladder area was started when the bladder became filled with contrast media. By using 3D navigator smooth software, CTVC images were obtained. The findings in all cases were compared with the cystoscopy and surgical results. Results Nine patients with CTVC were proved vesical tumor,3 patients with CTVC were proved benign prostatic hyperplasia, and they were confirmed by cystoscopy,1 patient with cystitis glandularis could not be diagnosed with CTVC.By CTVC, the diagnostic accordance rate was 92.3%,the coincidence rate was 100.0% in vesical tumor. Conclusions CTVC is highly sensitive in displaying the vesical lesions.Being a new and non-invasive technique, CTVC is very helpful in the diagnosis of introvesical diseases. It has fine value in the clinical practice.

Objective:To study the silencing gene expression level of RhoGDI2 small interfering RNA(siRNA)and the colorectal cancer cell malignant behaviors such as cell proliferation,migration,invasion.Methods:Testing RhoGDI2 expression using Westen blot analysis and Real-time reverse transcription polymerase chain reaction(RT-qPCR)in the colorectal cancer cell lines of RKO,HT29,SW620,SW480,HCT116.The siRNA of RhoGDI2 with Lipofecta mineTM2000 was transfected into target cells,as well as negative control and normal control groups.Cell counting kits(CCK-8)to detect proliferation,Wound healing assay and the Transwell plate migration and invasion was detected.The epithelial-mesenchymal transition(EMT)relevant protein E-cadherin/Vimentin expression was detected.Results:Human colon cancer cell lines RhoGDI2 expression levels decreased in the order of RKO,HT29,SW620,SW480,HCT116:siRNA inhibited RhoGDI2 expression rate of RKO cell by 70%;in silence group,negative control group and blank contro1 group,the proliferation rates were(0.683±0.013),(0.866±0.088),(0.905±0.008),P<0.05;Wound healing assay and Transwell assay suggested RhoGDI2 silencing could inhibit migration;siRNA interference of colon cancer cells downregulated Vimentin,but upregulated E-Cadherin protein.Conclusion:RhoGDI2 down-regulation could significantly inhibit the cell proliferation,migration,invasion of colon cancer cell.

Objective To construct a recombinant adenovirus encoding ?-galactosidase gene(?-gal),which may be used to treat the lactose intolerance caused by lactase deficiency,with modified AdEasy system.Methods The sequence fragment of ?-gal was cloned into the shuttle plasmid pAdTrack-CMV,and the homo-logous recombination was completated with backbone plasmid pAdEasy1 in the E.coli BJ5183 to construct the recombinant adenoviral plasmid.The adenoviruses were packaged and amplified in the HEK293 cells mediated by liposome.The viral titre was measured by limiting dilution assay and the transfection efficiency was observed by X-gal histochemical staining.Results The recombinant adenovirus with ?-gal was constructed successfully and the viral titre was(2.5~4.0)?1011EFU/ml.More than 70% HEK293 cells were transfected when the MOI was 10.Moreover,more than 65% cells were blue after X-gal histochemical staining.Conclusion Recombinant adenovirus carrying ?-gal with high titre and efficient transfection is constructed successfully by AdEasy system,which supplies valuable experimental basis for the gene therapy to the lactose intolerance caused by lactase deficiency.

[ Abstract] Ischemic brain injury is closely associated w ith oxidative stress, neuron loss, and inflammatory reaction. The thioredoxin system is an important antioxidant system in human body. It plays the important roles in the process of fighting against oxidative stress damage, inhibiting apoptosis and inflammation. In recent years, the roles of thioredoxin system in cerebral ischemia have attracted more and more attention. This article review s the roles of thioredoxin in cerebral ischemia.

Objective The study aimed to explore the reliability and validity of public health practitioners 'job satisfaction scale. Methods A questionnaire survey was conducted with professional and technical personnel in the province engaged in the work of public health of investigation;they were enrolled through the multi?stage cluster random sampling method for establishing the scale 's internal consistency reliability and structur?al validity. Results The Cronbach's alpha coefficient of the total satisfaction scale was 0.900. The Cronbach's alpha coefficients were 0.896, 0.781,and 0.799 for career development and achievement,relationship and career retention,and income and promotion latitudes,respectively. Four principal components were extracted and the results showed that the cumulative contribution rate was 69.72%and the factor analysis results were basically consistent with the theoretical structure. Conclusion The scale is thus considered to be a reliable and effective measurement tool assessing public health practitioners'job satisfaction,as it had good reliability and validity.

Objective:To explore the expression and clinical significance of RhoGDI2 in colorectal cancer. Methods:Immuno-histochemistry was used to identify RhoGDI2 expression in clinical samples of colorectal cancer tissues,para-tumorous tissues and lymph node metastasis tissues. The relationships between CRC clinical factors and survival were analyzed. Results: RhoGDI2 expression contributed positively with tumor progression and metastasis in clinical tissues. It was associated with the stage of the tumor,lymph node metastasis, remote metastasis, venous invasion and vessel invasion. Patients with higher RhoGDI2 expression had poorer overall survival. Conclusion:RhoGDI2 showed high expression in colorectal cancer and it was associated with the stage of the tumor,lymph node metastasis,remote metastasis, venous invasion and vessel invasion. Patients with higher RhoGDI2 expression had poorer overall survival.