Objective To observe the expression of S100β protein in the traumatic brain injury and investigate its relation to the severity of the TBI patients.Methods To collect 30 volunteer controls,30 patients with traumatic brain injury and 30 patients with trauma expect traumatic brain injury.according Glasgow Coma Scale(GCS),TBI patients were divided into tow groups,the minor group is GCS≥8,the severegroup is GCS<8.ELISA method was used for observing the expression of S100β protein in serum from the controls and patients.Results Within 6 hours after TBI,the concentration of S100β protein increased higher in patients of TBI than the others(P<0.05).The concentration of S100β protein increased higher in the severe group(GCS<8)than the minor group(P<0.05).The higher level of seium S100β protein,the more severe of TBI patients,the higher level of serum S100β protein.Conclusion The serums S100β protein can be a special index for the early diagnosis of TBI,the higher level of it,The more severe of patients.

ObjectivesTo investigate the effect of pediatric trauma life support (PTLS) curriculum on the improvement of rescue skill for pediatric trauma patients.MethodsPTLS course was introduced to train medical staff engaging in pediatric critical trauma care. The course included theoretical and practical part with main focus on enhancement of students’ decision on scene and rescue skill.ResultsCompared with pre-training, the students’ post-training written test scores improved signiifcant-ly, and the rates of qualiifcation and excellence were improved signiifcantly (P<0.01). All students were qualiifed for the tests of skill stations and simulation scenario. The excellence rates of the tests were as follows: ifxation and transportation 42.62%, basic and advanced airway management 81.97%, shock evaluation and management 70.49%, simulation scenario 72.13%. Both pre-training and post-training written test scores in hospital group were significantly higher than those in pre-hospital group (P<0.01).ConclusionsAfter PTLS courses, the knowledge, practice and integrated life-saving skills of students are greatly im-proved and teamwork is strengthened.