Objective To explore the inhibitive effect of BSP on Caspase dependent apoptosis of preosteoclasts RAW264.7 cells by bone sialoprotein (BSP).Methods RAW264.7 cells were treated with the inhibitors of signaling pathway molecules and/or BSP.The activities of caspase3/8/9 in RAW264.7 cells were detected with caspase3/7/8/9 kits and the interaction between Caspase-8 and integrin αVβ3 was detected by Co-IP.Results The activities of Caspase3/8/9 in BSP-treated RAW264.7 cells were decreased and the apoptosis of RAW264.7 cells were inhibited significantly.In addition,there was a positive correlation between caspase-8 activity and caspase-3 activity.There was interaction between caspase-8 and integrin αVβ3.Conclusion The anti-apoptotic effect of BSP on RAW264.7 cells is mainly through the regulation of exogenous apoptosis pathway and the apoptosis induced by caspase-8 is inhibited by the binding of BSP and αvβ3.

Objective To explore the key signaling pathways and molecules of bone sialoprotein (BSP) to promote proliferation and differentiation of preosteoclasts RAW264.7. Methods RAW264.7 cells were treated with BSP and the inhibitors of signaling pathway molecules. MTS and TRAP staining kits were used to evaluate the effects of proliferation and differentiation. The activity assay kits of Calcineurin, AKT, JNK, ERK and p38 were used to detect their activities. Results Inhibiting ERK and p38 can inhibit cell proliferation induced by BSP significantly, while inhibiting AKT, Calcineurin and PI3K can reduce the role of promoting the differentiation by BSP. With increasing treatment time of BSP, the activity of Calcineurin in RAW264.7 cells increased. Furthermore, the activity of AKT was maximum at 48 h after treated with BSP, while the activity of JNK, ERK and p38 were maximum at 24 h after treated with BSP. Conclusions BSP mainly regulates the ERK and p38 of MAPK pathway to promote RAW264.7 cell proliferation, and regulates AKT, PI3K and Calcineurin pathways to promote RAW264.7 cell differentiation.

Body temperature is an essential physiological parameter. Conducting non-contact, fast and accurate measurement of temperature is increasing important under the background of COVID-19. The study introduces an infrared temperature measurement system based on the thermopile infrared temperature sensor ZTP-135SR. Extracting original temperature date of sensor, post-amplification and filter processing have been performed to ensure accuracy of the system. In addition, the temperature data of environmental compensation which obtained by polynomial fitting is added to the system to further improve measurement accuracy.
Algorithms ; Body Temperature ; COVID-19 ; Humans ; Temperature ; Thermometers

Breathing is of great significance in the monitoring of patients with obstructive sleep apnea hypopnea syndrome, perioperative monitoring and intensive care. In this study, a respiratory monitoring and verification system based on optical capacitance product pulse wave (PPG) is designed, which can synchronously collect human PPG signals. Through algorithm processing, the characteristic parameters of PPG signal are calculated, and the respiratory signal and respiratory frequency can be extracted in real time. In order to verify the accuracy of extracting respiratory signal and respiratory rate by the algorithm, the system adds the nasal airflow respiratory signal acquisition module to synchronously collect the nasal airflow respiratory signal as the standard signal for comparison and verification. Finally, the root mean square error between the respiratory rate extracted by the algorithm from the pulse wave and the standard respiratory rate is only 1.05 times/min.
Algorithms ; Electrocardiography ; Heart Rate ; Humans ; Photoplethysmography ; Respiration ; Respiratory Rate ; Signal Processing, Computer-Assisted ; Sleep Apnea, Obstructive

This study introduces a portable multi-channel EEG signal acquisition system. The system is mainly composed of EEG electrode connector, signal conditioning circuit, EEG acquisition part, main control MCU and power supply part. The low-power EEG acquisition front-end ADS1299 and STM32 are used to form the signal acquisition and data communication part. The collected EEG signal can be transmitted to the PC for real-time display. After relevant tests, the system has small volume, low power consumption, high signal-to-noise ratio, and meets the requirements of portable wearable medical devices.
Electric Power Supplies ; Electrodes ; Electroencephalography ; Signal Processing, Computer-Assisted ; Signal-To-Noise Ratio