1.Synthesis of CCK-8 tetrapeptide fragment by enzymatic method.
Guangya XIANG ; Heiner ECKSTEIN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2003;23(3):234-241
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.
Catalysis
;
Chymotrypsin
;
Oligopeptides
;
Papain
;
Peptide Fragments
;
Sincalide
;
chemical synthesis
2.Synthesis of CCK-8 tetrapeptide fragment by enzymatic method.
Guangya XIANG ; Heiner ECKSTEIN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2003;23(3):234-5, 241
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.
Catalysis
;
Chymotrypsin
;
*Oligopeptides
;
Papain
;
Peptide Fragments
;
Sincalide/*chemical synthesis