1.Expression of IgH/CCND1 fusion gene and cell cycle associated protein in mantle cell lymphoma
Shunping CHEN ; Wenqiao WU ; Hongwu SHEN ; Zongkai ZOU ; Haiyan SU ; Siling JI ; Shaojun HONG
Chinese Journal of Clinical and Experimental Pathology 2017;33(5):511-514
Purpose To investigate the expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene in mantle cell lymphoma (MCL) and their relationship with each other.Methods The expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene were detected on the 40 cases of MCL (expreimental group) and 20 cases of reactive hyperplasia (control group) by using the combined detection of fluorescence in situ hybridization (FISH) and immunohistochemistry of EnVision two methods.40 cases of MCL were confirmend by using gene rearrangement technique and immunohistochemistry.The threshold of IgH/CCND1 fusion gene of MCL was established in the control group.Results In the experimental group,Cyclin D1 protein positive expression rate was 100%,the positive expression of CDK4 protein rate was 87.50%,p16 protein positive expression rate was 17.50%.Positive rate of IgH/CCND1 fusion gene of 100%.These cell cycle related protein and IgH/CCND1 fusion gene were negative in the control group.Conclusion In MCL,Cyclin D1-CDK4-p16 pathway is consistent with the principle of tumor cell cycle regulation.The establishment of threshold value of IgH/CCND1 fusion gene by FISH technique may provide the basis for the judgement of FISH of the IgH/CCND1 in China.
2.The establishment of high-throughput neutralization titer evaluation model for hepatitis E virus (HEV).
Fan YANG ; Zimin TANG ; Siling WANG ; Wei CAI ; Guiping WEN ; Wenfang JI ; Jingfei YU ; Ke ZHANG ; Ningshao XIA ; Zizheng ZHENG
Chinese Journal of Virology 2015;31(1):1-6
The lack of effective in vitro infection model for hepatitis E virus (HEV) has greatly hindered the quantitative analysis of neutralizing titers of anti-HEV antibodies and human sera, thus impeding further studies of HEV-stimulated antibody responses and the immunological mechanisms. In order to improve this situation, the infection of HepG2 cells that are inefficient for HEV replication was continuously monitored until the viral load reached the limit of detection on day 13, the results of which confirmed the feasibility of using this cell line to establish the infection model. Then, neutralization assays of five anti-HEV murine monoclonal antibodies and serum samples collected from four HEV vaccine recipients (collected before and after vaccination) were performed by 96 multi-channel parallel infections, nucleic acid extraction, and qPCR. The results showed that the cell model can be applied for quantitative evaluation of the neutralizing capacity of different antibodies and antiserum samples from HEV vaccine recipients. In this study, we have successfully established a high-throughput in vitro HEV replication model, which will prove to be useful for the evaluation of HEV vaccines and studies of HEV epitopes.
Animals
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Antibodies, Viral
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analysis
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immunology
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Hepatitis Antibodies
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analysis
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immunology
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Hepatitis E
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immunology
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virology
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Hepatitis E virus
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chemistry
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immunology
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physiology
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High-Throughput Screening Assays
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methods
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Humans
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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methods
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Virus Replication