Objective To evaluate the clinical effects of fiberoptic bronchoscopy and bronchoalveolar lavage in the treatment of refractory Mycoplasma pneumoniae pneumonia.Method The clinical data of 55 children with refractory Mycoplasma pneumoniae pneumonia were retrospectively analyzed during February 2015 to February 2016.Results Among those 55 children,30 cases who received the treatment of bronchoscopy and bronchoalveolar lavage were assigned to treatment group,and the other 25 cases were assigned to control group.17 children in treatment group and 13 children in control group had lung lamellar shadow.After treatment,the improvement rates were 100％ and 69.2％ in treatment group and control group,respectively.There was significant difference between two groups (P=0.026).The total effective rate in treatment group was 96.7％,which was significantly higher than that of control group (64.0％) (P=0.006).The length of hospital stay were significantly shorter and average treatment fee were significantly lower in treatment group than those in control group (P＜0.01).There were no severe adverse reactions in treatment group.Conclusion The effects of fiberoptic bronchoscopy and bronchoalveolar lavage with local application of drug were remarkable in the treatment of refractory mycoplasma pneumonia,and there were no severe adverse reactions.

Objective To explore the role of transcription factors such as nuclear factor-kappa B(NF-?B) and glucocorticoid receptor(GR) in the pathogenesis of Adriamycin(ADM)-induced nephrosis in rats and the therapeutic effects of dexamethasone(Dex) and cyclosporin(CsA) on these animals.Methods The DNA-binding abilities of NF-?B and GR in cortex of kindey were examined with electrophoretic mobility shift assay(EMSA) and isotopic radioautography on the 7th,14th,21th and 28th day after a single intravenous injection of ADM,and the therapeutic effects of Dex and CsA were estimated.The biochemistry parameters from blood and urine of rats and the urine protein excretion were also measured.Results The NF-?B DNA-binding ability was significantly increased after 7 days and achieved maximum after 28 days(P0.05).Conclusion The DNA-binding ability of NF-?B is abnormally increased and that of GR is decreased in cells from cortex of kindey in Adriamycin-induced nephrotic rats.The NF-?B DNA-binding ability can be inhibited and the urine protein excretion is decreased by the treatment of CsA.

BACKGROUND:In recent years, some studies have demonstrated that ganglioside can promote survival and differentiation of umbilical blood cord mesenchymal stem cel s in vitro. OBJECTIVE:To observe the effect of injection of human umbilical blood cord mesenchymal stem cel s and ganglioside into rat lateral ventricles on neurological functional recovery from cerebral palsy. METHODS:Total y 60 cerebral palsy neonatal rats were delivered from pregnant rats which were modes were given intraperitoneal injection of lipopolysaccharide for 2 successive days on day 17 of gestation. Then those neonatal rats were randomly divided into five groups, including model group (n=10), sham transplantation group (n=10), stem cel transplantation group (n=18), ganglioside group (n=10) and combination group (n=12). Under stereotaxic instrument, umbilical blood cord mesenchymal stem cel s or ganglioside were injected into left lateral ventricles of the rat brain, respectively, and the sham transplantation group was given the same volume of phosphate buffered saline. Two rats from the stem cel transplantation group were put to death for immunofluorescence staining at 7, 14, 21 and 28 days after transplantation, respectively, and two rats in the combination group were kil ed for immunofluorescence staining at 14 days. Besides, al rats were underwent neurologic evaluation at 28 days after transplantation. RESULTS AND CONCLUSION:The umbilical blood cord mesenchymal stem cel s could survive, migrate and differentiate, which mainly distributed in the lateral ventricle, hippocampus and cortex. At 14 days after transplantation, positive expressions of BrdU and glial fibril ary acidic protein in the combination group were significantly higher than those in the stem cel transplantation group (P<0.05). In addition, compared with the model group, the holding time significantly prolonged and foot error times significantly decreased in the latter three groups (P<0.05), as wel as in the combination group compared with the stem cel transplantation and ganglioside groups (P<0.05). These results indicate that umbilical blood cord mesenchymal stem cel s and ganglioside can both improve neurological function of rats with cerebral palsy. Given that ganglioside can promote survival and differentiation of umbilical blood cord mesenchymal stem cel s in vivo, the combined transplantation is preferred.

The sodium and water retention is one of the characteristics in nephrotic syndrome. Its mechanism remains to be determined though it has been suggested that vasopressin may play an important role on the water retention. Objective　 To explore the possible effect of antidiuretic hormone on water retention in experimental nephrotic rats. Methods　 Urine and blood osmolalities were determined by using the cryoscopic method for evaluation of clearance of solute-free water (CH2O). The activities of adenosine cyclase (AC), adenosine 3′,5′-cyclic monophosphate phosphodiesterase (cAMP-PDE) and the amount of cAMP in renal medulla of experimental nephrotic rats were measured by using radioimmunoassay. The study included 6 groups of rats. Rats in group 1 were normal controls, in group 2 were injected with DDAVP, in group 3 injected with DDAVP antagonist, in group 4 injected with adriamycin, in group 5 injected with Adriamycin + DDAVP, and in group 6 injected with adriamycin + DDAVP antagonist. Results　 The urine osmolalities were (1 772±879), (2 145±936), (1 764±498), (1 050±272), (1145±243) and (993±196) mOsm/kg H2O in rats of group 1 to group 6, respectively ( F=113.069, P=0.000 4). The plasma osmolalities were (302±9), (296±10), (308±6), (286±5), (289±6) and (290±7) mOsm/kg water, respectively (F=17.302, P=0.014 2). CH2O were (-0.8±0.4), (-1.4±0.7), (-1.1±0.6), (-1.0±0.4), (-1.0±0.6) and (-0.9±0.5) ml/h, respectively (F=0.024, P=0.884 3). AC activities were (237±109)，(329±36)，(231±10)，(251±64)，(249±26) and (252±38) pmol/mg protein* min, respectively（F=0.659, P=0.4630）. The amounts of cAMP were (652±152)，(709±84)，(567±39), (77±16), (83±21) and (84±26) pmol/mg protein(F=177.033，P= 0.000 2), respectively. The percentages of cAMP-PDE transformation were (0.19±0.09), (0.18±0.07), (0.20±0.11), (0.34±0.08), (0.35±0.05) and (0.32±0.03) %, respectively (F=11.209, P=0.029 0) in rats of group 1 to group 6. Conclusion The antidiuretic hormone may not play a role on water retention in rats with adriamycin nephropathy, which might result from the decreased amount of cAMP accompanied with the increased cAMP-PDE activity, and the un-increased AC activity stimulated by DDAVP in renal medulla of rats after ADR injection.