1.Characteristics of the peripheral blood CD45RA~+ and CD45RO~+ T lymphocyte subsets in the patients with chronic hepatitis B and its clinical significance
Shengli BI ; Sien ZHAN ; Yuguo SONG ; Jianwen HUANG
Chinese Journal of Immunology 1985;0(06):-
Objective:To characterize the CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+T lymphocyte subsets in the peripheral blood of the patients with chronic hepatitis B and to explore their relations with the disease state.Methods:The peripheral blood was collected from 104 patients with chronic hepatitis B and 30 healthy individuals,then CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+T lymphocyte subsets were detected by flow cytometry with three-color fluorescence technology.Results:To compare with the control,the percent of CD8+CD45RA+T lymphocyte in patients with mild and severe grad CHB decreased markedly,the percent of CD8+CD45RO+T lymphocyte increased markedly,CD4+,CD8+,CD4+CD45RA+ and CD4+CD45RO+T lymphocyte did not change obviously. Compared with the mild grade CHB,the percent of CD8+CD45RA+T lymphocyte in patients with severe grade CHB decreased obviously(P
2.Human-human antibody conjugate as positive control in the immunoassays for measuring specific IgM to hepatitis A virus
Min LIU ; Ruihuan XU ; Sien ZHAN ; Kuo ZHANG ; Rui ZHANG ; Jinming LI ; Lunan WANG
Chinese Journal of Laboratory Medicine 2009;32(8):944-948
ed to construct a series of positive control materials that are applicable to many other immunoassays.