AIM: To investigate the differential expression profile between nasopharyngeal carcinoma cell line CNE1 and its steady EBV-LMP1-transfected cell line CNE1-LMP1, and to explore the regulatory effect of LMP1 on oncomiRs expression in CNE1 cell line. METHODS: A microRNA array that targets 132 of the most well studied oncomiRs was used to detect the expression profile of CNE1 and CNE1-LMP1. qRT-PCR assay were used to verify the expression data detected by microarray. RESULTS: Among the restricted 132 miRNAs, 30 were detectable. Among which, 30 were expressed in CNE1-LMP1, 19 in CNE1 and 11 were specifically expressed in CNE1-LMP1. Among the 19 shared miRNAs, the expression level of 6 miRNAs (hsa-miR-19b, hsa-miR-17-3p, hsa-miR-22, hsa-miR-149, hsa-miR-150 and hsa-miR-188) elevated over two folds in CNE1-LMP1. No decrease in miRNA expression more than two folds was observed. qRT-PCR confirmed the expression difference of these six miRNAs (P<0.01). Among the 11 specifically expressed miRNAs in CNE1-LMP1, hsa-miR-122a showed the highest expression level surpassing the internal control sample. CONCLUSION: Our data suggest that LMP1 may play an important role in regulating the expression of miRNAs in tumor, which may be another important pathway employed by LMP1 in the development of nasopharyngeal carcinoma.

Objective To explore the effects of applying standards of diagnosis-related group ( DRG) nursing quality to patients with parotid pleomorphic adenoma .Methods Sixty-four patients in our department with parotid pleomorphic adenoma were randomly divided into control group ( n=32 ) and experimental group ( n=32 ) . The control group received traditional nursing care , while the experimental group received departmental standards of DRG ( parotid pleomorphic adenoma ) nursing.The nursing quality and effects were compared, as well as the satisfaction of patients .Results The related knowledge understanding rates in the experimental group and control group were 93.75% and 71.88%, respectively, which showed a significant difference (χ2 =7.540,P<0.05).And patients’ satisfaction rates in the experimental group and control group were 90.63% and 81.25%, respectively, which showed a significant difference (χ2 =13.506,P<0.05). There were significant differences in the mean of hospitalization days and hospitalization expenses (t=730.00, 883.50,respectively;P<0.05).Conclusions The new quality standards and template of DRG in patients with parotid pleomorphic adenoma can make nursing work more standardized , and can improve nursing quality effectively.The hospitalization days and expenses can be significantly reduced and the understanding of health education and patients ’ satisfaction can be improved .