Objective This paper aimed to investigate the collaborative coding of two different modes of neural signals including local field potentials and spiking activity (multi-unit activity) which recorded in medial prefrontal cortex of sprague-dawley (SD) rats in Y maze working memory (WM) task,to provide computing support for neural coding mechanism of WM.Methods 1.Experiment data was multi-channel neural signals (local field potentials (LFPs)-Spikes) recorded from prefrontal cortex of 4 SD rats during WM process and resting state,provided by the lab of Neuro-engineering,Tianjin Medical University.2.LFPs preprocessing included baseline drift removing and power-line noise eliminating.3.Physiology window width 500 ms,step 125 ms were selected and average rate per channel was calculated to turn the discrete point signal spikes to continuous signal.4.LFPs characteristic frequency band was obtained by using short time Fourier transform and signals of the characteristic frequency band were extracted by band pass filter.5.Mutual information (MI) of LFPs-spikes was computed to get the distribution of multi-channel MI values.Results 1.LFPs distribution demonstrated that the power focused at θ band (4-12 Hz)during the WM tasks.2.The average MI value of θ band LFPs and spikes (4 rats,over 10 trials) were 0.49±0.04,0.39±0.03,0.41±0.03,0.48±0.02,respectively,which were significantly larger than those in the resting state (t test,P＜0.05).Conclusions These findings indicate that θ band LFPs represents behavior correlated to WM and its synergy with spiking activity plays an important role in encoding WM task.

Human mammaglobin (hMAM) is a recently discovered breast tissue specific protein with a high sensitivity and specificity. The expression of hMAM in breast cancer and its relationship with breast cancer indicate that hMAM is a novel breast specific marker that can induce cytotoxic actions from immune system and therefore may have great potentials in breast cancer treatment.

Animals ; Antioxidants ; physiology ; Dehydroepiandrosterone ; physiology ; Lipid Metabolism ; physiology ; Male ; Oxidative Stress ; drug effects ; Rats ; Rats, Sprague-Dawley

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Capsules ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine ; Lung Neoplasms ; drug therapy ; Phytotherapy ; Small Cell Lung Carcinoma ; drug therapy

Objective:To study the clinical and pathological features of Langerhans cell histiocytosis (LCH), and to provide the reference for its diagnosis and treatment.Methods:The manifestation of one LCH patient was retrospectively analyzed.The features of the LCH patients were explored by analyzing the results of skin biopsy, radiological test and follow-up.The associated literatures were reviewed.Results:The patient presented the typical symptoms gradually,including polycystic lung,skin ulcer,diabetes insipidus,and lactation.The skin pathological findings showed the densely distributed mononuclear cell infiltration in dermis and the immunohistological staining result showed positive CD1a. The patient was follwed up for 7 years and died of heart and lung failure. Conclusion:LHC has various manifestations and should be confirmed by clinical features,pathological features and imaging examination.The adult patients with multisystem and vital organ involvement suggest the poor prognosis.

Adolescent ; Adult ; Female ; Health Status ; Humans ; Male ; Medical Staff ; Middle Aged ; Occupational Health ; Risk Factors ; Sampling Studies ; Surveys and Questionnaires ; Young Adult

AIM: To observe the influence of lentiviral vectors expressing siRNA for survivin gene knockdown in A549 cells,sequentially as tools to explore the molecule pathogenesis and new gene therapy of lung adenocarcinoma.METHODS: The lentiviral vectors,which express survivin siRNA,were constructed and transfected into A549 cell strain.The titers of the lentiviruses were determined by 293T cells.The expressions of survivin and caspase-3 were detected by Western blotting and RT-PCR.The cell cycle and cell growth of A549 cells were examined by MTT and FCM.RESULTS: The expression of survivin was suppressed effectively by siRNA targeting survivin.The expression of survivin mRNA decreased by 97%.The expression of survivin protein decreased by 94%.The rate of cell growth was decreased.The G1 phase cells were increased,whereas S phase cells were decreased.CONCLUSION: The lentivirus vectors expressing siRNA for survivin can significantly inhibit gene expression and the cell growth,and markedly induce the apoptosis.It is hopeful to be a new gene therapy of lung adenocarcinoma.

Cerebral Hemorrhage ; complications ; Child ; Humans ; Intussusception ; complications ; Male ; Purpura, Schoenlein-Henoch ; complications

Breath Tests ; methods ; Child ; Humans ; Infant ; Lung Diseases ; diagnosis ; physiopathology ; Lung Volume Measurements ; Maximal Voluntary Ventilation ; Respiratory Function Tests ; Tidal Volume