Medicine, Chinese Traditional ; methods ; trends

Coronary Disease ; diagnosis ; Humans ; Medicine, Chinese Traditional ; methods

Objective: To design a pair of PCR primers that could identify Dendrobium chrysotoxum specifically, to optimize the system of PCR detection, and to establish a method to identify D. chrysotoxum rapidly and accurately. Methods: From GenBank database, rDNA 170 ITS sequences in the plants of Dendrobium Sw. were downloaded and compared with all sequences using MEGA 5.0; The variation sites were located, and a pair of specific primers in the non-conservative district were designed. PCR amplification was performed using the specific primers with 35 DNA templates in the plants of Dendrobium Sw., D. chrysotoxum was positive. Results: D. chrysotoxum could be specifically amplificated by specific primers when the annealing temperature was raised to 58 °C, while other plants of Dendrobium Sw. were shown as negative, and the sensitivity of the primer could reach 0.69 ng/μL. Conclusion: This study designs a method that could identify D. chrysotoxum specifically. Using this specific primer could identify D. chrysotoxum rapidly and accurately from the homologous species. This method is well-performed in specificity, and it is more simple, convenient, efficient, and accurate than other methods, such as morphological and microscopical identification, chromatograph, and spectral method.

Objective To explore the bacterial flora distribution characteristics and drug resistance of pus bacterial culture in a‐cute mastitis and to analyze thechange trend of drug resistance spectrum to provide a evidence‐based basis for the rational use of an‐timicrobial agents in clinic .Methods The pus collected from 207 cases of acute mastitis was conducted the bacterial culture .The bacterial identification and antibacterial susceptibility test were performed by adopting the manual experiment combined with the DL‐96 system .Partial drug susceptibility test was performed by combining with the K‐B method .Results Among 207 specimens , 82 strains of pathogenic bacteria were detected with the detection rate of 39 .6% ,including 51 strains (62 .2% ) of staphylococcus aureus ,7 strains (8 .5% ) of pseudomonas aeruginosa ,4 strains (4 .9% ) of staphylococcus intermedius ,4 strains (4 .9% ) of staphy‐lococcus epidermis ,3 strains (3 .7% ) of acid‐producing klebsiella bacteria and each 1 strain of staphylococcus hemolyticus and other 13 kinds of bacterium .The resistance rates of staphylococcus aureus to azithromycin ,erythromycin and clarithromycin were 92 .2% ,84 .3% and 84 .3% respectively ,indicating that macrolides drugs had a higher overall drug resistance rate and were not suitable for selection and use;the resistance rates of moxifloxacin and ciprofloxacin were 3 .9% and 4 .1% respectively ,the MRSA detection rate was 27 .5% .The resistance rates of Pseudomonas aeruginosa to ticarcillin/clavulanic acid was 85 .7% ;the drug resitance rate of cefoperazone was 83 .3% ;which of gentamycin and amikacin was 71 .4% ;which of aztreonam was 14 .3% ;which of ceftazidime was 28 .6% and which of meropenem was 28 .6% .Conclusion The majority of detected bacteria in pus from the pa‐tients with acute mastitis are Staphylococcus aureus ,followed by pseudomonas aeruginosa ,which is different from that reported by other literatures ,showing the bacterial distribution has regional difference .Staphylococcus aureus has high resistance rate to macrol‐ides antibacterial drugs ,but is highly sensitive to ciprofloxacin and moxifloxacin;Pseudomonas aeruginosa has higher resistant rate to ticarcillin/clavulanic and cefoperazone ,but it is highly sensitive to aztreonam ,ceftazidime and meropenem .Empirical medication should be comprehensively considered by combining with drug resistance spectrum of Staphylococcus aureus and Pseudomonas aeruginosa ,and the sensitive drugs should be selected according to the drug susceptibility results after the antimicrobial susceptibili‐ty test for conducting the targeted medication .

This paper analyzed the characteristics of pathophysiology teaching for Mongolian students.Exploration was made in aspects of designing,implementing and evaluating multilingual pathophysiology teaching mode for Mongolian students.The problems of the mode were summarized and optimization measures were proposed.

Ceramics ; Ergonomics ; Female ; Humans ; Male ; Occupational Exposure ; Workload

Humans ; Occupational Exposure ; Risk Assessment ; Workplace

The progress in integrative medicine (IM) of coronary heart disease (CHD) in 2013 was summarized in this paper. Gratifying progress has taken place both in clinical studies and basic research of IM on CHD during 2013. We got some innovation in Chinese medical etiologies and pathogeneses of CHD. We also got some improvement in researches on Chinese medical syndromes in various fields. Many clinical studies have proved Chinese medicine and pharmacy is playing a role in preventing and improving CHD patients. In-depth basic researches on preventing and treating CHD by IM are gradually undergoing. Acting targets and mechanism are further clarified. Besides, we also pointed out the developing tendency of CHD researches and the current deficiency in CHD researches, hoping to provide reference for further studies in this field.
Coronary Disease ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Integrative Medicine

Adult ; Ergonomics ; Female ; Humans ; Male ; Middle Aged ; Multivariate Analysis ; Musculoskeletal Physiological Phenomena ; Regression Analysis ; Workplace

We measured IgG and IgM ACA levels by a modified enzyme一linked immunosorbent as-say(ELISA) in 122 patients with brain infarction and 86 healthy controls. Significant difference in positiveIgG and IgM ACAs was found between the patients with infarction(50.8%)and the control group(11.6%).The relation between positive ACAs and brain infarction was studied. The pathogenetic mecha-nism remains unclear.