Objective To explore the evaluation of contrast enhanced CT with multi -direction adjusting multi -planar reformation(MPR)on the stomach wall invasion of advanced gastric cancer.Methods 105 cases of advanced gastric cancer proved by pathology after surgery were checked by contrast enhanced CT before surgery.All the images were reconstructed by multi -direction adjusting MPR to show perfectly the lesions of gastric cancer,T stage diagnosed by CT were compared with the T stage diagnosed by pathology.Results T1,T2,T3,T4 stage diag-nosed by pathology were 9 cases,23 cases,46 cases and 27 cases respectively,the accuracy of multi -direction adjus-ting MPR in diagnosing T1,T2,T3,T4 stage were 98.1%,93.3%,86.7%,91.4% respectively.Conclusion Con-trast enhanced CT with multi -direction adjusting MPR can improve the accuracy in diagnosing the T stage of ad-vanced gastric cancer,which can be used to evaluate the staging of advanced gastric cancer.

Objective To explore the death distribution characteristics of heart disease and the strategies for its prevention and control, based on data of the death rates of different heart diseases among the residents of Henan province from 1987 to 2006. Methods The mortality rates of heart diseases during past 20 years were analyzed by age, sex, living places (urban vs. rural), and by patterns of heart disease as well with SAS9.1. Results In these past 20 years, the mortality rates of heart diseases ranged from 60.93 to 85.10 per 100 000. The annual mortality rate averaged 74.47 per 100 000, and the standardized mortality rate was 61.10 per 100 000, which equaled to 13.14% of the total death number. The death rates of heart diseases had increased significantly with age. Among the male and female of the cities or the rural, the death was mainly due to coronary heart disease. Concludes According to the death distribution characteristics of heart diseases in Henan Province, the prevention and control efforts should focus on the population over 35 years of age.

Objective To estimate the death number of children under age 5 in Linzhou city during 2004 to 2005 using the capture-recapture method, and to evaluate the integrality of data about the children death report in all death cause registry. Methods The data of children death were collected from sources of registry office, village symposium for the 3rd national death cause survey and police registries. Cases in every source were matched on the name, sex, age, date of death, cause of death and resident address. To fit for dependencies and heterogeneities between three sources, a log-linear model in stratified age and gender was used to estimate the death number of children which did not appear in three sources. Results It was reported that death number of children under age 5 was 359 and the mortality was 2.16% for combined three sources; but from the registry office, the number and mortality were 291 and 1.75%, respectively. The capture-recapture method estimated that the number of death was 398 and the mortality was 2.39%. Under-reporting rates of registry and aggregate database were 26.9% and 9.8%. Female deaths were more likely to go unreported. Under-reporting rates of registry got higher with the decrease of the age at death. Conclusion Registry office or aggregated database underestimated the death number of children under age 5. On the base of data which were collected from police, village and registry, using the capture-recapture method can adjust the death number of children.

Objective To observe the effect of daidzein on intima proliferation of rat common carotid artery injured by balloon. Methods 24 male Wistar rats were randomly assigned to 3 groups, 8 rats per group: high dose daidzein group (100 mg/kg?d), low dose daidzein group (50 mg/kg?d) and dissolvent control group. Duplicate the rat common carotid arteries injured by balloon models. Five weeks after operation, serum biochemical indicator such as TC, TG, BUN, ALT and MDA were measured using routine biochemical methods. And pathological sections of common carotid arteries were observed and the changes of vascular histomorphology were estimated by computer analysis of photomicrograms. Results Compared with control group, the intima areas was significant decreased in high dose group and low dose group (86 893?83 452 ?m2 vs 199 419?103 352 ?m2 vs 222 324?56 154 ?m2,P0.05). Conclusion Daidzein can inhibit the intima proliferation of Wistar rat common carotid arteries injured by balloon.

Background and purpose:Henan is a big agricultural province and the high mortality rate of digestive system cancer threatened the life and the health of the people.In order to make some good strategies,we investigated the mortality rate and characteristic distribution of digestive system cancer in Henan province. Methods :The mortality and age-standardized mortality rate from 1987 to 2006 in Henan province were analyzed by using the data from registration system of all causes of death from 1/10 population in Henan province. Results :The crude mortality rate of digestive system cancer from 1987 to 2006 in Henan province was 81.95/10 5,and the age-standardized mortality in Chinese standard population was 71.31/10 5,which accounted for 71.29% of all cancers.The mortality rates,age-standardized mortality in male was 101.17/10 5 and 94.81/10 5 respectively,compared to 61.58/10 5 and 49.28/10 5 respectively in female.The foremost four in rank of the digestive system cancer were gastric cancer,esophageal cancer,liver cancer and intestinal cancer.The mortality of digestive system cancer has descended in the recent twenty years.Comparing in 1987 and in 2006,the age-standardized mortality rates of gastric cancer and esophageal cancer had decreased significantly from 30.38/10 5 to 21.27/10 5 and from 34.12/10 5 to 15.70/10 5 respectively.The death rate of intestinal cancer remained constant.But age-standardized mortality rate of liver cancer had increased significantly from 12.45/10 5 in 1987 to 18.26/10 5 in 2006. Conclusions :The distribution of death rate of digestive system cancer had changed greatly,so the prevention measures have to be changed accordingly.

OBJECTIVE To investigate the effect of overexpession of 18 ku translocator protein (TSPO) on the hippocampal dentate gyrus. METHODS Lentiviral (LV) vectors containing TSPO or the lentiviral sequence were infused into the hippocampus bilateral dentate gyri (2 × 108 TU · mL-1,1 μL per side)of mice. Behavioral tests were carried out. The anxiolytic-like behavior of mice was examined by such means as the elevated plus maze test , the staircase test , light dark box test for 12, 14 and 16 d, two behavioral despair models, tail suspension test and the forced swimming test for 16 and 18 d,respec?tively. Western blotting and ELISA were used to evaluate the TSPO expression and the concentration of allopregnanolone in hippocampal tissue (3 mm in diameter around the injection site on both sides) at the end of tests. RESULTS The results of behavioral experiments showed that TSPO overexpression group deneloped anxiolytic and antidepression-like behavior. LV-TSPO significantly increased the retention time in the central area〔14 ± 4 vs (25 ± 12)s,P<0.05〕. LV-TSPO significantly increased the percentage of entry into open arms entries percentage and the percentage of time spent in open arms time without changing total entries and total time in the elevated plus-maze test〔(13±8)%vs (26±18)%, P<0.05;(6 ± 6)%vs (27 ± 6)%, P<0.05)〕. LV-TSPO significantly decreased the number of rearings without changing the number of steps in staircase test (21±7 vs 12±5,P<0.05). LV-TSPO increased entries into the light area and retention time in light-dark transition test〔(18 ± 8)% vs (26 ± 7)%, P<0.05;72 ± 36 vs (191 ± 90)s, P<0.05)〕but significantly decreased immobility time in the tail suspension test and forced swimming test〔94±33 vs (36±20)s, P<0.01;137±36 vs (90±37)s, P<0.05)〕, without excitatory or inhibitory actions on the central nervous system. At the same time, the level of TSPO expression in hippocampal tissues (3 mm in diameter around the injection site on both sides) was significantly increased, so did the concentration of allopregnanolone (P<0.05). CONCLUSION Overexpression of TSPO in the hippocamus dentate gyrus of mice can induce anxiolytic and antidepressant-like behavior, and the downstream allo?pregnanolone biosynthesis at least partially mediates the behavioral effects.

Objective To evaluate the performance of Beckman Coulter Enhanced troponin Ⅰ immunoassay system (including the limit of detection and total imprecision) and establish the 99th percentile of Enhanced troponin Ⅰ in apparently healthy Chinese people in Kunming and Wuhan.Methods Evaluated the limit of detection and total imprecision of Beckman Enhanced troponin Ⅰ according to protocols EP of Clinical Laboratory Standards Institute; chose apparently healthy people from Wuhan (average altitude of 27 m) which represents plain regions,and Kunming (average altitude of 1895 m) which represents plateau regions.760 subjects from Wuhan were selected,aging from 30 to 91,included 400 males and 360 females.A total of 192 subjects from Kunming were selected,aging from 30 and 77,60 patients,which included 60 male and 132 female cases.To calculate the 99th percentile of Enhanced troponin Ⅰ by region,age,and gender.Results The limit of detection of Beckman Enhanced troponin Ⅰ is 0.013 μg/L,the cTnI concentration is 0.025 μg/L at 10％ CV.The 99th percentile of Enhanced troponin Ⅰ of the total population in Wuhan is 0.036 μg/L,the 99th percentile of men and women are 0.038 μg/L and 0.035 μg/L respectively,the 99th percentile in the 30-69 years group and over 70 years group are 0.038 μg/L and 0.035 μg/L respectively.The 99th percentile of Enhanced troponin Ⅰ of the total population in Kunming is 0.040 μg/L.To keep the 2nd digit after decimal point for results from Wuhan and Kunming,the 99th percentile of Enhanced troponin Ⅰ of the apparently healthy Chinese in 0.04 μg/L,where the CV is 8.23％.The percentage of positive samples detected below the 99th percentile in the normal reference population in Wuhan is 94％.Conclusions The 99th percentile of Beckman Enhanced troponin Ⅰ of the Chinese apparently healthy people is 0.04 μg/L,where the total imprecision is 8.23％,and the detection performance reach the acceptable levels per the guideline.

Objective: In the present study we observed the general pattern of the adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose radiation (LDR). Methods: Kunming male mice were irradiated with the inductive dose (D1, 75 mGy) and the challenging dose (D2, 1.5 Gy). The intervals between D1 and D2 were 3, 6, 12, 24 and 60 hours. The changes of thymocyte apoptotic bodies (TAB) and cell cycle progression were measured with flow cytometry with the thymocytes cultured for 4, 20 and 44 hours, respectively, 18 hours after irradiation with D2. Results: When the intervals between D1 and D2 were 3, 6 and 12 hours, the percentages of TAB in the D1 + D2 groups in the thymocytes cultured for 4 and 20 hours were significantly lower than those in the D2 groups (P<0.05) and the percentages of G0/G1 and G2 + M phase cells decreased in varying degrees, while the percentages of S phase cells increased significantly (P<0.05 or P<0.01). Conclusion: The results mentioned above indicate that when the mice were irradiated with 75 mGy (D1, 12.5 mGy/min) 3～12 hours before 1.5 Gy (D2, 0.285 Gy/min) exposure, the adaptive response of apoptosis and cell cycle progression may be induced with the thymocytes cultured for 4 and 20 hours after whole-body irradiation with D2.

Objective To study how to improve velopharyngeal ring ligafion procedure by using ring ligator in velopharyngeal ring ligatian procedure and settle the problem that there is no standard to measure the interspace of the cavity of pharynx.Methods Select some deft palate patients who were between 3 to 17 ages.Then made five groups according to age (3 to 5,6 to 8,9 to 11,12 to 14,15 to 17).Then made five different types of ring ligators accrding to the normal size of the cavity of pharynx of different ages.Results The ring ligators were easily used and all the patients were repaired satisfied.Conclusion The application of ring ligator in velopharyngeal ring ligation procedure is simple and feasible.

OBJECTIVE To investigate the relationship between the anti-post-traumatic stress disorder(PTSD)effect of sertraline and nitric oxide in fear conditioning rats. METHODS Conditioned fear stress was established by electric shock with a cue tone,and fear extinction training was carried out by giving the rats only tone signals the next day. The rats were treated with sertraline(15 mg · kg-1) intragastrically within 1 h before the experiment for 8 d. Freezing time was tested at the 1st,4th and 7th day after the extinction training in rats. The NO contents were detected by Griess method and the nNOS and iNOS level on amygdala was detected by Western blotting. RESULTS The behavior tests showed that compared with normal control group ,the freezing time was significantly increased in extinction control group and extinction training group(P<0.01),indicating that the conditioned fear model of rats was successfully established. At the 1st and 4th day after conditioned fear extinction training in the rats,freezing time in sertraline(15 mg·kg-1)group was decreased compared with extinction training group (P<0.05). At the 7th day,the freezing time was significantly decreased(P<0.01),indicating that ser?traline reversed the fear response. At the same time,the contents of NO,nNOS and iNOS on amygdala of rats in sertraline group were lower than that in extinction training group(P<0.01). CONCLUSION Sertraline can promote extinction of conditioned fear memory,suggesting that sertraline has anti-PTSD effects on the model of fear condition in rats. The underlying mechanisms may be connected with NO.