Objective To study the neuroprotective effect of apigenin on microglia which was exposed to oxygen glucose deprivation and reperfusion (OGD/R), which was characterized by its influence on IL-1? and TNF-? expression. Methods Primary microglial cultures were prepared from newborn rat brain. The purity of isolated cells were identified by GSA-IB4. The cells were randomized into 5 groups:normal group, DMSO group and apigenin-treated groups (10, 25, 50 ?mol/L). The cells of DMSO group and apigenin-treated group were exposed to 8 h of OGD and 24 h of reperfusion in the presence or absence of apigenin at a range of concentrations. Culture supernatants were collected and IL-1? and TNF-? were detected by ELISA assay. Results The expression of IL-1? and TNF-? were significantly higher in DMSO group (P

The study on syndrome of TCM is a key topic on basic research of TCM. Among the whole system of TCM, the syndrome of TCM is in the core position. It is the bridge which connecting the clinic with the basic theories. This paper has carried on the discussion from the study of syndrome standardization and essence of syndromes. We reviewed and analyzed the present situation of syndrome, and explored the development and tendency of the research on syndrome. The following suggestions have been put forward:extracting the syndrome elements is the precondition and the comparison of diseases and their relevant syndromes is the main idea to establish the standardization of criterion of syndromes in clinical diagnosis. studying the essence of syndrome on the basis of system biology by methods of proteomics and metabolism to explore the scientifi c connotation of TCM syndrome.

Objective The differentially expressed genes in the progress of cellular senescence were studied. Methods Suppression subtractive hybridization was used to young and senescent human diploid fibroblast 2BS cells, then screening and analyzing the subtractive libraries. Results Two kinds of subtraction libraries were obtained, respectively representing the differential genes with higher expression in young cells or senescent cells. Screened by dot blot, thirty of genes with differential ratio larger than 2 were sequenced, showing changes in much of cell functions. Some of them also had expression change between blood samples from newborn or old people. Conclusions The differential expression of RBM4?FBXO7?TOM1 is reported during 2BS cell senescence for the first time. There are the similarities during the senescence of culture cell and organism.

Objective To do the environmental impact assessment for the water supply project of Mopan Mountain Reservoir in Harbin. Methods To collect the related data and to do the field-investigation and inspection in epidemiology, animals and insects, environmental quality sanitization and water quality, etc in the area of Mopan Mountain Reservoir and the surroundings. Results The main infectious disease in the development area of Mopan Mountain Reservoir was at the lower prevalence level and there was no fatal infectious disease such as plague , cholera in the recent ten years. Besides, there was no grievous public health emergency. There were no factories and collieries emitted poisonous chemical material in the catchment area. The results of water quality investigation showed that all the indexes were up to the related standards. Conclusion Monpan Mountain Reservoir project is not ease to cause the infectious diseases spread by water among the people. The water quality can meet the request for water source. The reservoir will not influence the ecological environment when the latent environment pollution factors are under control.

Objective To study the effects of different doses of insulin in diabetic ketoacidosisin children.Methods 44 patients with diabetic ketoacidosis in children who were treated with insulin in our hospital were selected as research object,and they were divided into group A(low dose group)22 cases and group B(high dose group)22 cases, and the blood glucose, blood calcium, serum potassium, acidosis corrected time and the levels of serum IL-6,IL-12,IL-18 and TNF-α and incidence of hypoglycemia of the two groups were compared and studied. Results The blood glucose, blood calcium,serum potassium, acidosis corrected time and the levels of serum IL-6, IL-12, IL-18 and TNF-α of the two groups had no differences, there were no significant difference(all P ＞ 0. 05), but the incidence of hypoglycemia of the group B was lower than that of group A,there was significant difference(P ＜0. 05). Conclusion The effects of low dose of insulin in diabetic ketoacidosis in children was better, blood glucose placidity decreases, and was worthy of further studying and popularization and application.

Objective:To seek differentially expressed plasma proteins of unstable angina blood-stasis syndrome.Methods:2-dimensional di rence gel electrophoresis and mass spectrometry were used to screen the di erentially expressed plasma proteins among unstable angina blood-stasis syndrome and healthy volunteers.Results:Compared with healthy people,the express of Clusterin,ApoA-I of unstable angina blood-stasis syndrome patient decreased while the expression of Fibrinogen ? chain,Vitamin D-Binding Protein,Haptoglobin ? chain,Haptoglobin ?2 chain increased.Conclusion:Fibrinogen ? chain,Fibrinogen ? chain,Haptoglobin ? chain,Haptoglobin ?2 chain,Clusterin,ApoA-I may be correlative with blood-stasis syndrome.

Objective To investigate the effects of edaranvone on lung injury induced by myocardial ischemia-reperfusion (I/R) in rats. Methods Twenty-four male Wistar rats weighing 250-300 g were randomly assigned to one of 4 groups ( n = 6 each): group Ⅰ sham operation (group S); group Ⅱ myocardial I/R and group Ⅲ and Ⅳ different doses of edaravone ( group E1, E2 ). The animals were anesthetized, intubated and mechanically ventilated. In group Ⅱ-Ⅳ myocardial I/R was induced by occlusion of left anterior descending coronary artery for 45 min followed by 3 h reperfusion. In group Ⅲ and Ⅳ edavarone 3 and 10 mg/kg was administered via right femoral vein at 1 min before reperfusion respectively. The animals were sacrificed by exsanguination at the end of 3 h reperfusion. Blood was collected for determination of serum CK-MB activity and total protein content. The left lung was lavaged and the broncho-alveolar lavage fluid (BALF) was colleted for determination of protein content. Pulmonary permeability index (PPI) was calculated. The lung tissue was obtained for determination of BD-2 mRNA and protein and TNF-α expression. Results The serum CK-MB activity, PPI,BD-2 mRNA and protein and TNF-α expression were significantly higher in group I/R, E1 and E2 than in group S,but significantly lower in group E1 and E2 than in group I/R and in group E2 than in group E1. Conclusion Edaravone can reduce myocardial I/R-induced lung injury by scavenging oxygen free radicals and inhibiting inflammatory response of lung tissues in rats.

Objective To establish axenic cultivation of Pneumocystis carinii (P.c). Methods The organisms of P.c were isolated from the bronchoalveolar lavage fluid (BALF) of the rats with Pneumocystis carinii pneumonia (PCP) and cultured in a medium which was based on IMDM (GIBCO) supplemented with S-adenosyl-L-methionine, putrescine, N-acetyl glucosamine, putrescine, L-cysteine and L-glutamine, and newborn calf serum. The organisms cultured in the system were identified by observing the morphology of cysts in smears stained with Gomori's methenamine silver nitrate stain (GMS). Ultrastructure of the cysts/trophozoites was examined by transmission electron microscopy. The sequences of mitochondria] large ribosomal DNA subunit of the cutured organisms were compared with the Pneumocysti carinii f.sp. ratti variant isolate (GenBank No U20173) and Pneumocystis carinii f.sp.hominis (GenBank No M58605). Results Five isolates of P.carinii received from BALF of 8 rats with PCP were cultured axenically and continuously in the system. The cultured organisms could be stored in frozen condition and used to reinitiate culture, and were amplified by 19-22 times within 72 h. The morphology, ultrastructure and gene sequencing of the cultured organisms confirmed that the isolated organisms were P.carinii. Conclusion Five continuously and axenicly cultured isolates of P.carinii have been received.

Objective To study the hemoprotective effects of a rotary magnetic field (RMF) to radiation-injured mice. Methods 132 male BALB/c mice were randomly divided into four groups: a normal group (N), a magnetic treatment group (M), an irradiation group(R) and an irradiation combining magnetic treatment group (R + M). Mice in the N group received no treatment. Mice in the R and R + M groups received total body irradiation with 6.0 Gy 60Co γ/rays. Mice in the M and R + M groups were treated with a RMF for one and half an hour at a time, twice a day, totally for 30 days. The survival rate was observed for 30 days. On days 0, 5, 9, 15, 21, 30, the subjects' peripheral blood cells were counted. On day 9, 23 and 30, the number of bone marrow nucleated cells (BMNCs), colony forming unit-spleen (CFU-S), spleen-body ratio, the cell cycle and apoptosis of bone marrow cells were measured. The pathological sectioning of the femur was performed and the expression level of bone morphogenetic proteins (BMP2/4) in the bone marrow was evaluated. Results ①No mice died in the N and M group. The RMF treatment increased the survival rate and survival days among the irradiated mice (P < 0.01). ②The RMF treatment increased the number of blood cells in their peripheral blood of the R + M group. ③The number of BMNCs, CFU-S and the proportation of G2 + M stage in the R + M were markedly higher than that of the R group, but the proportation of the apoptosis was lower than that of the R group on the 9th day (P < 0.05). Furthermore, the spleen index in the R + M group was also higher than that of the R group on the 23rd day (P < 0.05). ④RMF could improve the expression level of BMP2/4 in the radiation-injued mice. Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation and it accelerated the recovery of hematopeiesis and the hematopoietic microenvironment in mouse bone marrow.

AIM: To study the alteration of expression of iNOS mRNA and ecNOS mRNA in peripheral leukocytes of Wistar rats fed with fructose. METHODS: Wistar rats were randomly divided into the control group ( n =10) and the fructose feeding group( n =10). The fructose feeding group drank 12% fructose water for 6 months. The blood glucose, blood insulin, and the expression of iNOS mRNA and ecNOS mRNA in peripheral leukocytes of rats were determined. RESULTS: The levels of blood insulin ( P