OBJECTIVE:To evaluate the status quo of the application of Chinese traditional medicines andthe problemslie in the development of the application of Chinese traditional medicine.METHODS:The Chinese traditional medicines used in outpatients from Apr.2006to Apr.2007in our patient were analyzed statistically in respect of the consumption sum,con-sumption quantity and drug category.RESULTS:Leading the list in terms of consumption quantity in the outpatient depart-ment were heat-clearing drugs,detoxicating drugs,dampness-drying drugs and haemostatics.The utilization of Chinese traditional medicines in outpatients was standard on the whole,but there were still some irrational phenomena.CONCLU-SION:To enhance the curative efficacy of Chinese traditional medicines and standardize their use,the co-effort of both hospital staff and the society is much needed.

This paper reported activation of mice peritoneal macrophages by Sheng Ji Ointment (a Chinese traditional ointment for external application) were markedly inhibited from migrating out of capillary tubes by Sheng Ji Ointment. The source of Elephantine hide(象皮)is in short supply. Whether we Can use Cattlehide (牛皮)instead of the Elephatine hide for the Sheng Ji Ointment,the migrating inhibited effects of both are similar. The experimental results may throw some light on their clinical application.

AIM: To investingate the potential role of apoptosis in the development of acute lung injury(ALI) following limbs ischemia-reperfusion(LIR) in rats and the effects of raurine. METHODS: The model of LIB injury in rats was made. By using TUNEL, electrophoresis, reverse-transcription polymerase chain reaction(RT-PCR) and immunohistochemistry techniques, pulmonary apoptosis, Fas/FasL expressions and Ca~(2+), reactive oxygen species (ROS),the ratio of DNA double chain in tissue were detected in different groups.RESULTS: The rapid appearance of apoptosis in alveolar epithelial cells and pulmonary vascular endothelial cells were observed after LIR in rats. The ratio of DNA double chain decreased and tissue Ca~(2+) increased. The expression of Fas/FasL mRNA and protein was up-regulated in lung tissue of rats after LIR, which was related to the elevation of alveolar epithelial cells and pulmonary vascular endothelial cell apoptosis. Taurine decreased alveolar epithelial cell and pulmonary vascular endothelial cell apoptosis not by down-regulating expression of Fas/FasL system. CONCLUSIONS: These results suggest that the excessive apoptosis and Fas/FasL system expression may play a role in the pathogensis of ALI following LIB in rats, and taurine decreases alveolar epithelial cell and pulmonary vascular endothelial cell apoptosis.

Objective To evaluate the clinical value of bedside echocardiography in full term infants in NICU.Methods The bedside echocardiography was performed for 313 full term infants,and then they were ana-lyzed.Results The main clinical characteristics of totally 313 cases of neonates were tachypnea,heart murmur and cyanosis.Patent foramen ovale and patent ductus arteriosus were common in the no cyanotic blood shunt,no cyanotic congenital heart disease(CHD)was mainly made of atrial septum defect(31 cases),followed by ventricular septal defect (20 cases).Cyanotic CHD was made of pulmonary atresia(6 cases),complete transposition of great artery (5 cases),tetralogy of fallot(5 cases),total anomalous pulmonary venous drainage(5 cases).Tricuspid regurgitation was common in abnormal blood flow,moderate to severe pulmonary hypertension was common for full term infants. Conclusion There were many abnormal echocardiography examination results,and atypical noise was common in neonatal CHD,routine examination of bedside echocardiography for full term infants in NICU was important for early detection of CHD and may help treatment timely to improve the prognosis.

Objective To observe the changes of telomere length and MMPs level in human fibroblasts induced by UVB, and to explore their roles on skin photoaging .Methods Human skin fibroblasts were extracted and cul-tured.The 5th fibroblasts were irradiated by UVB .The morphology of fibroblasts were microscoped , and the length of telomere and the mRNA expression of COL1a1 and hTERT were detected by RT-qPCR.The expression of MMP-3 and MMP-1 were detected by Western blot .Results The fibroblasts gradually became round , wrinkled and disorderly arranged after 30 mJ/cm2 UVB irradiation for 24 h.The mRNA level of COL1a1 and hTERT and the expression of MMP-3 and MMP-1 were significantly increased after UVB irradiation compared with control , and the length of telomere was shortened .Conclusions UVB may frigger the early process of photoaging by the morphologi-cal changes of human skin fibroblasts and increasing the expression of MMP-3 and MMP-1 .

Objective: To investigate the expression of MDR1 and GFP in the human hematopoietic cells mediated by adeno-associated virus. Methods: The GFP gene was transferred into the human hematopoietic cells by AAV vectors and created strong visible fluorescence by purely molecular biological means. Using adeno-associated virus vectors, we have transferred human mdr-1 gene into human hematopoietic cells and investigated the drug resistence of human hematopoietic cells modified with mdr-1 gene. PCR analysis confirmed that mdrl cDNA had been successfully transferred into the human hematopoietic cells. An assay of MTT proved that the human hematopoietic cells modified by mdrl gene had resistance to colchicine. Results: It was about 30% of the hematopoietic cells that expressed the green fluorescent proteins. The resistance of hematopoietic cells was increased parently when the cells were infected by the crude virus stocks. Conclusion: It is conducted that the AAV vector could successfully transfer the foreign gene into the human hematopoietic cells. The cells modified with mdrl gene have increased the resistance to drugs.

Objective To quantify the temporal changes in the volume of blood flow to the skin of the forearm and lower leg during static maximum inspiratory breath-holding of different durations.Methods Blood flow to the skin of the forearm and lower leg were continuously measured with laser Doppler flowmetry in 12 healthy subjects.They were randomly selected to hold their breath for 10,20 or 40 seconds,or as long as possible.The volume of skin blood flow,the onset latency and the recovery latency were measured before,during and after the breath holding.Results Blood flow decreased significantly during each breath-hold with any durations.The magnitude of the decrease and its latencies were similar with all the durations.The average volume of skin blood flow and it's minimum value during each breath-hold were significantly lower in the forearms than in the lower legs.However,the average onset latency (4.41 ±0.44 s) and the average recovery latency (5.95 ±0.59 s) in the forearms were significantly shorter than in the lower legs (4.83 ± 0.70 s for onset and 7.33 ± 0.91 s for recovery).Conclusion The volume of skin blood flow decreases during a static maximum inspiratory breath-hold,and the magnitude of the increase is not related to the duration of the breath-hold.The generalized increase in skin sympathetic vasoconstrictor activity during a static breath-hold is greater in the forearm than in the lower leg.

Hypoxia-ischemia induces an inflammatory and immune response in the nervous system that may be important for development of brain injury. Recent data implicate that cytokines, chemokines, adhesion molecules，are involved in the recruitment of inflammatory-immune cells. A cytokines which promote emigration of leukocytes from the vascular lumen into the injured brain tissue are produced at the site of incipient cerebral infarction. The expression of mRNA for alpha- and beta-chemokines preceded the appearance of immune cells suggesting that these molecules may have a role in the inflammatory response to insults. The humoral and cell-medated immunity under the conditions of focal brain ischemia was discussed. These results together indicate that cell-cell interaction by adhesion molecules and cytokines is an important component in the pathogenesis of ischemic cerebral diseases, especially at the acute phase.Recently accumulated data show that depleting the amount of circulating leukocytes or administering anti-inflammatory chemicals such as cytokine blocking agents, anti-adhesion molecule antibodies, and immunosuppressants effectively minimize the size of ischemia induced cerebral infarction.

The conditions of Se accumulating Agaricus blazei in submerged culture with shake flask were studied in this paper, experimental results showed that the composition of the cultural medium was as follow: corn starch 30g/L,glucose 20g/L,(NH 4) 2SO 4 2g/L, yeast extract 3g/L, KH 2PO 4 2g/L, MgSO 4?7H 2O 1g/L and VB 1 100mg/L,that strains domesticated in slant with 1mg/L Se, 250mL conical bottle contained with 80mL liquid cultural medium, pH 7 , 80r/min and 12 days of cultural period were the optimum cultural conditions,and that on such conditions , dry weight of the mycelium reached 18 6g/L,Se accumulated in the mycelium reached 55 32?g/g and Se accumulation rate reached 5 14%

AIM To determine whether microinjection of ? amyloid peptide fragment 25～35 (A?25～35 ) into rat hippocampus induces the learning and memory dysfunction.METHODS Microinjection of A? 25～35 into hippocampus induced the rat learning and memory dysfunction. Step down test, Shuttle box test, and Morris water maze test were used to evaluate rat learning and memory function;The sections were stained with haematoxylin eosin (HE),the amyloid deposits were detected using Congo Red and silver.RESULTS The latency of A? 25～35 treated rats shortened,the stimulating time prolonged,and the numbers of errors increased in the step down test;Shuttle box test showed that A? 25～35 caused the latency and numbers of active escape reduction and the numbers of passive avoidance increase; Also in morris water maze, A?25 35 induced an impairment in rat spatial resolution; In A? 25～35 treated group, a significant decrease in the numbers of neurons in cortex and hippocampus,a massive glial reaction and neurophilic phenomenon were detected by HE staining; the positive vascular amyloidosis by Congo red and fibrils by Ag staining were also observed.CONCLUSION Microinjection of A? 25～35 into rat hippocampus may induce the learning and memory dysfunction and pathological changes which were similar to that found in Alzheimers disease (AD) brain.