Objective To investigate the influence of different diluents(physiologic saline, dis-tilled water and human inactivated serum) on measurement of 15 items of biochemical parameters.Methods Fifteen items of biochemical parameters [alanine transaminase(ALT), aspartic transaminase (AST), alkaline phosphatase( ALP), gamma glutamyl transpeptidase ( GGT), creatine kinase ( CK),lactate dehydrogenase(LD), hydroxybutyrate dehydrogenase(HBDH), total bilirubin(TBIL), direct bilirubin(DBIL), total bile acid(TBA), ereatinine(Cr), uric acid(UA), eholesterol(CHO), glucose (GLU), and blood urea nitrogen(BUN)] were chosen. For each parameter, 45 serum samples with different eoncentrations of the parameter were collected. After diluted with different diluents(physio-logic saline, distilled water or human inactivated serum), the serum samples were detected by applying the fully automated biochemical analyzer. The mean value was calculated and statistical analysis was performed. Results There were some differences of detection results when the specimens were diluted with different diluents. ALT, AST, GGT, DBIL, and HBDH serum samples could be diluted by 10 times with physiologic saline, distilled water or human inactivated serums ALP and TBA serum sam-ples could only be diluted with inactivated serum, otherwise its result would be lower; GLU, TBIL samples could be diluted with distilled water and inactivated serums for BUN, CR, UA, CK, LDH,and CHO samples, physiologic saline or human inactivated serum might be optimal; if distilled water was chosen, the results of other parameters tented to decline except UA. It was BUN was improper to dilute the BUN samples with distilled water. In addition, there was no significant difference between the items diluted by 5 times and 10 times with physiologic saline. All the 15 items could be diluted with inactivated serum. Conclusion The inactivated serum should be the first choice of diluents to e-nure the accurate results of biochemical parameters. If the prepared inactivated serum is absent, we may choose other diluents according to the above-mentioned results.

ObjectiveTo study the relationship of the parental rearing behaviors between the psychological status and post-traumatic stress disorder in the injured deliberately.Methods 161 injured people were treated in five hospitals of the Urumqi and evaluated the self-reporting questionnaire-20 (SRQ20),7-items screening scale for PTSD( PTSD7 ),impact of event scale (IES) and symptom checklist 90 (SCL-90) in the third and the fourth week injured,including 113 injured people aged at 14 ～50 years old completed the Egna Minnen av Barndoms Uppfostran (EMBU).Two months after the injury,113 injured people were interviewed,including 106 injured people diagnosed by Structured Clinical Interview for DSM-Ⅳ-TR Axis Ⅰ Disorders/Patients (SCID-I/P) and 7 injured people were lost.ResultsFather's preferences correlated with the total score of IES ( r =- 0.234,P ＜ 0.05 ) and avoid factor( r=-0.309,P＜ 0.01 ) positively,Mother's emotional warmth and understand correlated with the flashback factor of IES ( r =0.194,P ＜ 0.05 ) negatively.The injured people were divided into three groups by diagnosis:the illness-free group,the PTSD group,and other group.Parental preferences correlated with Mental symptoms negatively.Father's punish severely,interference too much,refuse or deny and Mother's interference too much,over protection and punish severely correlated mental symptoms positively.The six subscales of father's rearing behaviors and the five subscales of mother's rearing behaviors had no significant difference.ConclusionsParental preferences in childhood can internalizes inside support to protect the psychological trauma in future.Father's punish severely,interference too much,refuse or deny and mother's interference too much,over protection and punish severely can damage mental health and aggravate symptoms after traumatic event.But the influence of parental rearing behaviors is limited to decide whether the injured people suffering from PTSD or other mental disorders.

AIM:To explore the correlation between trimethylamine-N-oxide(TMAO)and type 2 diabetic kidney disease(DKD),and to provide new ideas for the early clinical diagnosis of DKD.METHODS:A total 246 patients with type 2 diabetes mellitus(T2DM)ad-mitted to the Department of Endocrinology of the First Hospital of Lanzhou University from January 1,2020 to May 31,2020 were divided into diabetic kidney disease group(DKD group)and simple diabetes mellitus group(NDKD group).According to urinary albumin/creatinine ratio(UACR),the patients were divided into A1,A2 and A3 subgroups.According to the estimated glomerular filtration rate(eGFR),the patients were divided into G1,G2,G3 and G4-5 sub-groups.According to the Kidney Disease:Improving Global Outcomes(KDIGO)guidelines,the risk of progression of DKD was assessed(low,medium,high or very high risk).General clinical data and laboratory indicators were collected.TMAO level was measured by euzymelinked immunosorbent assay.SPSS 25.0 software was used for statistical analysis.RESULTS:In T2DM patients,TMAO level was positively correlated with UACR(r=0.515,P<0.01)and negatively correlated with eGFR(r=-0.409,P<0.01).TMAO is an indepen-dent risk factor for the onset and progression of DKD.In diagnostic model,the AUROC was 0.745 with op-timal cut-off value was 5.37μmol/L.CONCLUSION:TMAO is closely related to the occurrence and de-velopment of DKD,and it has certain clinical predictive value for DKD.Therefore,TMAO may become a po-tential target for the early diagnosis and treatment of DKD.