Objective To investigate the serotype and drug resistance of pathogenic bacterium in bacillary dysentery in our region, in order to provide evidence for prevention and control of bacillary dysentery. Methods 1 617 cases of stoli samples were collected from outpatients and inpatients with diarrhea. Bacterium isolation, cultivation and biochemical identification, serotyping and drug sensitive test were carried out. Results 165 strains of shigella were isolated, among which 88 strains were S. flexneri, 40 strains were S. sonnei, 21 strains were S. boydii and 16 strains were S. dysenteriae. 13 se-rotypes and subtypes were detected, serotype 2a of S. flexneri mostly. The total susceptibility rate of snigella was highest to nalidixic acid (more than 90%), followed by that to ampicillin, rifamoin, tet-racycline, trimoxazole (70. 0%-87.5%). The susceptibility rate to norfloxacin was 20. 5%-31.3%, and lower susceptibility rate (2.5%-6. 8%) was found to eefotaxime, gentamicin and eiprofloxacin. Conclusion The main serotype of bacillary dysentery was serotype 2a of S. flexneri in our region, then S. sonnei. The susceptibility of snigella to antibiotics is increasing, so drug susceptibility test should be performed periodically.

OBJECTIVE To investigate the distribution and resistance of pathogens in lower-respiratory tract infection in our area,and help doctors to use antibiotic reasonably.METHODS According to National Guide to Clinical Laboratory Procedures,API identification system or K-B method,the pathogens of 1326 cases sputum specimens were isolated,identified and tested in drug sensitive test.RESULTS A total of 982 strains of pathogens that included 540(20.0%) G-bacilli,196(20.0%) G+ cocci and 246(25.1%) fungi were isolated from 1326 sputum specimens and the detection rate was 74.1%.From them 176 cases which occupied 13.3% were with mixed infection.The drug resistance of G-bacilli to imipenem was the lowest,then was to cefoperazone and amikacin,but the resistance of ampicillin was the highest.From 234 strains of Klebsiella had 89 strains of ESBLs-positive;in 50 strains of Escherichia coli,the strains of ESBLs-positive were 20(40.0%).The resistance rate of Staphylococcus aureus and S.epidermidis to penicillin was 97.5% and 97.2%,respectively,both of them were sensitive to vancomycin,and less sensitive to ciprofloxacin.Fifty seven strains of MRSA were detected from 90 strains of S.aureus(63.3%);and 42 strains of MRSE were detected(56.0%).CONCLUSIONS G-bacilli are the main pathogens in lower respiratory tract infection and the infection caused by fungi is more than that caused by G+ cocci,so we must pay attention also to mixed infection which occupies certain ratio.

Objective To explore clinical significance of C-reactive protein( CRP) and procalcitonin ( PCT) in chronic obstructive pulmonary disease( COPD) with acute exacerbation.Methods Included 120 COPD patients with acute exacerbations,and at the same time,according to the random number table method,120 COPD patients with stable were extracted, all respondents were given corresponding symptomatic support treatment, observation before treatment,after treatment,the 1d,7d,14d patients serum levels of CRP was associated with change PCT level. Results Compared with before treatment, after treatment, the serum levels of CRP of COPD patients with acute exacerbation of at 1d,7d,14d was associated with a decline in PCT level, different time points more statistically significant difference( all P<0.05);Compared with patients with stable,before treatment,after treatment,the 1d,7d serum levels of CRP[(12.32 ±6.59)mg/L,(9.05 ±3.31)mg/L,(7.31 ±2.31)mg/L,(4.45 ±1.54)mg/L]was associated with a high level of PCT[(0.34 ±0.05)μg/L,(0.26 ±0.04)μg/L,(0.18 ±0.04)μg/L,(0.10 ± 0.03)μg/L]were obvious,more statistically significant difference(all P<0.05);But after treatment,serum CRP was associated with differences in the level was no statistical comparison PCT significance ( P >0.05 ) .Conclusion COPD patients with acute exacerbation of serum CRP and early PCT significantly higher level,its level after treatment dropped significantly,joint observation CRP and serum PCT level can reflect COPD acute exacerbation issue of the inflammatory reaction.

Ginseng is a typical adaptogen which has resistance to various stresses. This effect is related to the hypothalamic-pituitary-adrenal (HPA) axis. As the main active ingredients, saponin has the similar structure to steroids. The regulation characteristics of ginseng saponin on the HPA axis are narrated from the aspects of total saponin and saponin monomers in this paper after the introduction of adaptation definition and HPA axis regulation mechanisms. Pharmacological effects of ginseng saponin and the regulation effect of HPA axis are summarized finally.

AIM: To establish the method of determining cinobufagin and resibufogenin in Xinkening Capsules(Venenum Bufonis,etc.). METHODS: HPLC was applied to determining cinobufagin and resibufogenin.The determination was carried out using an ODS column with a solvent system: methanol-water(50∶40).The flow rate was 1.0 mL/min,detection wavelength was at 296 nm. RESULTS: The linear ranges of cinobufagin and resibufogenin were 3.5 ?g-0.28 ?g and 3.75 ?g-0.30 ?g,respectively.The average recoveries of cinobufagin and resibufogenin were 99.86%(RSD=1.8%,n=5),and 99.84%(RSD=1.2%,n=5),respectively. CONCLUSION: This method is simple,accurate with strong specificity and can be used to control the quality of Xinkening Capsules.

Objective To study the expression of vascular endothelial growth factor(VEGF) mRNA in neonatal rats with HIBD and to explore the function of VEGF in HIBD. Methods Fourty-eight Wistar rats of 7-day-old were assigned randomly to the control or HIBD groups. The left common carotid artery of rats in HIBD group were isolated and ligated, then exposed to hypoxic environment (8%O 2) for two hours. In control group the rat left common carotid artery were isolated, but not ligated and not exposed to hypoxic environment. Animals were killed in 3 h, 12 h, 24 h and 3 d after HIBD. The expression of VEGF mRNA in hippocampus were examined by RT-PCR.Results VEGF164 was the most prominent transcript of VEGF mRNA splice variants. The expression of VEGF mRNA was present in control group, but there were no significant differences between time points ( F =0.176, P =0.911). Levels of VEGF mRNA in HIBD group were higher than that in the control group at all time points( P

Objective:To compare the efficacy and safety of mirtazapine and mianserine in the treatment of senile depression. Methods:68 senile depressive patients were randomly divided into two groups: 35 in mirtazapine group, 33 in mianserine group. The period of observation was 8 weeks, Efficacy and safety were assessed with HAMD and TESS scales at baseline, week 1, 2, 4, 6 and 8 after treatment. Results: After 8 weeks of treatment, the response rate and significant response rate were respectively 91.4% and 74.3% in mirtazapine group, 87.9% and 72.7% in mianserine group, the difference between groups were not statistically significant. The reduction of HAMD scores after treatment in both group were statistically significant (P 0.05). The common side effectives of mirtazapine were drowsiness, dizziness, increased appetite and weight gain; The common side reactions of mianserine were dizziness, nausea, drowsiness. All the side effects were mild. Conclusions:Mirtazapine and mianserine are equally effective and safe in treating senile depression.

Objective To screen and identify the serum biomarker of anovulatory dysfunctional uterine bleeding (ADUB) , to determine the expression of biomarker protein in menses of ADUB pa-tients, and to investigate the relation between ADUB and the biomarker proteins. Methods Subjects included 128 ADUB patients and 93 age-matched controls( normal women ). Their serum and super-natant of mense were collected and stored for use at -80℃. The differential proteins in the serum of the 2 groups were detected by CM 10 and analyzed by Biomarker WizardTM3.2 software. Then, the differential proteins were identified by Trieine-SDS-PAGE gel separation, spectrometry identifica-tion, and immunoprecipitation. The expression of the protein identified above in the menses was test-ed by ELISA, RT-PCR, and Western blotting. SPSS 14.1 was applied for statistical analysis and chart drawing. Results Five differential protein peaks were screened and their peak values were 11.80, 13.59, 13.79, 13.85, and 14.20 km/z, respectively. The intensity of protein peak ( 11.80 km/z ) which was identified as serum amyploid protein A ( SAA ) of ADUB was significantly higher than that of the controls (P<0.05). While the intensity of protein peak (13.59 km/z) which was identified as vascular endothelial growth factor (VEGF) of ADUB was obviously lower than that of the controls (P<0.05). The intensity of protein peak 13.08, 13.85, and 14.20 was not different between the cases and controls. SAA expressed highly in the menses of ADUB but low in that of the controls. Conversely, VEGF expressed highly in the menses of the control but low in that of the ADUB. Conclusion Two biomarkers which might be related with ADUB have been correctly screened and identified as SAA and VEGF. It needs further study whether the increased expression of SAA and reduced expression of VEGF are the cause or result of ADUB.

Objective To study the role of schizophrenia health education in intervening relapse of schizophrenia. Method 78 patients with schizaphrenia have been intervened by useing SHE about their staying in hospital and conditions out of hospitals after having recovered for three years, then compared with 85 schizophrenics receiving no SHE. Result It was clear that general rate of relapse in the study group was significantly lower than that of the control group. The patients obey medication and recovery of social function in the study group were significanly better than that in the controlgroup. Conclusion The SHE plays an important role in intervening relapse of schizophrenia and improving complete rehabilitation of schizophrenic.