Objective To investigate the inhibitory effect of celebrex on non-small cell lung cancer and its mechanisms.Methods the inhibitory effect of celebrex on NSCLC were detected by MTT Flow cytometry, electron microscope were used for evaluation of apoptosis and cell cycle block. The expression of P27 KIP1, XIAP were detected by reverse transcription-polymerase chain reaction.Results 1. celebrex inhibited cell survival of NSCLC in a time-dependent and concentration-depended manner, and the effect of celebrex were more pronounced in NCI-H520 than in A549.2.Flow cytometry show that celebrex induced a G0/G1 cell arrest in NSCLC. 3. The result of these apoptosis test' indicate that celebrex caused apoptosis in concentration-depended manner. 4.celebrex increased the expression of P27 KIP1,and decreased the expression of XIAP.Conclusions celebrex inhibited cell survival of NSCLC.Its mechanisms involved in cell cycle arrest and apoptosis.

Asian Continental Ancestry Group ; Hepatitis B virus ; Hepatitis B, Chronic ; immunology ; Humans ; Killer Cells, Natural ; immunology

The appliance of bilingual teaching of Medical Immunology offers an effective means of mastery and communication of the medical essential subject.This study has tried the bilingual teaching of medical immunology for clinical,dentistry and phylaxiology specialty students,discussed the model of bilingual teaching,investigated the teaching effect,analyzed and summarized the teaching experience.After the practice of bilingual teaching,we found that the feasible method of bilingual teaching to apply Chinese as the primary language and pervade English into the medical immunology course.

Endogenous beta retroviruses (enJSRV) are highly homologous with Jaagsiekte sheep retrovirus (exJSRV),this exogenous retrovirus is the aetiological agent of ovine pulmonary adenocarcinoma (OPA).The aim of this study was to clarify the function of enJSRV and the immunological mechanisms of its corresponding antibody,that is undetectable in JSRV-infected ovine serum.The expression of enJSRV envelope protein and Hyal-2 mRNA in immune organs and lungs of ovine fetuses and lambs were analyzed by Real-Time reverse transcription PCR and In Situ Hybridization using specific probes.In Situ Hybridization results indicated that the enJSRV envelope protein and Hyal-2 mRNA were expressed in thymus,spleen,mesenteric lymph nodes and lungs at different times,while no positive signals were detected in the negative controls.On the other hand,results from Real-Time reverse transcription PCR analysis showed that in 130d fetuses and 3d newborn lambs the enJSRV mRNA levels were much higher in organs associated with the immune system than that in lungs,especially in the thymus and spleen,but levels of Hyal-2 mRNA expression was not significantly different in all collected tissue.These results provided evidence from an immunology point of view to understand why the circulating antibodies against exJSRV are undetectable in JSRV-infected ovine,and will help to unravel the pathogenesis of JSRV-infected ovine.

Objective To evaluate the contraception efficacy, mode of bleeding, side effects and other positive effects of drospirenone-ethinylestradiol (Yasmin) in healthy Chinese women. Methods This was a multicenter, randomized, control study of 768 healthy Chinese women who consulted about contraception. The subjects were randomized into Yasmin group (30 μg ethinylestradiol plus 3 mg drospirenone, 573 cases) or desogestrel group (30 μg ethinylestradiol plus 150 μg desogestrel, 195 cases) with the ratio of 3: 1. Each individual was treated for 13 cycles. Further visits were required at cycle 4, cycle 7, cycle 10 and cycle 13 of treatment. Weight, height, body mass index were evaluated at each visit. The menstrual distress questionnaire (MDQ) was given to the women at baseline, visit 3 (cycle 7) and visit 5 (after cycle 13). Results The values of basal features were similar between two groups (P> 0.05). The Pearl index (method failure) of Yasmin was 0. 208/hundred women year which was lower than that of desogestrel (0. 601/hundred women year). The mode of bleeding was similar between two groups after trial without showing any significant difference. According to MDQ subscale, the improvement of water retention and increasing appetite during inter-menstrual period and water retention and general well-being during menstrual period in the Yasmin group ( -0. 297, -0. 057, 0. 033, 0. 150 respectively) was more obvious than that in the desogestrel group ( - 0. 108, 0. 023, 0. 231, - 0. 023 respectively) with a significant difference (P < 0. 05 ). Some other values which improved in beth two groups, especially the improvement of breast tenderness and pain and skin abnormality in Yasmin group (18.0%, 89/494; 12. 6%, 62/494) was more distinct than that in desogestrel group (11.3%, 19/168; 5.4%, 9/168). The mean weight increased in desogestrel group (0. 57 kg) while it decreased in Yasmin group ( -0. 28 kg) with a significant difference (P < 0. 01 ). Conclusions Both Yasmin and desogestrel have good efficacy on contraception and similar modes of menstrual bleeding. Yasmin is better than desogestrel in terms of weight control and premenstrual syndrome of oral contraceptive.

Objective To investigate the expression of Situin 1 ( SIRT1) in 5 strains of human lung adenocarcinoma cell lines, inclu-ding HCC827, H1650, H1975, A549 and H1299, and its relation to the susceptibility of nedaplatin ( NDP ) . Methods The SIRT1 mRNA and protein levels in 5 strains of human lung adenocarcinoma cells were detected by real-time quantitative PCR and Western blot, respectively. The viability of cells treated with NDP was detected by the CCK-8 method and the half growth inhibition concentra-tion ( IC50 ) was calculated. After the expressions of SIRT1 in A549, H1299, H1650 and H1975 cells were down-regulated by the siR-NA interference, the effects of NDP on the viability and apoptosis of these cells were determined by the CCK-8 method and flow cytom-etry, respectively.Results The expression levels of SIRT1 mRNA (4.53 ± 0.74, 3.11 ± 0.64, 15.76 ± 2.28 and 18.09 ± 1.17) and protein (0.23 ± 0.03, 0.21 ± 0.02, 0.52 ± 0.11 and 0.56 ± 0.08) in H1650, H1975, A549 and H1299 cells were significantly higher than that in HCC827 cells (1.00 for SIRT1 mRNA and 0.11 ± 0.02 for SIRT1 protein, F=122.10 and 26.50, respectively, P<0.01). The susceptibility of A549 and H1299 cells to NDP [IC50=(7.38 ± 1.59) and (8.14 ± 1.43) μmol/L, respectively] was significantly higher than that of HCC827, H1650 and H1975 cells [IC50=(26.16±4.35),(22.29±3.26) and (24.41 ± 2.58), respectively, F=30.86, P<0.01].The survivals of A549 and H1299 cells transfected by siSIRT1 and treated with NDP were significantly higher than that in the NC group ( F=235.10 and 39.20, respectively,P<0.01) , and the apoptotic rates were the reverse ( t=7.29 and 6.68, re-spectively, P<0.05) . However, the survivals of H1650 and H1975 cells transfected by siSIRT1 and treated with NDP were significantly lower than that in the NC group ( F=185.40 and 60.09, respectively,P<0.01) , and the apoptotic rates were the reverse ( t=6.15 and 31.36, respectively,P<0.01).Conclusion The expression of SIRT1 in A549 and H1299 cells with high expression of SIRT1 increases their susceptibility to NDP , while that in H1650 and H1975 cells with moderate expression of SIRT1 decreases their susceptibility to NDP, indicating that SIRT1 may play dual roles in the resistance of human lung adenocarcinoma cells to platinum.

Objective:To investigate the occurrence of geriatric syndromes in elderly inpatients with different grip strength levels and the effect of grip strength on geriatric syndromes and thus to provide insight for the management of geriatric syndromes in elderly inpatients.Methods:A total of 225 elderly inpatients who had received comprehensive geriatric assessment at the geriatrics department of our hospital were selected retrospectively, and their general and geriatric syndrome data were collected.With grip strength of 28 kg for men and 18 kg for women as the cut-offs, the patients were divided into the normal grip strength group(114 cases)and the decreased grip strength group(111 cases). Based on demographic characteristics including age, gender, type of work, income, educational background and social support, 1∶1 propensity score matching(PSM)for the two groups was conducted, successfully resulting in a total of 77 pairs.Differences in geriatric syndromes between the two groups were compared, and the relationship between grip strength and geriatric syndromes was analyzed in these elderly inpatients.Results:After adjustment for the general demographic characteristics by PSM, the decreased grip strength group showed higher rates than the normal grip strength group of frailty/pre-frailty(87.0% vs.55.8%), disability(53.2% vs.27.3%), malnutrition/malnutrition risk(57.1% vs.22.1%), cognitive impairment(35.1% vs.9.1%), constipation(44.2% vs.20.8%), falls(32.5% vs.13.0%)and number of geriatric syndromes(4, range: 2-5 vs.2, range: 0-4)and higher Carlson comorbidity index(CCI)scores(6, range: 5-8 vs.5range: 4-6)and higher body mass index scores[(25±3)kg/m 2vs.(23±4)kg/m 2](all P<0.05). There was no significant difference in the incidence of pain, sleep disorders, depression, anxiety or polypharmacy, or in short physical performance scores between the two groups(all P>0.05). Spearman correlation analysis showed that decreased grip strength was positively correlated with the incidence of frailty/pre-frailty( r= 0.345), disability( r= 0.265), malnutrition/malnutrition risk( r= 0.358), cognitive impairment( r=0.313), constipation( r= 0.250), falls( r= 0.232)and number of geriatric syndromes( r=0.370)(all P<0.05). There was no significant correlation between grip strength and the incidence of pain, sleep disorder, depression or anxiety(all P> 0.05). Multivariate logistic regression analysis showed that, compared with the normal grip strength group, the decreased grip strength group had a higher risk of developing geriatric syndromes such as frailty/pre-frailty( OR=10.906), disability( OR=4.025), malnutrition/ malnutrition risk( OR=2.699), cognitive impairment( OR=6.620), constipation( OR=2.848)and falls( OR=4.145, all P<0.05). Conclusions:Decreased grip strength is an independent risk factor for many common geriatric syndromes such as frailty/pre-frailty, disability, malnutrition/ malnutrition risk, cognitive impairment, constipation and falls.Elderly patients with decreased grip strength should be a key population group when screening for geriatric syndromes.

Objective To investigate the distributions of genotypic and allelic frequencies of intercellular adhesion molecule-1 (ICAM-1) gene K469E and platelet endothelial cell adhesion molecule-1 (PECAM-1) gene C373G in patients with preeclampsia. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and DNA sequencing were used for detecting ICAM-1 gene K469E and PECAM-1 gene C373G genotypes in 110 women with preeclampsia and 110 normotensive pregnant women in comparison with their clinical characteristics. Results The distributions of observed and expected genotype frequencies were consistent with Hardy-Weinberg equilibrium. No significant differences were found in the genotype and allele frequencies of ICAM-1 gene K469E between the two groups (P>0.05), but the CC and the CG genotype frequencies of PECAM-1 gene C373G were significantly different between them (P<0.05). The relative risk for preeclampsia of CG genotype was 1.959 folds of that in CC genotype carriers (OR=1.959, 95%CI:1.090-3.520, P=0.024), and this association still existed after adjustment for age, gravidity, parity and BMI in logistic regression models. The C373G allele frequencies showed no significant difference between the two groups (P>0.05). Conclusions The CG genotype of PECAM-1 gene C373G genetically predispose the carriers to preeclampsia, while ICAM-1gene K469E polymorphisms is not associated with preeclampsia.

Objective To investigate the distributions of genotypic and allelic frequencies of intercellular adhesion molecule-1 (ICAM-1) gene K469E and platelet endothelial cell adhesion molecule-1 (PECAM-1) gene C373G in patients with preeclampsia. Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and DNA sequencing were used for detecting ICAM-1 gene K469E and PECAM-1 gene C373G genotypes in 110 women with preeclampsia and 110 normotensive pregnant women in comparison with their clinical characteristics. Results The distributions of observed and expected genotype frequencies were consistent with Hardy-Weinberg equilibrium. No significant differences were found in the genotype and allele frequencies of ICAM-1 gene K469E between the two groups (P>0.05), but the CC and the CG genotype frequencies of PECAM-1 gene C373G were significantly different between them (P<0.05). The relative risk for preeclampsia of CG genotype was 1.959 folds of that in CC genotype carriers (OR=1.959, 95%CI:1.090-3.520, P=0.024), and this association still existed after adjustment for age, gravidity, parity and BMI in logistic regression models. The C373G allele frequencies showed no significant difference between the two groups (P>0.05). Conclusions The CG genotype of PECAM-1 gene C373G genetically predispose the carriers to preeclampsia, while ICAM-1gene K469E polymorphisms is not associated with preeclampsia.

OBJECTIVETo investigate the distributions of genotypic and allelic frequencies of intercellular adhesion molecule-1 (ICAM-1) gene K469E and platelet endothelial cell adhesion molecule-1 (PECAM-1) gene C373G in patients with preeclampsia.

METHODSPolymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and DNA sequencing were used for detecting ICAM-1 gene K469E and PECAM-1 gene C373G genotypes in 110 women with preeclampsia and 110 normotensive pregnant women in comparison with their clinical characteristics.

ESULTSThe distributions of observed and expected genotype frequencies were consistent with Hardy-Weinberg equilibrium. No significant differences were found in the genotype and allele frequencies of ICAM-1 gene K469E between the two groups (P>0.05), but the CC and the CG genotype frequencies of PECAM-1 gene C373G were significantly different between them (P<0.05). The relative risk for preeclampsia of CG genotype was 1.959 folds of that in CC genotype carriers (OR=1.959, 95%CI: 1.090-3.520, P=0.024), and this association still existed after adjustment for age, gravidity, parity and BMI in logistic regression models. The C373G allele frequencies showed no significant difference between the two groups (P>0.05).

CONCLUSIONSThe CG genotype of PECAM-1 gene C373G genetically predispose the carriers to preeclampsia, while ICAM-1gene K469E polymorphisms is not associated with preeclampsia.

Adult ; Case-Control Studies ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Intercellular Adhesion Molecule-1 ; genetics ; Platelet Endothelial Cell Adhesion Molecule-1 ; genetics ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length ; Pre-Eclampsia ; genetics ; Pregnancy ; Sequence Analysis, DNA