1.Acid Copper-Containing Waste Water Treatment by Eggshells:an Experimental Study
Xiaoyan LI ; Shuyin YIN ; Wenjuan LI
Journal of Environment and Health 2007;0(11):-
Objective To find a simple, effective and inexpensive method for acid copper-containing waste water treatment. Methods Studied the processing of the leaching waste water from the laboratory of East China Institute of Technology and simulated acid copper mine waste water(concentration of Cu2+ is 143.00 mg/L, pH: 1.80-2.00) using the processed eggshells to treat acid copper-containing waste water under the condition of different calcining temperature, eggshells amount,eggshells size and stirring speed. Results The best condition was that the calcining temperature was 400 ℃, the amount of eggshells was 25 g/L, the size of eggshells was 0.25 mm, the stirring speed was 240 r/min. The eggshells could increase the pH values from 1.80-2.00 to 6.86-7.34 and reduce Cu2+ concentration from 0.43 mg/L to 0.09 mg/L, the concentration of Cu2+ in the treated waste water could answer for the first level of the Integrated Waste Water Discharge Standard(GB 8978-1996), the removal rates could reach 99.70%-99.94%. Conclusion Using eggshells to treat acid copper-containing waste water has application prospects for the process, is simple and easy to be operated, the treatment effect is good.
2.Establishment of Model of Dynamic Change of Immune Status of ACLF Induced by ConA Repeated Administration in Mice
Nannan ZHANG ; Shuyin YANG ; Liuying CHEN ; Shan YIN ; Shijin WANG ; Sanhai LIU ; Beibei WANG ; Zheng WANG ; Hai LI
Chinese Journal of Gastroenterology 2016;21(6):326-330
Background:Acute-on-chronic liver failure( ACLF)is a commonly seen liver failure in China,and lacking an animal model that can effectively simulate the dynamic change of immune status of ACLF. Aims:To establish an animal model that can simulate dynamic change of immune status of ACLF by repeated administration of concanavalin A(ConA). Methods:Mice were randomly divided into normal control group and ConA repeated administration group. Mice in ConA repeated administration group were injected with ConA 15 mg/ kg through retrobulbar angular vein every 48 hours for 5 times,and mice in control group were injected with same volume of 0. 9% NaCl solution. Serum levels of IL-6,IL-10,IL- 12,TNF-α,IFN-γ,MCP-1 in peripheral blood were assessed by CBA assay,and the ratio of IL-10/ TNF-α was calculated. The expression of HLA-DR,number and proportion of CD4+ T cells and the expression of PD-1 of monocytes in peripheral blood were detected by flow cytometry. Results:Peripheral blood cytokines changed from predominated proinflammatory cytokines into predominated anti-inflammatory cytokines with the increasing in time of administration in ConA repeated administration group. Compared with control group,HLA-DR expression of monocytes in peripheral blood was significantly decreased(P <0. 05),number and proportion of CD4+ T cells were significantly decreased(P <0. 05), and PD-1 expression was significantly increased( P < 0. 05)in ConA repeated administration group. Conclusions:An animal model of ACLF immune status from systemic inflammatory response syndrome( SIRS) to compensatory antiinflammatory response syndrome(CARS)induced by repeated ConA stimulation is successfully established.