ObjectiveTo investigate the effects of atorvastatin on cardiomyocyte apoptosis and caspase-3 expression in acute myocardial infarction in rats.MethodsFifty SD rats were randomly divided into 3 group:sham group,control group( normal saline),atorvastatin pretreatment group ( 10mg/kg),The atorvastatin group were pretreated with atorvastatin ( 10mg/kg ) for 3 days,and those in the control group were pretreated with normal saline instead.All rats underwent coronary ligation of left anterior descending branch to establish AMI rat s model on the fourth day except the sham group,while sham group only received thoracotomy without coronary ligation.Then,The infarct size area at risk were defined by Evans blue and TTC staining,the caspase-3 mRNA expression of myocardium was measured with reverse-transcriptase polymerase chain reaction(RT-PCR) and the apoptosis index was analyzed by TUNEL staining.Results Compared with control group,the infarct size,the caspase-3 mRNA expression of myocardium,the apoptotic index were decreased significant in the atorvastatin group ( P ＜ 0.05 ).ConclusionPretreatment with atorvastatin has protective effect on the acute ischemic cardiomyocytes,which may probably be associated with the reduction of the expression of caspase-3 mRNA in the myocardium and the apoptotic index.

From ethics angle analysis of the characteristics of the doctor -patient contradiction in the new peri-od, clear the hospital party committee in the political core position in the construction of a harmonious doctor -pa-tient relationship , and puts forward play the ethical function of the hospital party committee in building a harmoni -ous doctor-patient relationship , finally summarize the hospital party committee in rebuilding the harmonious doctor-patient relationship specific ethical measures , including: strengthening the ethical and moral education , in -depth professional moral education; Hospital ethics committee , and actively promote ethical operation system; To improve medical environment , mobilize the enthusiasm of the party branch;And improve the mechanism of doctor-patient communication , to protect patients′right to know and privacy;The treatment medicine , commercial brib-ery letter-inquiring-and-accusing work mechanism .

Objective To explore clinical value of detection of carcinoembryonic antigen(CEA) ,cytokeratin 19 fragment(CY-FRA21-1) and neuron specific enolization enzyme(NSE) in pleural effusion and serum during the differential diagnosis and patho-logical classification of lung cancer .Methods 180 patients with pleural effusion were divided into two groups ,one including 55 be-nign pleural effusion cases(benign pleural effusion group) and the other including 125 lung cancer cases(lung cancer group) .CEA , NSE and CYFRA21-1 in pleural effusion and serum of two groups were measured ,respectively ,and the measure results between two groups were conducted .Results CEA ,NSE and CYFRA21-1 levels in lung cancer group were significantly higher than those in benign pleural effusion group(P<0 .05) .CEA levels in pleural effusion and serum in lung adenocarcinoma group were significantly higher than those in benign pleural effusion group ,lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .CY-FRA21-1 levels in pleural effusion and serum in lung squamous carcinoma group were significantly higher than those in benign pleu-ral effusion group ,lung adenocarcinoma group and small cell lung cancer group(P<0 .01) .NSE levels in pleural effusion and serum in small cell lung cancer group were significantly higher than those in benign pleural effusion group ,lung adenocarcinoma group and lung squamous carcinoma group(P< 0 .01) .The positive detection rate of CEA in lung adenocarcinoma group was significantly higher than that in lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of CY-FRA21-1 in lung squamous carcinoma group was significantly higher than that in lung adenocarcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of NSE in small cell lung cancer group was significantly higher than that in lung squamous carcinoma group and lung adenocarcinoma group(P<0 .01) .Conclusion CEA ,CYFRA21-1 and NSE in pleural effusion and serum are specific tumor markers of lung adenocarcinoma ,lung squamous carcinoma and small cell lung cancer ,respectively , which may have a certain clinical significance for the differential diagnosis and pathological classification of lung cancer .

Objective To identify the incidence and risk factors associated with urinary tract infection in spinal cord injured patients who accepted sterile intermittent catheterization. Methods 243 patients with spinal cord injury were analysed retrospectively. Results The overall incidence of urinary tract infection was 0.93 episodes per 100 person-days. The risk factors associated with urinary tract infection were age older than 40, frequent exposure to antibiotics, cervical injury, score of functional independence measure less than 71 and bladder dysfunction. The independence risk factors were cervical injury, score of functional independence measure less than 71 and bladder dysfunction. Conclusion High level spinal cord injured patients with lower function and bladder dysfunction are higher risk for urinary tract infection.

目的了解目前康复医学进修人员的状况,为各医院选派进修人员提供依据。方法对我院1994—2001年687名康复医学进修人员的进修专业、职称等资料进行分析。结果进修人员呈逐年上升趋势,多分布在物理治疗科、作业治疗科等康复治疗科室,以初级职称为主。结论医院应对选派人员的进修专业统筹考虑,并注意人才梯队建设。

A lot of important information were hidden in mass LIS data.Data mining technology can find useful data from multiple dimensions,providing information support for clinical diagnosis and treatment.This paper described the newly gained features of the laboratory medicine in the era of big data and analyzed the potential application of data mining in laboratory medicine.

Objective To explore the possible relationship between the expression of CD14 and HLA-DR on peripheral blood monocytes and progress of the illness in neonatal infection. Methods To measure the expression of CD14 and HLA-DR on peripheral blood monocytes in the 1st, 5th and 7th days after the neonates were diagnosed as infectious diseases by flow cytometry of dual stai-ning techniques. Thirtyfour uninfected neonates were served as controls. Outcomes were analyzed. Results Neonates with infectious diseases had signi-ficantly lower serum CD14(P

Objective To investigate the mechanism of platelet inhibitor from Agkistrodon halys venom (AHV-PI) on platelet aggregation. Methods Protein kinase Akt phosphorylation levels in platelet were measured by Western blot. XS-1000I blood cell counter was used for platelet count. The plasma content of 5'-NT and platelet membrane GPIb were determined by Enzyme-Linked Immunosorbnent Assay (ELISA). The effect of AHV-PI on binding rate between the fluorescence labeled monoclonal antibody CD61 (FITC-CD61) and platelet membrae glycoprotein Ⅱb/Ⅲa (GPⅡb/Ⅲa) was observed by flow cytometry (FCM). Results AHV-PI can reduce the level of Akt-phosphorylation level and the number of platelet. AHV-PI can increase the content of 5'-NT in plasma, reduce the expression of platelet GPIb. Flow cytometry displayed AHV-PI can not affect the rate of combination between platelet GPⅡb/Ⅲa and FITC-CD61. Conclusion The mechanism of inhibition of platelet aggregation may be inhibit protein kinase Akt phosphorylation to block the signal transduction pathway of Akt. Limit cell grouth and reduce platelet number, also it may be related to its 5'-NT activity, it can degradate ADP to prevent the formation of platelet thrombus.

0.05), but BIS was markedly lower in group Ⅲ than that in group Ⅰ (P

Objective:To probe the therapeutic effects of Butylphthalide Injection in the elderly patients with acute cerebral infarction (ACI) and its influence in cerebral hemodynamics and cerebral vascular reserve (CVR),and to clarify the pharmacological action mechanism of butylphthalide in treatment of ACI.Methods:A total of 100 cases of elderly patients with ACI were selected as the subjects and divided into observation group and control group according to the serial number on admission.Fifty cases were included in each group.The patients in control group were treated with the conventional treatment, while the patients in observation group were treated with Butylphthalide Injection on the basis of the conventional treatment.The National Institute of Health Stroke Scale (NIHSS) score, the brain hemodynamics indexes of the peak velocity (Vp), the mean velocity (Vm) and the differences of the velocity (DVp, DVm) as well as pulsatility index (PI), CVR of bilateral middle cerebral artery (MCA)of the patients in two groups were observed and compared.The therapeutic effects of the patients in two groups were evaluated and compared.Results:The NIHSS score of the patients in observation group after treatment was significantly lower than that in control group (t=15.420, P<0.05).The therapeutic effects and the clinical efficiency of the patients in observation group were significantly better than those in control group (U=2.225, χ2=5.005, P<0.05).The Vp and Vm of the patients in observation group after treatment were significantly higher than those in control group(t=10.819,t=7.259, P<0.05)and the DVp and DVm were significantly lower than those in control group (t=16.438,t=19.055, P<0.05).The CVR of the patients in observation group after treatment was significantly higher than that in control group(t=6.884, P<0.05)and the PI was significantly lower than that in control group (t=4.979, P<0.05).Conclusion:Butylphthalide Injection can effectively correct the abnormality of brain hemodynamics in the ACI patients, enhance the ability of body in maintaining the stability of cerebral vascular perfusion, improve the neurological symptoms in the patients with ACI, and improve the therapeutic effects.