1.Changes of Oscillatory Potentials of Electroretinogram after Methanol Intoxication in Rats
Dongmei LIU ; Shu ZHOU ; Jiemin CHEN ; Shuya PENG ; Wentao XIA
Journal of Forensic Medicine 2014;(3):178-180
Objective To study the characters of oscillatory potentials (OPs) of electroretinogram (ERG) after methanol intoxication in rats. Method The SD rat models of methanol intoxication were established and divided into control group, 3-day intoxication group, 7-day intoxication group. The changes of OPs of ERG were recorded in a dark room. Results The total amplitudes of 3-day and 7-day intoxication groups decreased approximately 50% compared with that of the control group, while the schedule de-layed approximately 16% and 61%, respectively. Conclusion The characters of methanol intoxication in rats included delay in schedule and decline in the total amplitude of OPs.
2.Expression and Mechanism of αB-crystallin in Retina and Extraocular Tissues and Organs
Dongmei LIU ; Shu ZHOU ; Jiemin CHEN ; Shuya PENG ; Wentao XIA
Journal of Forensic Medicine 2014;(6):470-473
αB-crystallin is the structural protein of vertebrate lens, w hich is w idely expressed in non-lens tissue. As one of the heat shock protein fam ily m em bers,αB-crystallin possesses biological proper-ties of m olecular chaperones and anti-apoptotic effects. Multi-factor injuries, such as retinopathy, inflam-m ation and nervous system diseases, have a closely relationship w ith αB-crystallin. T his paper review s the research progress of the expression and m echanism ofαB-crystallin in retina and extraocular tissues and organs.
3.Forensic Application of Microperimetry and Visual Evoked Potential in Macular Disease
Shu ZHOU ; Dongmei LIU ; Shuya PENG ; Jing SUN ; Ruijue LIU ; Wentao XIA
Journal of Forensic Medicine 2015;(2):105-108
Objective To find the correlation between real best corrected visual acuity (BCVA) and test-ing results of microperimetry and visual evoked potential (VEP) and to explore a newmethod in record-ing BCVAin macular disease. Methods Sixty-two patients with macular disease (macular disease group, 62 eyes) and eighteen healthy volunteers (control group, 36 eyes) had BCVA, microperimetry and VEP recorded. Results (1) By microperimetry, the values of retinal mean sensitivity and fixation percentage in macular disease group were lower than that in control group. The bicurve ellipse area in macular dis-ease group was higher than that in control group. By V EP, P100 amplitude under 0.5 cpd and 2 cpd in macular disease group were significantly higher than that in control group and the latency was prolonged (P<0.05). (2) In macular disease group, BCVAhad significant positive correlation with retinal mean sen-sitivity, bicurve ellipse area, macular central 2°and 4°fixation percentage, respectively (P<0.05). There was a significant correlation between retinal mean sensitivity and P100 amplitude (P<0.05). (3) multiple linear regression equation was y=0.053 x1+0.008 x3+3.897 (y was BCVA, while x1 was retinal mean sensi-tivity and x3 was P100 amplitude under 2 cpd). Conclusion C ombined use of microperimetry and VEP is useful in the assessment of BCVAin macular disease.
4.The Injury Mechanisms of Fracture due to Domestic Violence: A Case Study
Dongmei LIU ; Dong GAO ; Wentao XIA ; Zhengdong LI ; Shu ZHOU ; Shuya PENG
Journal of Forensic Medicine 2017;33(2):209-213
T his article reported a 37-year-old w om an w ho w as physically attacked by her husband, w hich caused her leg injuries. In the hospital a diagnosis w as m ade of fractures of the right patella and left tibia before she underw ent an operation of open reduction and internal fixation. B ecause the husband re-fused to adm it his fam ily violence com m itted against this w om an; instead he claim ed that her low er lim b fractures belonged to the falling injuries caused by a traffic accident. T he police assigned forensic experts to judicially judge the w om an's injuries. A nd the identification results of X-ray and C T show ed that the transverse fracture of patella had been caused by m uscular violence, and that both direct and indirect force resulted in the injuries of left tibia, and the fracture of left m edial orbital w all m ight have been form ed during the violence. T his article expounded the identification betw een the injuries of fam ily violence and traffic accident by the applications of im aging, w hich em phasized the application value of im aging techniques in clinic forensic science.
5.Correlation between Contrast Vision and Sweep Visual Evoked Potential Acuity
Shuya PENG ; Jiemin CHEN ; Dongmei LIU ; Shu ZHOU ; Meng WANG ; Wentao XIA
Journal of Forensic Medicine 2015;(1):20-22,27
Objective To compare the correlation between contrast vision (CV) and sweep visual evoked potential acuity (SVEP-A) among people with emmetropia, mild myopia, and moderate myopia. Meth-ods The CV and SVEP-A were tested individually in 96 eyes from healthy young volunteers, including 37 eyes of emmetropia, 27 eyes of mild myopia, and 32 eyes of moderate myopia. The statistic analysis was done by ANOVA analysis and rank sum test. Results (1) With the decrease of contrast, CV and SVEP-A decreased in every group. (2) At 100% contrast, the difference of CV between emmetropia and mild myopia had statistical significance (P<0.05). At 100%, 25% and 10% contrast, the difference of CV between emmetropia and moderate myopia had statistical significance (P<0.05). (3) In the same group, the difference of 100% and 25% contrast had statistical significance (P<0.05). So was between 100% and 10% contrast. (4) At 100% and 10% contrast, the difference of CV and SVEP-A had statis-tical significance (P<0.05). Conclusion The CV of myopia relates to many factors including ametropia and fundus lesions. The correction of ametropia is important to the values of CV and SVEP-A.
6.Characterization of growth performance of F1 hybrid CB6 F1 C57-ras transgenic mouse model
Susu LIU ; Xi WU ; Shuya ZHOU ; Chenfei WANG ; Zexu PENG ; Qin ZUO ; Baowen LI ; Zhengming HE ; Changfa FAN
Chinese Journal of Comparative Medicine 2015;(4):18-22
Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras+/-) male mice and wild-type BALB/cJ female mice.The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed.Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction.The average body weight of newborn mice was 1.73 ±0.05 g.The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38 ±1.74 g and 29.42 ±1.72g, respectively, which had a significant difference (P<0.01).The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules.Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic ( c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.
7.Breviscapine Ameliorates Fear Extinction and Anxiety in BALB/cJ Mice
Wei LIANG ; Jing HUANG ; Shuya YANG ; Peng HUANG ; Jun CHEN ; Chen CHEN ; Qun YANG ; Fengzhan LI
Psychiatry Investigation 2023;20(3):205-211
Objective:
In this research, the influence of breviscapine on anxiety, fear elimination, and aggression and the potential mechanism was investigated.
Methods:
Anxiety and locomotion were analyzed by elevated plus maze and open field test in mice. Bussey-Saksida Mouse Touch Screen Chambers were used to perform fear conditioning. Territorial aggression was assessed by resident intruder test. Protein levels were evaluated by Western blot. Breviscapine improved fear-extinction learning in BALB/cJ mice.
Results:
Breviscapine at 20–100 mg/kg increased center cross number, total distance traveled, and velocity in a dose-dependent manner. On the other hand, breviscapine at 20–100 mg/kg decreased the immobility time in open field test. In addition, breviscapine at 20–100 mg/kg increased the ratio of time on the open arm, time on the distal parts of the open arm, and total distance traveled in elevated plus maze. Breviscapine at 100 mg/kg increased the average attack latency and decreased the number of attacks over the last 3 days of resident intruder test. In hippocampus, protein levels of postsynaptic density protein-95 and synaptophysin were elevated by breviscapine at these three doses.
Conclusion
The administration of breviscapine alleviates fear extinction, anxiety, and aggression, while increases locomotor in a dose-dependent manner, which might be associated with its influence on synaptic function.
8. The impact of resection of skull base tumor via an endoscopic endonasal approach on the visual function of vision impaired patients and the analysis of factors affecting their visual recovery
Zhenlin WANG ; Qiuhang ZHANG ; Mingchu LI ; Bo YAN ; Tingting WEI ; Shuya PENG ; Pu LI ; Lianjie CAO
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2018;53(4):244-250
Objective:
To evaluate the visual outcomes of patients with visual impairment after resecting skull base tumor via an endoscopic endonasal approach, and to analyze the factors affecting visual recovery.
Methods:
One hundred and fifty-three patients with skull base tumor who suffered from preoperative visual impairment from Skull Base Surgery Center of Xuanwu Hospital were operated through an endoscopic endonasal approach. Both preoperative and postoperative visual function outcomes as well as factors that might have affected their visual recovery were analyzed retrospectively by
9.Understanding the phase separation characteristics of nucleocapsid protein provides a new therapeutic opportunity against SARS-CoV-2.
Dan ZHAO ; Weifan XU ; Xiaofan ZHANG ; Xiaoting WANG ; Yiyue GE ; Enming YUAN ; Yuanpeng XIONG ; Shenyang WU ; Shuya LI ; Nian WU ; Tingzhong TIAN ; Xiaolong FENG ; Hantao SHU ; Peng LANG ; Jingxin LI ; Fengcai ZHU ; Xiaokun SHEN ; Haitao LI ; Pilong LI ; Jianyang ZENG
Protein & Cell 2021;12(9):734-740