Transpupilary Argon laser photocoagulation of the ciliary processes was performed on 26 eyes of refractory glaucoma. Postoperative intraocular pressure was under 2.74 kPa (21 mmHg) in 13 eyes. As a cyclo-destructive surgery, the present modality showed the merits of safety, less pain and fewer complications than cyclocryotherapy.

The pre-and post-operative evaluation of ocular anterior segment with glaucoma using ultrasound bio- microscopy(UBM)was introduced.We performed UBM on 21 eyes of 17 patients,mainly for the de- tection of flat anterior chamber,cilio-choroidal de- tachment,malignant glaucoma,and the state or func- tion of the filtering bleb after filtering surgery.In the secondary glaucoma with dense corneal scars,by UBM,reliable high-resolution images delineating ana- tomic relationships and pathologic changes in the inner eye can be obtained.This information aids in surgical planning,and increases the successful rate of the glaucoma surgery.In addition,UBM is also useful in the localization and determination of the extent of ciliary body dialysis after contusion.In our initial experience,UBM is worthy in the study of the pathogenesis of glaucoma,selection of surgical cases,observation of complications and long-term postoperative follow-up.

It is not rare for nanophthalmos secondary glaucoma that filtration operation results in blindness,especially after the filtration operation is more popular,but surgical skill is not enough even for every surgeon.Thus the eye axis measurement is empha- sized for patients with angle closure glaucoma in order to affirm whether it is nanophthalmos,and a simple three-step method is intro- duced to recognize nanophthalmos quickly.We should stress the general detail design for treatment of nanophthalmos secondary glauco- ma.It should be careful to use miotic agents during its treatment.If have to do surgeries,we should know their high risks and can cor- rectly deal with complications such as malignant glaucoma,uveal effusion,choroid detachment,expulsive hemorrhage and so on.Its sur- gical fashions will depend on pathophysiological mechanisms and structure of ocular anterior segment of patients with nanophthalmos. Good communications with patients and their relatives and excellent operative skills are important factors for ensuring successful surgeries.

Male Wistar rats were used to investigate the ultrastructure of the fibroblasts. The animals were sacrified at several time intervals after the collodion tubules were embedded subscutaneously on the backs, and the materials taken from the connective tissues around the collodion tubules were observed by light and electron microscopy. Six basic types of fibroblasts (little-differentiated fibroblast, young fibroblast, collagenoblast, myofibroblast, fibroclast and fibrocyte) were studied ultrastructurally. In each of its three phases (prophase, metaphase and telophase), the collagenoblast showed different functional conditions. The fibroclasts contain two subtypes, the phagocytosis and the lysosome subtypes, the former phagocytosing the collagen fibrils with a characteristic crossing banding and the latter phagocytosing the collagen fibrils which had been cleaved by the collagenase. The fibrocytes consist probably of two subtypes, the resting and aging subtypes. The observations demonstrate that the fibroblasts were derived from undifferentiated cells around capillaries and the little-differentiated fibroblasts are the youngest cell type of all fibroblasts. The collagenoblasts and fibroclasts are the main cells responsible for collagen biosynthesis and collagen degradation respectively. In the present paper a new concept, the "The Fibroblast System", has been proposed, which includes the six basic types of fibroblasts and there are not only structural but also functional relationships among them.

ObjectiveTo investigate the effect of Buchangwenxin particles in maximum P wave (Pmax), dispersion of P wave(Pd) of elder patients with diastolic cardiac dysfunction and paroxysmal atrial fibrillation(PAF).Methods115 elder patients with diastolic cardiac dysfunction and PAF were randomly divided into two groups:group A received the routine drug therapy,and group B received Buchangwenbxin particles with routine drug therapy.Another 54 elder patients with diastolic cardiac function but without PAF was selected as control group.Changes in Pmax and Pd were compared between groups with or without PAF as well as subgroups.ResultsPmax and Pd were wider in PAF group than no PAF group(all P ＜0.01).There was no difference between group A and B in Pmax and Pd (all P＞ 0.05), and there were less atrial fibrillation as well as shortened Pmax and Pd in group B, which was significantly different with group A(all P ＜0.01).ConclusionPmax and Pd were wider in elder patients with diastolic cardiac dysfunction and PAF than those without PAF,and Buchangwenxin particles could shorten Pmax and Pd and reduce the attacks of atrial fibrillation.

Objective:To explore the concentration of monoamine neurotransmitter in serum and psychiatric symptoms such as anxiety and depression in infertility patients with and without PCOS(No-PCOS),and the relationship between them.Methods:30 infertility with PCOS were selected as experimental group(EG) and 30 infertility patients without PCOS as control group(CG).They were administered Symptom Checklist(SCL-90).Their monoamine neurotransmitter in serum were determined including norepinephrine(NE),3-methoxy-4-hydroxyphenylglycol(MHPG),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA),dopamine(DA),homovanillic acid(HVA) and dihydroxy-phenyl acetic acid(DOPAC).Results:The findings indicated that the scores in anxiety and depression subscales of SCL-90 were significantly higher in the PCOS group than those in the No-PCOS(P

Objective: To evaluate the effect of Chinese herbal medicine on Chronic Periodontitid.(CP).Method:34 patients with CP were randomly divided into two groups.The herbal medicine was given to the experiment group for two months,while the other group was set as a blank control.The Periodontitid and laboratory test indexes such as PLP,PPDPAL,MD and Ca P,ALP of patients were measured before and after treatment.Results: The indexes of patients in the group of Chinese herbal medicine were statistically signifi cant in contrast with the control group(P

Objective To explore the clinical effect of cholangioscopic Holmium laser lithotripsy for bile duct refractory residual stone and orthomorphia for intrahepatic bile duct membranous stenosis. Methods The final diagnosis was established by T tube retrograde cholangiography and cholangioscopy in 12 patients with refractory bile duct residual stone. Twenty-nine stones distribute in extrahepatic and intrahepatic bile duct and 5 intrahepatic bile duct membraneous stenosis were found in 3 cases. All patients underwent Holmium laser lithotripsy and orthomorphia. Results The stone clearance rate was 100% in 12 patients after cholangioscopic holmium laser lithotripsy. No serious complication occurred in patients after 5 membranous stenosis orthomorphia. Follow-up observation on 11 patients shows no obvious recurrence of symptom and stones. Conclusion Holmium laser lithotripsy and orthomorphia are safe and effective in the treatment of bile duct refractory residual stone and stenosis.

Objective To elucidate the relationship between LYVE-1 and VEGF-C and their expression in laryngeal squamous cell carcinoma(LSCC),and provide theoretic evidence for the judgement of metastasis and prognosis of LSCC,also for the treatment.Methods An immunohistocbemical analysis was performed to 50 specimens of LSCC with lymphatic endothelial marker LYVE-1.Quantitation of lymphangiogenesis growth factor VEGF-C by RT- PCR was performed to 30 specimens of LSCC.Finally the correlation between LVD and VEGF-C mRNA was analyzed with statistics methods.Results LYVE-1 (+) was observed in all LSCC.The median copy number of VEGF-c mRNA was 4-5-fold higher in LSCC than in adjacent normal tissue.There was correlation between tumor VEGF-C mRNA copy number and intratumoral LVD.Conclusion Lymphatic vessels existed in LSCC.There was correlation between high levels of LVD in LSCC than in normal tissue.The high level of VEGF-C may accelerate the lymphatic metastasis by promoting the proliferation of lymphatic vessel.

Objective To investigate the length of preserved ileocecum in surgical treatment of slow transit constipation ( STC) by sub-total colectomy with antiperistaltic cecorectal anastomosis .Methods A total of 82 patients with STC were divided into two groups according to the random number table method ,with 41 cases in each group ,all the patients of the two groups underwent subtotal colectomy ,intraopera-tive ileocecum was preserved length of group A was 10~15 cm,group B was 2~3 cm.The operation time,intraoperative bleeding volume, postoperative exhaust time and length of hospital stay were compared .Wexner constipation score and gastrointestinal quality of life index ,ab-dominal pain ,frequency score and emptying time of ileocecus before and after 6 months and 12 months between the two groups were com-pared.Results There was no statistically significant difference in the operation time ,intraoperative bleeding volume ,postoperative exhaust time and hospitalization time of two groups (P>0.05).Wexner constipation score,abdominal pain,frequency score of the two groups after 6 months and 12 months decreased significantly (P<0.05,P<0.01),of which the group B was lower than that of the group A (P<0.05,P<0.01). The gastrointestinal quality of life index after operation significantly increased (P<0.01),of which the group B was higher than that of the group A (P<0.01).Ileocecal emptying time of the group B 12 months after operation was shorter than that of the group A (P<0.01),the differences were statistically significant .Conclusion Subtotal colectomy with antiperistaltic cecorectal anastomosis is an effective method to treat STC,which can reduce the length of preserved ileocecum and improve the prognosis of patients .