Protective effects of Ginkgo biloba extract (GBE) on ischemia in rat and mice brain were studided. The ischemia model in brains was prepared by occlusion of bilateral carotid arteries and vertebra arteries in rat. The results demonstrated that GBE had no effect on electroencephalogram (EEG) of 5 min of ischemia, but it promoted the recovery of EEG during reperfusion. When it was reperfushed for 10 min,amplitude of EEG in the control group only recovered from 17. 2% after ischemia to 46. 6% of normal level. While 0. 2g/kg GBE has a recovery of 93% from normal level. At 4. 0g/kg GBE the life-span of mice with bilateral ligated carotid arteries was significantly lengthened. The above results proved that GBE has some protective effect on anoxia caused by acute ischemia in rat and mouse brain.

Total cardiac glycosides isolated from dry fruit of Erysium chei- ranthoides L ( ETCG ) possesed positive inotropic effect. Its effects on the physiological properties of guinea pig left atria were studied. in this paper. The effects of ETCG on dose-response curve were investigated. The results showed that ETCG increased contractility of left atria significantly and dose-dependently .The pD2 value was 7.94. ETCG ( 0.17mg/L)increased significantly excitability and automaticity induced by epinephrine. ETCG(0.01mg/L ,0 .043mg/L ,0 .17mg/L)prolonged functional refractory period obviously.

0.05), but were highly significant at doses of 100 ?mol/L or above (P

Objective To explore the relationship between 24 h ambulatory blood pressure monitoring (24h-ABPM) parameters, circadian rhythm and urinary albumin to creatinine ratio (UACR), urineβ2 microglobulin (β2-MG) in elderly patients with hypertension. Methods One hundred and forty-eight patients with essential hypertension (≥60 years old) were included in this study. 24h-ABPM was performed, and nocturnal blood pressure decline rate (BPR) was calculated. The patients were divided into two groups according the BPR:dipper group with 40 cases and non-dipper group with 108 cases. The levels of 24h-ABPM parameters, UACR and β2-MG were compared between two groups, and the relationship between UACR and 24h-ABPM parameters were analyzed. Results The levels of 24hSBP, dSBP and 24hDBP in two groups had no significant differences (P>0.05). Compared with those in dipper group, the levels of nSBP, nDBP in non-dipper group were significantly increased, and the level of dDBP was significantly decreased, P<0.01 or <0.05. The level of 24hPP in non-dipper group was significantly higher than that in dipper group (P<0.01). The levels of UACR andβ2-MG in non-dipper group were significantly higher than those in dipper group (P<0.05). The levels of BUN and SCr in two groups had no significant differences (P>0.05). The result of Sspearman analysis showed that UACR had significant correlation with 24hDBP, nDBP, 24hSBP, nSBP and 24hPP (P<0.05 or<0.01). The result of multivariate linear regression analysis showed that UACR was independently correlated with nSBP (P<0.05). Conclusions The abnormal circadian rhythm in elderly hypertensive patients is closely related to early renal damage.

Objective To discuss the effect and possibility of NIPPV in ILD combined with respiratory failure. Methods A total of 37 cases of ILD combined with respiratory failure admitted by our hospital during Feb 2004 to Oct 2007 were divided into treatment group (20 cases) and control group (17 cases). Routine pharmaceutical intervention was adopted in both groups while NIPPV was given in the patients of treatment group. The symptoms、signs and the arterial blood gases were observed and analyzed. Results Among 20 patients,3 gave up treatment , 17 patients were successfully treated with the NIPPV therapy. There were significant improvement of PaCO2、PaO2、pH and respiratory rate ,heart rate after 2h NIPPV. Conclusion NIPPV is an effective method for ILD combined with respiratory failure,which can save the patients life and decrease the complications.

Objective To investigate the effects of interferon-α (IFN-α) and gefitinib on the proliferation and apoptosis of human colon cancer cell line HCT116. Methods Colon cancer cell line HCT116 was selected as research objective. The biological effects of IFN-α and gefitinib alone or combined on the cells were observed at different time point (after worked for 24, 48 and 72 hours). The proliferation inhibition of the medicine on the HCT116 cells was measured by methyl thiazolyl tetrazolium (MTT) assay. Morphologic changes were observed under optical microscope. Apoptosis was measured by flow cytometry (FCM). The results were analyzed with SPSS 13.0 software, two groups compare was tested by t test, and single factor variance analysis was for multiple group data compare. Results IFN-α and gefitinib alone or combined could significantly inhibit the proliferation of HCT116 cells (P<0.05), and there was a time and dose-dependent manner between the degree of inhibition and the working time and concentration of the medicine. With the work of the medicine, apoptosis morphologic changes were observed in the cells. And FCM result indicated that the apoptosis rate significantly increased. After treated with IFN-α and gefitinib alone or combined for 72h, the cell apoptosis rate were 15.6%±0.6%, 13.6%±0.4% and 31.2%±0.3% respectively, which was obviously higher than control group (6.8%±0.3%, P<0.05). Conclusion Both IFN-α and gefitinib were able to inhibit the proliferation and induce apoptosis of HCT116 cells moreover, and a synergistic effect was observed while combine used there two medicines.

Purpose To investigate the correlation of Dock180 and miR-31 expression in breast cancer cells,and to observe the effect of miR-31 on the invasion of breast cancer cells by Dock180.Methods MiR-31 was transfected into breast cancer cells by liposome transfection technique.The actual binding site of miR-31 to the 3'-untranslated region of Dock180 was confirmed through luciferase assay.Western blot was performed to detect the expression of Dockl80 and other related proteins.Real-time PCR was used to measure the expression of Dock180.Matrigel invasion were performed to detect the invasion of breast cancer cell lines with miR-31 increased.Resuits The protein levels of Dock180 in breast cancer cell lines negatively correlated with miR-31 expression,and Dock180 was directly targeted by miR-31.Dock180 downregulation and miR-31 overexpression reduced breast cancer cells invasion.Conclusion Dock180 modulated by miR-31 plays an important function in breast cancer cell lines invasion.

0.05) after irradiation with low dose of UVB. When the dose of UVB reached 60 mJ/cm2, the mRNA and protein expression of both p21 and Bax increased significantly (both P

Aim To assay the possible targets of adriamycin (ADM), screening ADM resistance related proteins.Methods The drug sensitivity of the cells was analyzed by IC50 assay;RT-PCR assay was used to detect the expression of genes in the cells;CMPK1 protein expression was tested by Western blot assay;the expression of CMPK1 in the cells was decreased by siRNA of CMPK1.Results Data from IC50 assay showed the sensitivity of cells transfected with CMPK1 was increased most(IC50 HEK293-CMPK /IC50 HEK293-Control=0.15, P<0.01), and the expression of CMPK1 protein in ADM resistant breast cells (MCF7/ADM) was lower than that in parent MCF7 cells (P<0.05).When the expression level of CMPK1 was decreased by CMPK1 siRNA, the sensitivity of MCF7 cells to ADM decreased (IC50 MCF7-siCMPK1/IC50MCF7-Control=3.6, P< 0.01), and the sensitivity of MCF7 cells to paclitaxel and gemcitabine also decreased.Conclusions CMPK1 was related to the multidrug resistance of cells, and the expression of CMPK1 was positively related to the sensitivity to drugs, which provides the possibility of CMPK1 as a target in the treatment of multidrug resistance.

Objective To investigate pulmonary function in terms of tidal respiration and the influencing factors on it in ＜ 34 weeks premature infants with RDS at corrected gestational age of 40 weeks.Methods A total of 49 of ＜ 34 weeks premature infants with RDS (RDS group) and 36 of ＜ 34 weeks premature infants without RDS (non-RDS group) followed throughout entire twelve months were collected fromn December 2013 to October 2015.Of them,35 RDS patients and 20 non-RDS patients had the pulmonary function examination.A total of 26 full term infants with hyperbilirubinemia (full term group) were recruited for comparison study.The differences in parameters of tidal respiration were compared among the three groups.The RDS patients were further divided into the mild RDS subgroup and severe RDS subgroup according to the severity of illness.Result ①The TPEF [(0.17 ± 0.04) s vs.(0.23 ± O.09) s],VPEF [(6.74±2.70) mLvs.(9.33±2.92) mL],TPEF/TE [(29.06±4.21)％ vs.(38.27± 7.16) ％],VPEF/VE [(32.54 ± 4.43) ％ vs.(39.64 ± 5.88) ％] in RDS group were significantly lower than those in full term group (P ＜0.05).The TPEF [(0.19 ±0.06) s vs.(0.23 ±0.09) s],TPEF/TE [(30.31 ±11.53)％ vs.(38.27±7.16)％],VPEF/VE [(34.39±8.44)％ vs.(39.64±5.88)％] in non-RDS group were significantly lower than those in full termn group (P ＜ 0.05).The TPEF,TPEF/TE,VPEF/VE in RDS group were lower than those in non-RDS group,but the differences were not significant (P ＞ 0.05).②The TPEF,VPEF,TPEF/TE,VPEF/VE in mild RDS group were higher than those in severe RDS group,but the differences were.not significant (P ＞ 0.05).③ Logistic regression analysis indicated that the gestational age was the protective factor of pulmonary function in premature infants with RDS.Conclusions Small airway resistance in ＜ 34 weeks premature infants with RDS is higher than that in full term infants.There was no significant difference in pulmonary function between RDS premature infants and non-RDS premature infants.The gestational age was the influencing factor of pulmonary function in premature infants with RDS.