Objective:To detect and analyze the mycoplasma infection in human laryngeal carcinoma and to explore the relationship between the infection with the development,progression and prognosis of laryngeal cancer.Methods: Immunohistochemical methods were employed to detect the mycoplasma infection in 214 specimens of different laryngeal lesions and cervical lymphatic tumors metastasized from laryngeal cancer,including 121 laryngeal cancer specimens,21 laryngeal precancerous specimens,17 vocal cord polypus specimens,14 normal laryngeal tissues adjacent to cancer tissues,9 normal laryngeal tissues opposite to the cancer,and 32 cervical lymphatic tumor specimens metastasized from laryngeal cancer.The infection results were subjected to statistical analysis.Results: The positive rates of mycoplasma 2G10 antibody in laryngeal carcinoma specimens,cervical lymphatic tumor specimens metastasized from laryngeal cancer,laryngeal precancerous specimens,vocal cord polypus specimens,normal laryngeal tissues adjacent to cancer tissues,and normal laryngeal tissues opposite to the cancer were 44.63%(54/121),34.38%(11/32),23.81%(5/21),17.65%(3/17),7.14%(1/14),and 0%(0/9),respectively,with those of laryngeal carcinoma specimens and cervical lymphatic tumor specimens metastasized from laryngeal cancer significantly higher than those of other specimens(P

Acupuncture Points ; Acupuncture Therapy ; Essential Tremor ; therapy ; Humans ; Male ; Young Adult

In older to study the interrelationship between laryngeal carcinoma and human papillomavirus (HPV). The distribution and expression of genome types of HPV in laryngeal carcinoma were investigated. HPV DNA of 160 cases of fresh tissue samples with different lesions of the larynx was detected by applying consensus primers and multiple primers of polymerase chain reaction (PCR) . The consensus primers used were able to detect 9 types of HPVs DNAs such as HPV6, 11, 16, 18, 31, 33, 34, 42 and 58. The results turned out to be as follows: The rate of positive cases with HPV infection was 49.3% (35/71) for the group of laryneal carcinoma, 22.7% (5/22) for the group of neck metastatic lymphnode, 11.8% (2/17) for precarcinomous lesions and 6.7% (2/30 ) for the group of vocal cord polypus, 20 case of normal laryngeal tissue adjacent to the carcinoma was HPV DNA negative. The results demonstrated that the occurrence and development of laryngeal carcinoma were closely related to HPV infection.

Objective To optimize the conditions of extraction process of polysaccharides from seeds of Toona sinensis; To preliminary determinate the antioxidant activity in vitro. Methods The content of polysaccharides from Toona sinensis was chosen as evaluation index; the extraction time, material liquid ratio, and extraction times were chosen as factors; L9(34) orthogonal design was used to optimize extraction process; antioxidant activity of polysaccharides from Toona sinensis was investigated by the total reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) method. Results From the statistical data of processing, the optimal extracting conditions were as follows:liquid to material was 1:12, extraction time was 120 min, and extracted for 3 times. Polysaccharides from seeds of Toona sinensis had certain reducing capacity and had better scavenging ability on DPPH? and the scavenging rate significantly increased with the concentration. The effect was simulated by curve equation. Conclusion The process is simple, feasible, stable and reliable and can be used for the small-scale study on extraction of polysaccharides from seeds of Toona sinensis. Polysaccharides from seeds of Toona sinensis have antioxidant activity.

ObjectiveTo investigate the pharmacokinetic effects of baicalin on cerebral ischemia-reperfusion (I/R) after iv administration in rats.MethodsThe cerebral I/R rats were induced by occluding the bilateral carotid arteries of normal rats for 2 h,followed by reperfusion.The resultant animals were immediately iv administrated with baicalin (90 mg/kg),whilst the same dose of baicalin was injected to the normal rats.Plasma samples were collected at different time to construct pharmacokinetic profiles by plotting drug concentration vs time.Quantification of baicalin in rat plasma was achieved using a simple and rapid HPLC method.ResultsIn normal rats,the major parameters of distribution half-life,elimination half-life,area under the plasma concentration-time (AUC),apparent volume of distribution (Vd),and clearance (CL),estimated by an open two-compartmental model,were 0.8868 min,26.0968 min,149.6204 mg/min·L,4.765 L/kg,and 0.5776 L/kg·min),respectively.However,in I/R rats,the corresponding parameters were 2.084 min,34.4998 min,260.0188 μg.min/L,5.9376 L/kg,and 0.334 L/(kg·min),respectively.ConclusionThe cerebral I/R could significantly increase AUC and Vd values,decrease CL values,and prolong the terminal half-life of baicalin.These findings suggest that the injuries of I/R could play an important role in pharmacokinetic process ofbaicalin.

Objective To study the clinical significance of MR imagings features in the diagnosis on central neurocytoma ( CNC ) . Methods From January 2010 to December 2012,9 patients with CNC were analyzed and examined by MRI before surgery,then received postoperative pathology examination. Results CNC were in the left lateral ventricle of 9 patients, closely linked with the Monro hole, of which 4 cases were in the first 2/3 of central ventricle of the left lateral ventricle,3 cases in the septum pellucidum and growth to bilateral ventricles,2 cases of infiltrating in the septum pellucidum and base side adhesion. For CNC,MRI signal was not uniform,solid part T1WI showed equal or slightly low signal,multiple cysts and signal cord like structure with the ventricular wall and septum pellucidum adhesion. By contrast-enhanced CT scan,there were the solid part heterogeneous obvious enhancement in 4 cases,moderate and slight uneven enhancement in 3 cases and 2 cases respectively. Uniform size,round or oval cells were showed by HE staining,and the synaptophysin was positive in 6 ca-ses by immunohistochemical staining,positive expression of glial fibrillary acidic protein in 3 cases. After operation,3 patients were lost to fol-low up,for 6 cases were followed up,survival of 2 cases in 3 years,4 cases in 2 years. Conclusion The results suggest that MRI display is located near the lateral ventricle central Monro hole before and lesions suggestive of CNC in young patients. For most CNC,synaptophysin has positive expression by immunohistochemical staining.

In order to definitude the influence caused by the different sampling in voice assessment.Method:We comparing the results acquired by total section and subsection sampling.Result:The results acquired by subsection tended to normal more than those acquired by total section. Conclusion:Subsection sampling voice assessment might conceal the drgree of the disease state of patients

OBJECTIVE Study the role of mycoplasma infection and expression of Ki67 protein in the pathogenesis, development and prognosis of laryngeal carcinoma. METHODS Immunohistochemistry method was used to study 145 specimens of laryngeal carcinoma tissues, 25 specimens of precarcinoma tissues, 31 specimens of vocal cord polyps and 15 specimens of normal tissues adjacent to laryngeal carcinoma. RESULTS ①The positive rates of PD4 and Ki67 were 45.52%(66/145) and 82.76 % (120/145) in laryngeal carcinoma tissue, 16.00 % (4/25) and 32.00 % (8/25) in precarcinoma tissue, 12.90 % (4/31) and 22.58 % (7/31) in vocal cords polyps, 6.67 % (1/15) and 0 (0/15) in normal tissues adjacent to laryngeal carcinoma. ②The positive rates of PD4 and Ki67 were higher in the advanced laryngeal carcinoma cases than that in the early laryngeal carcinoma cases. The positive rates of PD4 and Ki67 were higher in laryngeal carcinoma cases with cervical metastasis than that laryngeal carcinoma cases without cervical metastasis(P

OBJECTIVE: To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa. METHOD: To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs. RESULT: Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro. CONCLUSION OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.
Animals ; Cell Culture Techniques ; Cell Movement ; Cells, Cultured ; Humans ; Olfactory Mucosa ; cytology ; Rats

OBJECTIVE To construct a prokaryotic expression plasmid encoding HPV16E7-HSP70 fusion gene for further study on the immunity of HPV16E7- HSP70 fusion protein against laryngeal carcinoma. METHODS HPV16E7 was PCR-amplified,digested by NheI and SacI,and ligated into pET28a. HSP70 was cloned into pGEMTeasy,then recut from the vector by SalI and NotI and ligated into pET28a-HPV16E7. PCR amplification, restrict enzyme digestion, DNA sequencing, IPTG induction and Western Blot were used to identify the recombinant plasmid. RESULTS Double digestion and PCR amplification of the recombinant plas- mid have shown that the size of the inserted fragment is as expected. Sequence analysis has demonstrated that the inserted fragment encodes for the HPV16E7- HSP70 fusion gene. IPTG induction and Western Blot have shown that the fusion protein is expressed suc- cessfully in the prokaryotic expression plasmid. CONCLUSION The recombinant prokaryotic expression plasmid pET28a-HPV16E7-HSP70 has been con- structed successfully.